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2451 - 2500 of 3651 citations for Annexin V Solution APC since 2020

• In vivo CRISPR screens identify key modifiers of CAR T cell function in myeloma

  Felix Korell, et al., bioRxiv - Immunology 2024

  Quote: ... NGFR (Mouse anti-Human, Biolegend, APC Clone ME20.4, Cat-no. 345108), CD34 (Mouse anti-Human ...
• Basement membrane hydrogels dampen CAR-T cell activation: nanofibrillar cellulose gels as alternative to preserve T cell function in 3D cell cultures

  Sonia Aristin Revilla, et al., bioRxiv - Immunology 2024

  Quote: ... Murine CD4+ T cells were stained with anti-CD4-APC (BioLegend) and anti-CD25-Pacific blue (BioLegend) ...
• Plasmodium falciparum expresses fewer var genes at lower levels during asymptomatic dry season infections than clinical malaria cases

  Sukai Ceesay, et al., bioRxiv - Microbiology 2024

  Quote: ... and 1:50 APC anti-human Lineage Cocktail (BioLegend, cat# 348703) in PBS for 30 min at 37 °C ...
• Hantaan virus-derived peptides that stabilize HLA-E could abrogate inhibition of CD56^dimNKG2A⁺ NK cells

  Manling Xue, et al., bioRxiv - Immunology 2024

  Quote: ... the APC-labeled anti-human CD107a mAb (Clone H4A3, BioLegend, USA) was added to the cells during stimulation ...
• Hantaan virus-derived peptides that stabilize HLA-E could abrogate inhibition of CD56^dimNKG2A⁺ NK cells

  Manling Xue, et al., bioRxiv - Immunology 2024

  Quote: ... APC-labeled anti-human HLA-DR mAb (Clone L243, BioLegend, USA) and FITC-labeled anti-human CD69 mAb (Clone FN50 ...
• Hantaan virus-derived peptides that stabilize HLA-E could abrogate inhibition of CD56^dimNKG2A⁺ NK cells

  Manling Xue, et al., bioRxiv - Immunology 2024

  Quote: ... APC/FireTM-labeled anti-human CD16 mAb (Clone 3G8, BioLegend, USA), PE-Cy7-labeled anti-human NKG2A mAb (Clone S19004C ...
• Pericytes mediate neurovascular remodeling in chronic arterial hypertension

  Lorena Morton, et al., bioRxiv - Neuroscience 2024

  Quote: ... and CD81 (APC-conjugated, Clones HI9A, H5C6 and 5A6, respectively, Biolegend). Samples were analyzed using an Attune NxT Flow Cytometer equipped with a small particle side scatter filter ...
• Human placental organoids as a model to probe early gestation maternal immune dynamics

  Emily M. Slaby, et al., bioRxiv - Bioengineering 2024

  Quote: ... anti-Granzyme B APC/FireTM 750 (BioLegend catalog #372210, clone QA16A02), anti-CD86 BV421 (BioLegend catalog #305426 ...
• Disruption of Immune Responses By Type I Diabetes Exacerbates SARS-CoV-2 Mediated Lung Injury

  Sara Kass-Gergi, et al., bioRxiv - Immunology 2024

  Quote: ... APC anti-mouse CD8a Recombinant antibody (1:100, Biolegend, Clone QA17A07), PE anti-mouse Ly-6G antibody (1:200 ...
• Intracortical injection of immune checkpoint inhibitor promotes monocyte/macrophage infiltration and restores microglial function and neuronal activity in an AD mouse model

  Taeyoung Park, et al., bioRxiv - Neuroscience 2024

  Quote: ... CD4-APC-Cy7 (1:200 dilution, cat# 100413, clone GK1.5, BioLegend), C-kit-PerCP (1:200 dilution ...
• Interleukin-1 receptor antagonist is a conserved driver of tuberculosis

  Dmitri I. Kotov, et al., bioRxiv - Immunology 2024

  Quote: ... The enriched samples were then stained with APC-labeled Streptavidin (BioLegend) for 15 minutes on ice before being stained with Sytox Green Dead Cell Stain (Thermo Fisher Scientific) ...
• Chlamydia muridarum Causes Persistent Subclinical Infection and Elicits Innate and Adaptive Immune Responses in C57BL/6J, BALB/cJ and J:ARC(S) Mice Following Exposure to Shedding Mice

