6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine citations for BioLegend

Quote: ... B cells were stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...

Interpretable deep learning reveals the sequence rules of Hippo signaling

Khyati Dalal, et al., bioRxiv - Genomics 2024

Quote: ... at a 1:300 dilution with DAPI 2 μl in 1 ml of 10 μg/ml (BioLegend) and kept on light rotation for 2 h at RT ...

Sodium chloride in the tumor microenvironment enhances T-cell metabolic fitness and cytotoxicity

Dominik Soll, et al., bioRxiv - Immunology 2023

Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...

Early detection of cerebrovascular pathology and protective antiviral immunity by MRI

Li Liu, et al., bioRxiv - Neuroscience 2021

Quote: ... Alexa Fluor 488 anti-CD54 antibody (2 μg/mL, ICAM-1, YN1/1.7.4, BioLegend), and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL ...

Tumor genotype dictates radiosensitization after Atm deletion in brainstem gliomas

Katherine M. Deland, et al., bioRxiv - Cancer Biology 2020

Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...

Neutrophils require SKAP2 for reactive oxygen species production following C-type lectin and Candida stimulation

Giang T. Nguyen, et al., bioRxiv - Immunology 2021

Quote: ... and Dectin-2 expression were examined by using rat anti-mouse Dectin-1 (Biolegend) followed by Alexa-594-conjugated goat anti-rat (Invitrogen) ...

Endothelial Caveolin-1 and CXCL10 promote transcellular migration of autoreactive T cells across the blood-brain barrier

Troy N. Trevino, et al., bioRxiv - Immunology 2022

Quote: ... media was changed to complete EGM-2 with 1 ng/mL TNFα (Biolegend 575206) and shear stress of 1 dyn/cm2 was incorporated using a microfluidic pump (Ibidi 10902 ...

Decoding morphogen patterning of human neural organoids with a multiplexed single-cell transcriptomic screen

Fátima Sanchís-Calleja, et al., bioRxiv - Developmental Biology 2024

Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...

Lung-resident memory B cells maintain allergic IgE responses in the respiratory tract

Alexander J. Nelson, et al., bioRxiv - Immunology 2024

Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...

SARS-CoV-2 modulates NK cell responses via induction of HLA-E and triggers expansion of adaptive NK cells during acute infection

Mohammad Zahidul Hasan, et al., bioRxiv - Immunology 2024

Quote: ... Purified mouse anti-SARS-CoV-2 spike protein S2 antibody (1:3000 dilution, BioLegend) and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution ...

RIPK3 promotes brain region-specific interferon signaling and restriction of tick-borne flavivirus infection

Marissa Lindman, et al., bioRxiv - Immunology 2023

Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with fluorescently conjugated antibodies to CD3e (Biolegend ...

Multiomic Single Cell Sequencing Identifies Stemlike Nature of Mixed Phenotype Acute Leukemia and Provides Novel Risk Stratification

Cheryl A. C. Peretz, et al., bioRxiv - Cancer Biology 2023

Quote: ... and 1-2 million cells were incubated in 45ul of Cell Staining Buffer (BioLegend) per million cells with Trustain FcX block (BioLegend ...

Astrocytic RIPK3 exerts protective anti-inflammatory activity during viral encephalitis via induction of serpin protease inhibitors

Marissa Lindman, et al., bioRxiv - Immunology 2024

Quote: ... Leukocytes were blocked with 2% normal mouse serum and 1% FcX Block (BioLegend, #101320) in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend ...

Surface-associated antigen induces B-cell permeabilization and lysosome exocytosis facilitating antigen uptake and presentation to T-cells

Fernando Y. Maeda, et al., bioRxiv - Cell Biology 2021

Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).

State-dependent protein-protein interactions mediating 4-1BB CAR Signaling

Samuel A. Ritmeester-Loy, et al., bioRxiv - Immunology 2022

Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...

Inosine Induces Stemness Features in CAR T cells and Enhances Potency

Dorota D. Klysz, et al., bioRxiv - Immunology 2023

Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.

HOIL1 regulates group 3 innate lymphoid cell numbers in the colon and protects against systemic dissemination, colonic ulceration, and lethality from Citrobacter rodentium infection

Victoria L. Hartley, et al., bioRxiv - Immunology 2023

Quote: ... The cells were resuspended in 1 ml R10 and 1-2 million cells were plated in 1ml R10 with 1 μl Brefeldin A (BioLegend) for 4 hours ...

Single-Cell Profiling of the Antigen-Specific Response to BNT162b2 SARS-CoV-2 RNA Vaccine

Kevin J. Kramer, et al., bioRxiv - Immunology 2021

Quote: ... Bcl-6 FITC (Biolegend 358513, 1:100), and Glut-1 AlexaFluor 647 (Abcam ab115730 ...

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