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Citations for BioLegend :
201 - 250 of 308 citations for Mycoplasma Agar 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... 1ug/ml) and plate bound anti-CD3 (500 ug/ml) in the presence of Brefeldin A (1:1000, Biolegend) for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2024Quote: ... Optical densities (ODs) were read at 450 nm wavelength using a BioLegend Mini ELISA Plate Reader (BioLegend, Cat# 423555). Samples and standards were analyzed following subtraction of blank wells (1x PBS ...
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bioRxiv - Immunology 2024Quote: ... or 96-well plates precoated overnight at 4°C with 2 µg/ml anti-CD3ε antibody (145-2C11, BioLegend). For Th0 and Th17 differentiation ...
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio at 37C with 5% CO2 in a U-bottom 96-well plate in 200µL of T cell media with 10µL of anti-mouse CD107a-BV421 (BioLegend 121617). After 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to a 96-well plate and stained with zombie red (BioLegend, 1:1000 dilution in FACS wash buffer) for 30 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2021Quote: 96-well round-bottom plates were coated with either 2.5 µg/mL anti-CD3 (Biolegend, clone 145-2C11,Cat# 100359) or HIgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... splenocytes from OT-II/Rag1/CD45.1 mice were cultured in a-CD28 coated plates (Biolegend, Cat. No. 102115, 1ug/ml) with complete RPMI (10%FBS ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 1×105 TILs were seeded per well of a round bottom 96 well plate and stimulated with phorbol-12-myristate-13-acetate/Ionomycin in the presence of Brefeldin A (Biolegend) for 4 hours as per manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... The cells were activated in 96-well round bottom plates that were coated with 3ul/ml anti-CD3e (BioLegend #100202) and 5ul/ml anti-CD28 (BioLegend #102102 ...
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bioRxiv - Immunology 2021Quote: ... 0.05 mM β-mercaptoethanol) on a 96-well flat bottom plate coated with anti-CD3 antibody (10µg/ml, cat. # 317325, BioLegend). 7 x 103 DC3 or 5 x 104 monocytes were used per well ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to a non-CD3 coated plate and incubated for an additional three days before activation with Cell Activation cocktail with Brefeldin A (Biolegend) for 6 h and collection ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed four times with PBS-T and developed with 100 µL/well TMB High Sensitivity substrate solution (BioLegend, UK). After 5 to 10 min the reaction was stopped with 100 µL 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor cell lines were plated in triplicate on 96-well plates and treated for 72 hours with 10 ng/mL TNF (Biolegend) or 0.1% BSA vehicle control ...
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bioRxiv - Cell Biology 2021Quote: ... About 0.3 million cells in 37.5 μl CSM were loaded in each well of a 96-well plate of the Human Cell Surface Marker Screening (phycoerythrin [PE]) Kit (Biolegend). Each well contained 12.5 μl of diluted PE-conjugated antibody in CSM ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB High Sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... The plates were washed three times with PBS and developed with 100 µl/well TMB high sensitivity substrate solution (Biolegend). After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Cell Biology 2024Quote: ... Suspensions were transferred to a round-bottomed 96-well plate and were resuspended in a cocktail consisting of an Alexa Fluor (AF) 647-conjugated anti-GFP antibody (BioLegend) and a eFluor 780 fixable viability dye diluted in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... the appropriate number of wells of a flat-bottomed tissue culture plate were coated with 10 μg/mL αCD3 (Biolegend) in PBS for 3h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Nucleocapsid level on exosomes was measured by ELISA using precoated ELISA plates (Legend Max SARS-CoV2 Nucleocapsid protein ELISA Kit, 448007, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfected cells were then stimulated for 24h at 37°C with plate bound 1μg/ml of anti-CD3 (clone UCHT1, BioLegend #300402) and 2μg/ml of anti-CD28 (clone CD28.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were activated in 96-wells culture plates (25 x103 cells/well) coated with anti-CD3 and anti-CD28 (Biolegend) or by the addition of the DPP-8/9 inhibitor Talabostat (Val-boroPro ...
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bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...