  Noah Mishkin, et al., bioRxiv - Microbiology 2024

  Quote: ... APC/Fire 750 CD45R/B220 (RA3-6B2, catalog no. 103260, Biolegend), APC/Cyanine7 CD19 (6D5 ...
• Potent antitumor activity of a designed interleukin-21 mimic

  Jung-Ho Chun, et al., bioRxiv - Biochemistry 2024

  Quote: ... or APC anti-human IL-21R (1:100, Biolegend, Cat#347808) and BV421 anti-mouse/human granzyme B (1:100 ...
• Activity-based selection for enhanced base editor mutational scanning

  Eleanor G Kaplan, et al., bioRxiv - Genetics 2024

  Quote: ... cells were stained with APC anti-human CD274 antibody (BioLegend 393610). Staining was performed by seeding 150 µL of cells per well in a 96-well U-bottom plate ...
• PURE-seq identifies Egr1 as a Potential Master Regulator in Murine Aging by Sequencing Long-Term Hematopoietic Stem Cells

  Sixuan Pan, et al., bioRxiv - Bioengineering 2024

  Quote: ... and CD150 (SLAM) APC (clone TC15-12F12.2, BioLegend; 1:50 dilution). After adding the rat serum and isolation cocktail of the Lineage Cell Depletion Kit ...
• Peptide-enabled ribonucleoprotein delivery for CRISPR engineering (PERC) in primary human immune cells and hematopoietic stem cells

  Srishti U Sahu, et al., bioRxiv - Bioengineering 2024

  Quote: Anti-human beta-2-microglobulin-APC (BioLegend #316312, RRID:AB_10641281 Clone 2M2)
• Defining the Vascular Niche of Human Adipose Tissue Across Metabolic Conditions

  Ibrahim AlZaim, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 1:1000 CD45-APC-Vio770 (REA747, 130-110-635, Biolegend)) containing human IgG (Privigen ...
• Limitations of the radiotheranostic concept in neuroendocrine tumors due to lineage-dependent somatostatin receptor expression on hematopoietic stem and progenitor cells

  Nghia Nguyen, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The antibodies used were as follow: CD34-APC-Cy7 (Biolegend, 343514), CD38-FITC (Biolegend ...
• CXCL9, granzyme B and TNF-α orchestrate protective in vitro granulomatous responses across Mycobacterium tuberculosis complex lineages

  Ainhoa Arbués, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were incubated for 15 min at room temperature in 100 μl of Annexin binding buffer (ABB, BioLegend) containing APC-labeled Annexin V ...
• Differential repression of Otx2 underlies the capacity of NANOG and ESRRB to induce germline entry

  Matúš Vojtek, et al., bioRxiv - Developmental Biology 2021

  Quote: ... 1:200 v/v Alexa Fluor 647 anti-mouse/human CD15 (SSEA-1) (Biolegend, 125608) and 1:500 v/v PE anti-mouse/rat CD61 (Biolegend ...
• The Injury Response to DNA Damage Promotes Anti-Tumor Immunity

  Ganapathy Sriram, et al., bioRxiv - Cancer Biology 2020

  Quote: ... washed once in PBS containing 0.9 mM Ca2+ and 0.5 mM Mg2+ and then stained with AnnV-FITC for 15 minutes in Annexin binding buffer at room temperature according to the manufacturer’s protocol (Biolegend). Cells were co-stained with DAPI at a final concentration of 1 µg/ml for 2 minutes in Annexin binding buffer ...
• Broadly reactive human monoclonal antibodies targeting the pneumococcal histidine triad protein protect against fatal pneumococcal infection

  Jiachen Huang, et al., bioRxiv - Immunology 2021

  Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
• The generation of a Bcl11a lineage tracing mouse model

  Sara Pensa, Pentao Liu, Walid T. Khaled, bioRxiv - Developmental Biology 2021

  Quote: ... EpCAM-APC/Cy7 (Biolegend, clone G8.8, 0.5 µg ml−1, 1:500), Cd49f-BV421 (Biolegend 313623 ...
• A homeostatic interferon-lambda response to bacterial microbiota stimulates preemptive antiviral defense within discrete pockets of intestinal epithelium

