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Citations for BioLegend :
201 - 250 of 4729 citations for Recombinant Mouse Plaur Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... HA-Flag-tagged and GFP-tagged proteins were detected using HRP-conjugated monoclonal anti-HA (1:5000, BioLegend #901501) or polyclonal anti-GFP antibodies (1:2000 ...
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bioRxiv - Bioengineering 2023Quote: ... APC-conjugated His-tag antibody (Biolegend) was added to the cells treated with no nanobody proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-His secondary antibody (Biolegend, #362607) was used to detect IgG4-His primary antibodies ...
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bioRxiv - Immunology 2019Quote: ... recombinant murine SCF (BioLegend), recombinant murine G-CSF (BioLegend) ...
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bioRxiv - Bioengineering 2024Quote: ... Recombinant human FcγIIa (Biolegend), FcγRIIb (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Cancer Biology 2022Quote: RAW 264.7 cells or BMDMs were cultured as described above and then treated with recombinant mouse TGFβ1 (Biolegend). Fifteen minutes later ...
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bioRxiv - Immunology 2021Quote: Mice were injected intravenously under isoflurane anesthesia once daily with 1×105 U recombinant mouse IFNγ (575306, Biolegend) in 150 μL PBS ...
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bioRxiv - Immunology 2020Quote: ... The skin sheets were then transferred into another 1ml medium containing 0.1 μg of recombinant mouse 6Ckine (BioLegend) to enhance DC-migration ...
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bioRxiv - Immunology 2023Quote: ... freshly sorted cells were stained ex vivo or after stimulation with carrier-free recombinant mouse IL-25 (Biolegend) at a concentration of 100 ng/ml in complete RPMI medium for 36 h ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were blocked using an Fc blocking antibody (Biolegend Fc-block clone: 2.4G2) and lymphocytes were stained with CD3 and CD4 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... for 15 mins at room temperature and Fc blocked (2.5% human Fc block, BioLegend) for a further 10 mins at room temperature ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Immunology 2020Quote: Non-specific binding of antibodies to Fc-receptors was blocked with anti-mouse CD16/CD32 (TruStain fcX from BioLegend) in single cell suspensions prepared from Spl ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc receptors were blocked by incubating cells with TruStain FcX™ PLUS anti-mouse CD16/32 antibody (Biolegend, USA) for 10 min ...
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bioRxiv - Bioengineering 2020Quote: ... and treated with Fc-receptor blocker (2.5 μ g/mL, anti-mouse CD16/32; BioLegend, Cat# 101301, RRID: AB_312800) for 20 minutes at 4 °C in the dark ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with Fc Block (BioLegend) then with fluorochrome-conjugated antibodies (BioLegend unless noted ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG Fc (Biolegend; M1310G05), anti-CD27 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocking reagent (Biolegend, 422301) was used to block Fc receptor-introduced non-specific staining ...
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bioRxiv - Microbiology 2022Quote: ... CD16/32 (Fc block-Biolegend), B220 (AF700-Biolegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptor blockade (Biolegend 101319), and anti-CD31 antibody (BD Biosciences 561073) ...
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bioRxiv - Genomics 2024Quote: ... Fc-blocking reagent (BioLegend, 422301) was used to block Fc receptor-introduced nonspecific staining ...
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bioRxiv - Cell Biology 2023Quote: ... or by consecutive cell sorting for the expression of fluorescent proteins (mCherry-tagged or mVenus-tagged constructs) or the re-expression of TLR2 at the cell surface (Clone T2.5, Biolegend 121810) until the cell population was homogeneous ...
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bioRxiv - Immunology 2022Quote: ... and tetramerized using fluorescently tagged streptavidin from Biolegend. 5,6-Dimethylxanthenone-4-acetic Acid (DMXAA ...
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bioRxiv - Immunology 2021Quote: ... metal-tagged antibodies including pan-keratin (C11, Biolegend), BDCA2 (Polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... Fc receptors were blocked with 0.5% anti-CD16/32 Fc block antibody (Biolegend, clone 93) diluted in PBS containing 2% goat serum (Vector Laboratories ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the in vivo samples to obtain absolute counts of events acquired on the cytometer as well as using human and mouse Fc blocking antibodies (Biolegend) to reduce non-specific antibody staining ...
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bioRxiv - Bioengineering 2022Quote: ... and Phycoerythrin-conjugated Goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody (Fisher) for ACE2-Bite and APC anti-human IgG Fc Antibody clone HP6017 (Biolegend) for ACE2-Fc were added to the wells and incubated for another hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Surface staining was performed with anti-mouse Fc receptor antibody (clone 2.4G2, UCSF Hybridoma Core) or Human TruStain FcX (BioLegend #422302) in PBS with 2% FCS for 30 min on ice ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... Resulting single cell suspensions of splenocytes or bone marrow cells were Fc blocked with Trustain FcX (anti-mouse CD16/32 clone 93-Biolegend) and stained with combinations of the following antibodies ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of PE-conjugated mouse anti-human IgG Fc antibody (BioLegend) on ice in dark for 1h ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were first blocked with 2% mouse serum (eBioscience San Diego, California) and Human Fc Block (Biolegend San Diego, California) for 10 minutes on ice ...