5 Nitro 1H benzo de isoquinoline 1 3 2H dione citations for BioLegend

Quote: ... Sections were incubated with different primary antibodies (Rat-anti-MBP, Sigma-Aldrich MAB386, 1:1000, monoclonal; Rabbit-anti-GFAP, CiteAb Z0334, 1:1000, polyclonal; Mouse-anti-6E10 Amyloid-β, BioLegend SIG-39300, 1:1000, monoclonal) diluted in 200 μl 10% NDS and 0.4% Triton-X blocking medium ON at 4°C ...

Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer

Florian Uhlitz, et al., bioRxiv - Cancer Biology 2021

Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...

Protection from viral rebound after therapeutic vaccination with an adjuvanted DNA vaccine is associated with SIV-specific polyfunctional CD8 T cells in the blood and mesenteric lymph nodes

Hillary C. Tunggal, et al., bioRxiv - Microbiology 2020

Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...

HDAC1-3 inhibition reduces CCR2 expression and immunosuppressive function of myeloid-derived suppressor cells

Zhiqi Xie, et al., bioRxiv - Immunology 2023

Quote: ... T cell proliferation was stimulated with anti-CD3ε (1 μg/mL)/CD28 (1 μg/mL) antibodies (BioLegend). The anti-CD3ε antibody was coated on each well of the plate via overnight incubation at 4°C with PBS before coculture ...

Interpretable deep learning reveals the sequence rules of Hippo signaling

Khyati Dalal, et al., bioRxiv - Genomics 2024

Quote: ... at a 1:300 dilution with DAPI 2 μl in 1 ml of 10 μg/ml (BioLegend) and kept on light rotation for 2 h at RT ...

Spermidine suppresses DC activation via eIF5A hypusination and metabolic adaptation

Gavin R Meehan, et al., bioRxiv - Immunology 2023

Quote: ... CD40-APC (1:100) and CD11c-e450 (1:100) (all antibodies from Biolegend, San Diego, CA, USA). Cells were incubated with 100μl of the antibody suspension for 20 minutes at 4°C before a final wash in Ca2+/Mg2+ free PBS containing 2mM EDTA at x400g for 5 minutes at 4°C ...

Improved metabolic syndrome and timing of weight loss is crucial for influenza vaccine-induced immunity in obese mice

Rebekah Honce, et al., bioRxiv - Microbiology 2022

Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...

Defining the mechanisms of action and resistance to the anti-PD-1+LAG-3 and anti-PD-1+CTLA-4 combinations in melanoma flank and brain models

Manali S. Phadke, et al., bioRxiv - Immunology 2023

Quote: ... 1 × 106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...

Generation of a human Tropomyosin 1 knockout iPSC line

Madison B Wilken, et al., bioRxiv - Cell Biology 2023

Quote: ... Alexa-Fluor®-488 SSEA1 (CD15) (1:50) and Alexa-Fluor®-647 SSEA5 (1:200) (BioLegend). In all experiments ...

SR-BI regulates the synergistic mast cell response by modulating the plasma membrane-associated cholesterol pool

Sandro Capellmann, et al., bioRxiv - Immunology 2023

Quote: ... ST2 and CD13 was verified using FITC-coupled anti-FcεRI (1:100, clone MAR-1, BioLegend, #134306), PE-coupled anti-KIT (1:100 ...

Cytosolic S100A8/A9 promotes Ca²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

Matteo Napoli, et al., bioRxiv - Physiology 2024

Quote: ... and immunofluorescence staining for PECAM-1 (AlexaFluor488 labelled primary monoclonal rat antibody, 5μg mL-1, MEC13.3, BioLegend) and S100A9 (Cy5.5 directly labelled ...

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