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Citations for BioLegend :
2451 - 2500 of 10000+ citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cell suspensions were then stained using the directly-labelled primary antibody TNFα_PE (BioLegend; 502929) or mouse IgG1 (eBiosciences ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were then incubated in staining buffer with APC anti-mouse Cd2 Antibody (BioLegend 100112 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with human KI67-APC antibody (Biolegend, Cat#151206, clone 11F6).
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bioRxiv - Cancer Biology 2023Quote: ... Human leukemia cells were labelled with a FITC anti-human CD45 antibody (368508; BioLegend) then sorted using a BD FACS Aria II Cell Sorter.
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell suspensions were stained with Phycoerythrin-conjugated anti-CD19 antibody (clone 6D5, BioLegend, San Diego CA in preparation for flow cytometry ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated overnight with primary antibodies Nestin (Biolegend, Cat# 839801, 1:200), MAP-2 (Santa Cruz ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2024Quote: ... fixed and permeabilized cells were incubated with human-specific APP antibody (SIG-39320, Biolegend) at dilution 1:250 for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... cells were suspended in BV711-CD11B antibody (macrophage marker; BioLegend, 301344, 1:20 dilution) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... library preparation and sequencing: Cell suspensions were labelled with TotalSeq-B hashtag antibodies (BioLegend) following a modified version of the manufacturer’s sample multiplexing protocol (https://tinyurl.com/ycy5wah8) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pre-incubated with anti-mouse CD16/CD32 antibody (Cat. # 101302 from Biolegend) for 5 minutes to block Fc receptors ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 μL antibody per million cells in 100 μL staining buffer (BioLegend, #420201) were used in the staining according to the standard protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with APC anti-human HLA-A,B,C antibody (BioLegend, #311410) before FACS ...
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bioRxiv - Cancer Biology 2024Quote: ... the cell pellets were stained with anti-GzmB antibody (FITC, clone QA16A02, BioLegend, #372206), followed by flow cytometry analysis.
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bioRxiv - Immunology 2024Quote: ... TotalSeq-C0255) (0.5 µg per 1,000,000 cells) and TotalSeq-C0050 anti-CD19 antibody (BioLegend) for demultiplexing individual samples in downstream analysis ...
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bioRxiv - Immunology 2024Quote: ... cells were incubated with biotinylated antibodies (anti-mouse CD3e, CD19, CD11b; 1:300; Biolegend) in 100 μL of wash buffer for 20 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and then stained with the fluorochrome-labeled anti-mouse CD3/CD4/CD8/CD44/CD62L mAbs (all purchased from Biolegend except CD44 from BD). Cells were then treated with Cytofix/Cytoperm (BD ...
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bioRxiv - Genetics 2021Quote: ... we negatively isolated memory T cells with a modified Pan T cell magnetic-activated cell sorting (MACSR) kit with anti-CD45RA biotin and followed an optimized version of the CITE-seq protocol with TotalSeqTM-A (BioLegend) oligonucleotide-labeled antibodies for a panel of 31 surface proteins (43) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Immunology 2020Quote: ... or biotin, 2G5, Beckman Coulter, or Ber-ACT8, Brilliant Violet 711, BUV395, BD Biosciences, or Ber-ACT8, PE-Cy-7, Biolegend), KIR2DL1 (FAB1844F ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Immunology 2023Quote: ... samples from animals that were vaccinated with the recombinant GPCysR4-I53-50A.30 To exclude I53-50A-binding B cells we included a SARS-CoV-1 Spike probe containing I53-50A-biotin which was labeled with streptavidin AF647 and streptavidin BV421 (both Biolegend). After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD8 (biotin, clone: 53-6.7), CD205 (clone: NLDC-145) and TNF-α(PE, clone: MP6-XT22) were purchased from BioLegend. The CD18-reactive mAb M18/2 was purchased from Biozym ...
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bioRxiv - Immunology 2023Quote: ... Panels 2 and 3 incorporated anti-Digoxygenin (RD System, #611621), anti-FITC (Standard BioTools, #3174006B) and anti-Biotin (Biolegend, #409002) metal-tagged antibodies to detect the RNAscope staining output.
