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Citations for BioLegend :
2501 - 2550 of 8621 citations for 6 METHYL 7 1 METHYL 2 OXO PROPOXY 2 3 DIHYDRO 1H CYCLOPENTA C CHROMEN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... We lysed RBCs by adding 4 mL of 1X RBC lysis buffer (Biolegend, catalog #420301) to the resuspended pellet ...
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bioRxiv - Immunology 2021Quote: ... anti-IL-4 Brilliant Violet 421 and anti-IFN-γ PE (Biolegend. San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... CD127-BV785 (A019D5) and CD137-PE (4-1BB) (purchased from BioLegend, San Diego, CA, USA), CXCR5 (CD185)-PE-eFluor 610 (MU5UBEE ...
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bioRxiv - Neuroscience 2024Quote: ... and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend) and 50 ng/ml rhGM-CSF (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ and IL-4 production were determined using ELISA kits (BioLegend, San Diego, CA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... USA) cells were incubated with anti-PD-L1 blocking antibody (Ab; 4 μg/mL; BioLegend #29702 ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 20 min in 150 μl 4% paraformaldehyde (BioLegend; cat. no., 420801) at 4 °C in darkness before a 30-min permeabilisation step (eBioscience ...
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.5 µg of a unique TotalSeq-C CD298/β2M hashtag antibody (clone LNH-94/2M2, BioLegend) for each sample (n=5 ...
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bioRxiv - Neuroscience 2022Quote: ... binding the epitope 103-108 still present in C-terminal truncated forms of α-synuclein (Biolegend, Inc.). In brief ...
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bioRxiv - Immunology 2023Quote: ... and incubated at 37°C for two hours in the presence of murine CXCL12 (200ng/ml, Biolegend) or CXCL13 (1μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Endoderm yield was determined by dissociating EBs and evaluating CXCR4 and c-KIT (Biolegend, San Diego, CA) co-expression by flow cytometry on day 4 ...
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bioRxiv - Genomics 2023Quote: ... centrifuged (350 X g for 7min at 20°C) and washed once with cell staining Buffer (BioLegend). The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... 1-16 (1:1000, Biolegend). Sections were viewed ...
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bioRxiv - Cell Biology 2020Quote: ... Sca-1 (1:200; BioLegend), and Ter119 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... PD-1 (BioLegend, 1:100), phopho-STING (ThermoFisher 1:100) ...
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bioRxiv - Microbiology 2020Quote: ... The blot was then washed 3 times in PBS-T and probed with goat anti-rat HRP (Biolegend) and goat anti-mouse HRP (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 3 times in FACS buffer and incubated in Cyto-Fast Fix/Perm Buffer (BioLegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2021Quote: ... The first surface marker panel included: BV421 and PerCP/Cy5.5 anti-TIM-3 (clones B8.2C12 and RMT3-23, BioLegend), BV510 anti-PD-1 (EH12.1) ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3× before being incubated with APC-conjugated rat anti-CD83 antibody (1.25 μL/10×106 cells; Biolegend, 121510) for 30 minutes on ice ...
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bioRxiv - Synthetic Biology 2024Quote: ... and stained for the intracellular accumulation of IFNγ and TNFα (Supp. Table 3) in 1x Permeabilization Buffer (Biolegend). Samples were analyzed using a Fortessa LSR flow cytometer (BD ...
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bioRxiv - Immunology 2024Quote: ... and blocked using 3% FCS or mouse serum in Facs buffer and/or Tru block monocyte blocker (Biolegend) as required ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were again washed 3 times before adding 50 µL per well of HRP Streptavidin (#405210, BioLegend, USA) diluted 1:2000 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... incubated with secondary antibody diluted in 3% BSA PBS (Alexa Fluor 488 Goat anti-mouse IgG, BioLegend, 405319) in the dark for 1 hour at room temperature and washed 3x with PBST ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation (“in vitro-derived”) were washed and labeled using CFSE (BioLegend). Murine bone marrow neutrophils were isolated by negative selection (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... After 4 hours BMDMs were harvested and stained with an antibody against F4/80 (BM8, Biolegend) and propidium iodide (BD Biosciences) ...
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bioRxiv - Immunology 2019Quote: ... macrophage activation was induced by adding either IL-4 (20 ng/ml, Biolegend, San Diego, USA), IL-13 (20 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend) were added to the neutralized FA fraction ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was removed by aspiration and 500 microliters of fixation buffer (4% paraformaldehyde in PBS, Biolegend) added to each well and the slide kept in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... at 10 ng/mL (growth media) and 4 µg/mL of anti-CD28 antibody (Biolegend, #302934). Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-4 antibody (clone 11B11, Biolegend, #504122), 10 ng/ml animal-free recombinant murine IFNγ (PeproTech ...
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bioRxiv - Microbiology 2021Quote: ... malayi were individually incubated at 37 °C in RPMI 1640 with L-glutamine with 125 nM DRAQ5 (Biolegend) for 24-48 hrs ...
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bioRxiv - Cancer Biology 2021Quote: ... c-Met and CK antibodies were exchanged with the following clones to detect mouse epitopes: EpCAM: G8.8 (Biolegend), c-Met ...
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bioRxiv - Microbiology 2020Quote: ... adherent monolayers were detached by incubation at 37°C for 5 mins with Accutase Cell Detachment Solution (BioLegend). After washing ...