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Citations for BioLegend :
2501 - 2550 of 3486 citations for IL 12B Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated with plate-bound CD3 and soluble CD28 antibodies with the presence of 20 ng/μl IL-2 (Biolegend #589106) for 3 days before infection with HIV-1 reporter viruses ...
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bioRxiv - Immunology 2019Quote: ... cells were permeabilized and stained with IL-10 (clone JES5-16E3, eBioscience) or isotype control (Rat IgG2b Κ Isotype Control, Biolegend). Fluorescence intensities were measured on a FACSCalibur and at least 25,000 events for lung tissue were analyzed using FlowJo.
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Immunology 2021Quote: ... permeabilized with saponin 0.2% for 20 min and stained with PE-Cy7-anti-IFN-γ mAb and/or PE-anti-IL-10 mAbs (Biolegend). For TF and GzB/PRF staining ...
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bioRxiv - Cancer Biology 2020Quote: ... were treated with 10 Gy (as described above) and either PMX205 or vehicle and either IL-10 blocking antibody (LEAF Purified clone JES5-16E3, Biolegend #505012) (50 μg 24 hours before irradiation ...
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA ...
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bioRxiv - Immunology 2024Quote: Production of IFNγ and IL-10 from T cell culture supernatant was measured using Legend Max ELISA Kit Mouse IFNγ (BioLegend, 430807) and Mouse IL-10 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... PE-Cyanine7-conjugated anti-mouse Ly6G (E-AB-F1108H, elabscience, China) or intracellular staining for 488-conjugated anti-mouse IL-17A (506910, BioLegend, USA) was performed according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... CD19+ and CD4− CD8− CD19− cells were resuspended in R-10 media (RPMI + 2 mM L-glutamine + 10% FBS + Penicillin/streptomycin) + 20 U/ml IL-2 (Biolegend, https://www.biolegend.com). Primary monocytes were resuspended in R-10 media ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... prior to staining with the mAbs against cytokines (PE anti-IFN-γ, AF488 anti-TNF-α and PE-Cy7 anti-IL-2) (Biolegend) with Perm/Wash buffer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated anti-mouse TNF-α (clone MP6-XT22) and Pe-Cy7–conjugated anti-mouse IL-2 (clone JES6-5H4) antibodies (BioLegend, UK) for 20 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng/ml Escherichia coli lipopolysaccharides (LPS) for 24 hrs to induce M1 polarization or 25 ng/ml IL-4 (BioLegend catalog #766202) and 25 ng/ml IL-13 (BioLegend catalog #571102 ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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bioRxiv - Immunology 2023Quote: Cytokine production was quantified from cell supernatants or serum using the following ELISA kits: ELISA MAX Deluxe Set Mouse IL-10 (BioLegend, Cat# 431414), ELISA MAX Deluxe Set Human IL-10 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated for the period of activation expansion with 20µg/ml of anti IL-10 antibody (JES5-2A5; Biolegend catalog No. 504908) or corresponding isotype control (Biolegend catalog No ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Immunology 2024Quote: ... in a 5% CO2 incubator at 37 °C for 7 days with 30 ng/ml IL-15 (BioLegend, San Diego, CA, USA) and 10 ng/ml IL-12 (Peprotech ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained with biotin anti-human CD326 (Cat#324216; BioLegend; 1:250) for 30 minutes at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... 27] with specific markers including PE-conjugated mouse anti-human CD27 (Biolegend, CA, USA) and APC-conjugated mouse anti-human IgD (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... and after washing incubated with surface markers PerCP/Cy5.5 anti-human CD20 (BioLegend, 302326), PE-CF594 Mouse Anti-Human CD27 (BD BioScience ...
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bioRxiv - Developmental Biology 2021Quote: ... using PE-conjugated anti-human TRA-1-60-R antibody (Biolegend, catalog no. 330610).
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bioRxiv - Immunology 2020Quote: ... Brilliant Violet 421™ mouse anti-human CD69 (1:40 dilution, Clone FN50, BioLegend), PE mouse anti-human CD71 (1:20 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... MM samples were co-stained with mouse anti-human CD138-Alexa647 (Biolegend, clone MI15). PLA was scored manually in CD138+ cells in a blinded fashion as either – or + ...
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bioRxiv - Bioengineering 2022Quote: ... PE-conjugated mouse anti-human CD279 (PD-1) antibody (BioLegend, Santiago, CA, United States), FITC mouse anti-human TCRαβ (BD Biosciences ...
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bioRxiv - Genomics 2020Quote: ... 564219) for 5 minutes and stained with directly conjugated anti-human CD45 (Biolegend 304006) and EPCAM (eBioscience 25-9326-42 ...
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bioRxiv - Immunology 2021Quote: ... BV605 anti-human HLA-DR (clone L243; dilution 1:100; Biolegend; Cat no 307640); BV785 anti-human CD38 (clone HIT2 ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD8a (clone RPA-T8; dilution 1:100; Biolegend; Cat no 301050); Alexa Fluor 647 anti-human CD137 (clone 4B4-1 ...
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bioRxiv - Genomics 2019Quote: ... using antibodies specific to human blood cell surface antigens for CD45 (Biolegend, catalog# 103115), CD3 (Biolegend ...
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bioRxiv - Immunology 2019Quote: ... and stained with an AF647-labeled anti-human IgG Fc antibody (Biolegend, catalog 409320). Cells were then analyzed by flow cytometry on LSRFortessa analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2019Quote: ... anti-human PD-1 conjugated to APC/Cy7 (clone EH12.2H7, cat. no. 329921, BioLegend) and anti-human HLA-DR conjugated to eVolve 605 (clone LN3 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated with human or murine cytokines or chemokines (BioLegend, San Diego, CA) for 24 h prior to cell extract preparation ...
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bioRxiv - Biochemistry 2020Quote: ... Antibodies used were FITC-rat anti-mouse/human CD45R/B220 (clone RA3-6B2, BioLegend), and rat anti-mouse CD4 F(ab’)2 fragment (clone GK1.5 ...
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bioRxiv - Bioengineering 2021Quote: ... or PD-L1, purified anti-human CD274 (B7-H1, PD-L1) antibody (329701, Biolegend), were added at a 1:100 dilution for 24-48h and stored at 4°C ...
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bioRxiv - Bioengineering 2021Quote: The samples were thawed and analyzed using the LegendPlex Human CD8/NK Panel (Biolegend), according with the manufacturer instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then stained by an APC anti-human CD69 antibody (BioLegend, 310910) and analyzed by flow cytometry ...
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bioRxiv - Biophysics 2021Quote: ... PE/Dazzle ™ 594 anti-human PD-1 (CD279, Clone EH12.2H7, Biolegend, CA, USA), mouse anti-human PD-L1 (primary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... and the cells were washed and incubated with anti-human CD66b FITC (Biolegend #305104) and anti-human Semaphorin 7A-AF647 (R&D #FAB20681R ...
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bioRxiv - Microbiology 2020Quote: ... Analytes were quantified using the LEGENDplex Human Inflammation Panel 1 (Biolegend, San Diego, USA) according to the supplier’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and APC-labeled mouse anti-human PD-L1 (clone 29E2A3, Biolegend, San Diego, CA). The following anti-mouse antibodies we used for surface staining ...