  Jacob A. Van Winkle, et al., bioRxiv - Immunology 2022

  Quote: ... dissociated cells were enriched using MojoSort Mouse anti-APC Nanobeads (BioLegend, #480072) after flow cytometry staining for anti-EpCAM and anti-CD45 with APC fluorophores by following manufacturer protocols.
• Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cells

  M. Kyle Cromer, et al., bioRxiv - Molecular Biology 2020

  Quote: ... HSPCs were stained using CD34 APC (561; BioLegend, San Diego, CA, USA), Ghost Dye Red 780 (Tonbo Biosciences ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD56 APC Cy7 (1:50, clone HCD56, BioLegend, cat # 318332), Anti-human CD45 APC Cy7 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CXCR4 APC Cy7 (1:50, clone 12G5, BioLegend, cat # 306528), Anti-human CTLA4 APC Cy7 (1:25 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CTLA4 APC Cy7 (1:25, clone BNI3, BioLegend, cat # 369634), Anti-human CD56 APC Cy7 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD45 APC Cy7 (1:100, clone HI30, BioLegend, cat # 304014), Anti-human CD14 APC eF780 (1:100 ...
• Systematically optimized BCMA/CS1 bispecific CAR-T cells robustly control heterogeneous multiple myeloma

  Eugenia Zah, et al., bioRxiv - Bioengineering 2020

  Quote: ... cells were surface-stained with BCMA-PE and CS1-APC antibodies (BioLegend) to verify antigen knockout ...
• Macrophage-mediated antibody dependent effector function in aggressive B-cell lymphoma treatment is enhanced by Ibrutinib via inhibition of JAK2

  Verena Barbarino, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Macrophages were stained with F4-80 APC antibody (BioLegend, San Diego, USA) and cells were analyzed using MACSQuant flow cytometry.
• Metformin abrogates pathological TNF-α-producing B cells through mTOR-dependent metabolic reprogramming in polycystic ovary syndrome

  Na Xiao, et al., bioRxiv - Cell Biology 2021

  Quote: ... and subsequently stained with mouse TNF-α-APC (clone MP6-XT22; BioLegend) or human TNF-α-PerCP (clone Mab11 ...
• Open-source personal pipetting robots with live-cell incubation and microscopy compatibility

  Philip Dettinger, et al., bioRxiv - Bioengineering 2021

  Quote: ... CD41-PerCPeFL710 (eBioscience, Clone MWReg30) and CD105-APC (Biolegend, Clone MJ7/18). Cells were stained for 90 min on ice and sorted using a BD FACS Aria I or III with 70-μm nozzle ...
• An enhanced yeast display platform demonstrates the binding plasticity under various selection pressures

  Jiří Zahradník, et al., bioRxiv - Biochemistry 2020

  Quote: ... The streptavidin conjugated with APC was purchased commercially (Cat#405207, BioLegend, USA).
• Functional characterization of the biogenic amine transporter system on human macrophages

  Phillip M Mackie, et al., bioRxiv - Neuroscience 2021

  Quote: ... and stained for intracellular markers MCP1/CCL2 (Biolegend, 505909, APC, 1:100), TNF-alpha (Biolegend ...
• CAR-NK Cells Effectively Target the D614 and G614 SARS-CoV-2-infected Cells

  Minh Ma, et al., bioRxiv - Immunology 2021

  Quote: ... APC/Fire 750 anti-human CD226 antibody (DNAM-1) (clone 11A8, BioLegend), APC/Fire 750 anti-human KLRG1 (MAFA ...
• Small extracellular vesicles from young mice prevent frailty, improve healthspan and decrease epigenetic age in old mice

  Jorge Sanz-Ros, et al., bioRxiv - Physiology 2022

  Quote: ... sEVs were stained with 4µg/mL of APC anti-CD63 (Biolegend, 143905) for 30 minutes at 4°C in dark ...
• Targeting SARS-CoV-2 infection through CAR-T like bispecific T cell engagers incorporating ACE2