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were selected using hygromycin and the stable population was sorted on our single cell platform for CD19 expression levels by staining transfected cells with AlexaFluor® 647 anti-CD19 antibody (#302222, Biolegend). Cells were sorted for different expression levels of CD19 and clonal cell lines with expression levels comparable to that of standard CD19 positive cell lines such as Raji ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Cancer Biology 2020Quote: ... followed by sorting of GFP- cells and magnetic negative selection-were incubated with Cell Hashing HTO-conjugated antibodies (Biolegend, TotalSeq(tm) anti-mouse Hashtag reagents Cat# A0301-A0306 ...
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bioRxiv - Immunology 2021Quote: ... cells were initially blocked with anti-mouse Fcγ III/II receptor (CD16/CD32) antibodies and dead cells excluded by staining with a fixable live/dead cell-exclusion dye (BioLegend, USA). Then ...
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bioRxiv - Bioengineering 2020Quote: PDCD1 and B2M expression on EL4 cells were assessed with fluorescent antibody staining using an APC-conjugated PD1 antibody (BioLegend, San Diego, CA: 135210) or AlexaFluor 647-conjugated H-2Kb antibody (BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... Peptides (400 μM) were mixed with Flex-T™ HLA-A*11:01 Monomer UVX (Biolegend), then subjected to UV light for 30 min on ice ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Immunology 2022Quote: ... Samples were washed in FACS buffer to remove unbound tetramer prior to surface staining with anti-TCR□β APC (clone IP26) and anti–CD7 FITC (clone CD7-6B7, Biolegend, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... were isolated from buffy coats by density gradient centrifugation (Lymphoprep, StemCell ref: 07851) and activated on plates coated with anti-CD3 (1 mg/ml, Biolegend, ref: 317347, clone: OKT3) and anti-CD28 (1 mg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... 25-4317-82) for HSC/MPP staining, or together with CD34-FITC (eBioscience, 11-0341-85) or CD34-biotin (BioLegend, 119304) and FcγR-PerCP-Cy5.5 (eBioscience ...
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bioRxiv - Molecular Biology 2020Quote: ... Linker oligo with 3’-terminal biotin was purchased from IDT (Coralville, Iowa) and purified streptavidin was purchased from BioLegend (Biolegend, #405150). Biotinylated antibody ...
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bioRxiv - Immunology 2019Quote: The percentage of CAR and ΔLNGFR positive T cells was assessed by biotin-conjugated rhCD19-Fc (Cat # CD9-H5259, ACRO Biosystems, USA) with AF647-conjugated streptavidin (Cat # 405237, Biolegend, USA), and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605 ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µL plasma was centrifuged at 10 000 g for 30 minutes and the total EV pellet washed once before resuspension with biotin-saturated MojoSort streptavidin magnetic nanobeads (Biolegend, USA) to deplete non-specific binding ...
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bioRxiv - Immunology 2021Quote: Cells were stained with a 198 Total-seq A-conjugated antibody panel for murine (BioLegend, see Table S4 for a list of antibodies ...
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bioRxiv - Immunology 2019Quote: ... and GC B cells were stained with antibodies against mouse B220 (Clone: RA3-6B2, Biolegend), GL7 (Clone ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with the following antibodies specific for murine: CD45 [clone 30.F11 (BioLegend)] ...
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bioRxiv - Genetics 2019Quote: ... and then stained with anti-FOXP3-BV421 antibody (5 µl/106 cells, clone 206D, BioLegend) using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... Cells were fixed and permeabilized for 30 minutes and stained with commercial antibodies from Biolegend, BD ...
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bioRxiv - Bioengineering 2020Quote: ... Tumor cells were analyzed for antigen expression with APC-conjugated anti-CS1 antibody (BioLegend #331809) or PE-conjugated anti-BCMA antibody (BioLegend #357503) ...
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bioRxiv - Immunology 2021Quote: ... B cells were also stained with following anti-human antibodies: anti-IgD-BV421 (Biolegend, 348226), anti-CD27-FITC (BD biosciences ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901] ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with an anti-FcγIII/II receptor antibody and Zombie NIR™ (Biolegend). Then surface staining was performed on the cells with antibodies against CD45 (30-F11) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated with fluorophore-conjugated antibodies CD11b BV605 (1:200, clone M1/70, Biolegend) and CD11c APC (1:100 ...