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Citations for BioLegend :
201 - 250 of 5855 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Cell Biology 2019Quote: Pellet cells obtained from BAL (n= 5 mice/group in three different experiment) were incubated with a mix of conjugated antibodies (Biolegend) for the following T-cell subset superficial markers ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... T-25 or T-75 flasks were coated with 5 µg/mL anti-CD3ε antibodies (clone 145-2C11; 100340, BioLegend) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... for 5 minutes at room temperature prior to staining with a surface-stain cocktail containing the following antibodies purchased from BioLegend® (San Diego ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 min on ice and then stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat: 152406) CD95 (PE-Cy7 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with FACS buffer and pre-incubated with 5 μg/mL TruStain FcX anti-mouse CD16/32 antibody (clone 93, BioLegend) in 100 μL FACS buffer for 5 minutes on ice before immunostaining ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 and 5 μg/mL Brefeldin A (BFA; BioLegend) was added during the last 6 h of stimulation ...
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bioRxiv - Cancer Biology 2020Quote: ... washed once in PBS containing 0.9 mM Ca2+ and 0.5 mM Mg2+ and then stained with AnnV-FITC for 15 minutes in Annexin binding buffer at room temperature according to the manufacturer’s protocol (Biolegend). Cells were co-stained with DAPI at a final concentration of 1 µg/ml for 2 minutes in Annexin binding buffer ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with Annexin V-FITC (5uL in 0.14 mL Annexin V Binding Buffer, #640914; Biolegend, San Diego, CA) for 10 minutes at 4°C in darkness ...
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bioRxiv - Immunology 2023Quote: ... washed once in Annexin V buffer and resuspended in Annexin V binding buffer with Annexin V-BV421 (Biolegend; 640924) and incubated for 15 minutes at room temperature protected from the light ...
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bioRxiv - Cell Biology 2021Quote: ... IL-5 (BioLegend) and IL-13 (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Systems Biology 2019Quote: ... blocked in TBST buffer supplemented with 5% bovine serum albumin (BSA) and incubated overnight with primary anti-XBP1s antibody (Biolegend Poly6195) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... or double knockout cells were harvested and washed with PBS containing 1% bovine serum albumin (BSA) and incubated with 5 μL of PE-conjugated EphA2 antibody (Biolegend, SHM16) in 50 μL PBS containing 1% BSA for 30 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membrane was next added to 5% nonfat milk in TBST containing horseradish peroxidase-conjugate goat anti-mouse IgG1 secondary antibodies (Biolegend 405306) diluted at 1:5000 and incubated on a horizontal rocker for 60 minutes ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Immunology 2020Quote: ... were resuspended in 100μl protein blocking solution with 5 μl fluorescent conjugated antibodies, CD8 (Biolegend Cat# 100714, CD45 (Biolegend Cat# 103130), CD68 (Biolegend Cat# 137004) ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... spun at 700 g for 3 minutes at 4°C and washed in MACS Buffer 3× before being incubated with the following antibodies: APC-conjugated rat anti-CD3 (5 μL/10×106 cells; Biolegend, 100408), PE-conjugated rat anti-CD8b (2.5 μL/10×106 cells ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2024Quote: ... followed by 5 µL/well of the antibody cocktail for extracellular staining: anti-CD3-BV421 (clone 17A2) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Immunology 2022Quote: ... to block nonspecific binding and then were stained with dead dye to exclude dead cells (Aqua, ThermoFisher or Zombie NIR, Biolegend) for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... and the DAXL-88 (anti-AXL) binding assay used Alexa Fluor 647 AffiniPure anti-Fc tag Goat Anti-Human IgG (410711, BioLegend). Anti-Human CD4 antibody (BD ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: After 7 days of shRNA-mediated knockdown 1 x 105 cells were wash once in PBS and resuspend in Annexin-V Binding Buffer (422201, BioLegend) with 3 μl of Annexin-V-APC (640941 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728, 1:1000), F4/80 (Biolegend Cat # 123110 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2020Quote: ... Cells were surface stained and washed with FACS buffer then resuspended in 100 μL Annexin V binding buffer (Biolegend: 79998). Cells were then incubated with 5 μL APC Annexin V (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in their residual buffer before being incubated with anti-CD16/CD32 (‘Fc Block’) to reduce non-specific binding to Fc receptors (Biolegend). After 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... and NMHC-IIB antibody (#909901) binding to residues 1965-1976 in the C terminus (1:100 IF, 1:400 WB) were purchased from BioLegend. Rabbit polyclonal antibody against GFP tag (#50430-2-AP ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Biochemistry 2020Quote: ... Cells at a density of 2×107 cells/mL were incubated for 1 hour in flow cytometry buffer with 5 μL of Pacific Blue™ conjugated anti-CD235a antibody (BioLegend #349108) and 4 μg of unconjugated mouse IgG2a (Merck #M5409 ...