  Mikail Dogan, et al., bioRxiv - Bioengineering 2022

  Quote: ... then stained with APC anti-human IgG Fc Antibody clone HP6017 (Biolegend). Samples were acquired on a BD FACSymphony A5 analyzer and data were analyzed using FlowJo (BD Biosciences).
• A host–gut microbial co-metabolite of aromatic amino acids, p-cresol glucuronide, promotes blood–brain barrier integrity in vivo

  Andrew V. Stachulski, et al., bioRxiv - Systems Biology 2022

  Quote: Cells were labelled with APC-conjugated mouse monoclonal anti-CD11b (Biolegend, UK), FITC-conjugated mouse monoclonal anti-CD14 (Biolegend ...
• Phenotypic dissection of epithelial lineages and therapeutic manipulation of differentiation programs in human Adenoid Cystic Carcinomas (ACCs)

  Sara Viragova, et al., bioRxiv - Cancer Biology 2022

  Quote: ... anti-human/mouse CD49f-APC (BioLegend 313616, clone GoH3, dilution 1:40) and anti-human KIT-PE (BioLegend 313204 ...
• Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer progression

  Jason J. Northey, et al., bioRxiv - Cancer Biology 2022

  Quote: ... and TER-119-APC (BioLegend, clone TER-119, Cat: #: 116211, 1:80) and CD61-FITC (BioLegend ...
• Simulating the Tumor Microenvironment for Immune Cell Interactions via Deployable Extrusion Bioprinting

  Corrado Mazzaglia, et al., bioRxiv - Bioengineering 2022

  Quote: ... Primary antibodies for immune profiling: anti-B220 (Biolegend 103224, APC-cy7 conjugated), anti-CD4 (Invitrogen 25-0041-82 ...
• SARS-CoV-2-specific T cells exhibit phenotypic features reflecting robust helper function, lack of terminal differentiation, and high proliferative potential

  Jason Neidleman, et al., bioRxiv - Immunology 2020

  Quote: ... cells were stained with APC/Cyanine7 anti-human CD3 (Clone SK7, Biolegend), PE/Dazzle™ 594 anti-human CD4 (Clone RPA-T4 ...
• Loss of Rnf31 and Vps4b sensitizes pancreatic cancer to T cell-mediated killing

  Nina Frey, et al., bioRxiv - Cancer Biology 2021

  Quote: ... T-cells were stained with CD8a-APC (clone 53-6.7; Biolegend 100711) to separate both populations ...
• Single-domain antibodies for targeting, detection and in vivo imaging of human CD4⁺ cells

  Bjoern Traenkle, et al., bioRxiv - Immunology 2021

  Quote: ... IFN-γ AlexaFluor 700 (4S.B7) and CD154 APC (2431) antibodies (all BioLegend) (Widenmeyer et al. ...
• A functional screening platform for engineering chimeric antigen receptors with reduced on-target, off-tumour activation

  Raphaël B. Di Roberto, et al., bioRxiv - Immunology 2020

  Quote: ... and then with 1:250 APC-labeled anti-human HER2 antibody (Biolegend). The same antibody and concentrations were used to measure surface HER2 expression on cell lines SKBR3 ...
• A gut commensal protist protects against virus-mediated loss of oral tolerance

  Magdalena Siller, et al., bioRxiv - Immunology 2022

  Quote: ... I-A/I-E (M5/114.15.2, APC-Fire750, BioLegend, 11-5321-82), Foxp3 (FJK-16s ...
• Endo-lysosomal assembly variations among Human Leukocyte Antigen class I (HLA-I) allotypes

  Eli Olson, Theadora Ceccarelli, Malini Raghavan, bioRxiv - Immunology 2022

  Quote: ... and anti-CD209-APC (all used at 1:200 and from Biolegend), as well as anti-Bw6-FITC (Biolegend ...
• Endo-lysosomal assembly variations among Human Leukocyte Antigen class I (HLA-I) allotypes

  Eli Olson, Theadora Ceccarelli, Malini Raghavan, bioRxiv - Immunology 2022

  Quote: ... and anti-CD209-APC (all used at 1:200 and from Biolegend). After staining for 30 min on ice ...