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Citations for BioLegend :
201 - 250 of 6976 citations for 6 1 Aminoethyl 2H 1 4 benzoxazin 3 4H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 min at 4°C in the dark with annexin V-FITC or - pacific blue (1:100, Biolegend), near-IR fixable viability dye (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... Slides were then blocked and incubated overnight at 4°C with anti-Lysozyme antibody diluted 1:400 (Biolegend, #860001). Slides were washed and incubated with anti-rabbit Alexa Fluor 555 diluted 1:500 (Invitrogen ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Immunology 2019Quote: ... IL-6 was evaluated using the IL-6 DIY ELISA (Biolegend). ELISA was performed as per manufacturers instructions.
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bioRxiv - Immunology 2021Quote: ... cells were incubated with brefeldin A 2h at 37°C before surface stain with a mix of CD107a (LAMP1) [clone 1D4B (BioLegend)] and CD107b (Mac-3 ...
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bioRxiv - Immunology 2022Quote: ... Sca-1-PerCP-Cy5.5 (Biolegend, 1:1,000), CD45-APC-Cy7 (Biolegend,1:200) ...
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bioRxiv - Physiology 2022Quote: ... Mac-1 and Gr-1 (Biolegend, 133307), CD4 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... PD-1(Biolegend, RMP1-30, 1:200), KLRG1(Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Icam-1 Antibody (Biolegend, 116101, 1:60), Anti-Cytokeratin19 (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... Sigma)for one hour at room temperature and incubated overnight at 4 °C with the following primary antibodies: anti-CD63 (BioLegend, San Diego, CA, USA), anti-Cytochrome c ...
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bioRxiv - Cancer Biology 2022Quote: ... CD106 (1:100, MVCAM.A, #105717), CD62L (1:200, MEL-14, #104435), PD-1 (1:100, 29F.1A12, #135215) (Biolegend); Streptavidin (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1:300), NK1.1 (108736, 1:150), Tbet (644810, 1:50), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, CD19 (45-0193-82 ...
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bioRxiv - Immunology 2023Quote: ... 1:100), GR1 (108428, 1:300), NK1.1 (108728, 1:200), TCRβ (109243, 1:100), TER119 (116228, 1:200) from Biolegend, IL-22 (12-7221-82 ...
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bioRxiv - Cancer Biology 2020Quote: ... VDR overexpressing SKOV-3 and ECC-1 cells were processed and stained with PDL1-BV605 antibody (Biolegend Inc, Catalog number:124321). VDR expressing SKOV-3 and ECC-1 cells ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were blocked with PBS containing 3% BSA and stained with either APC-conjugate anti-human CD99 antibody clone 3B2/TA8 (1:20 dilution, BioLegend 341307), PE anti-human CD166 clone 3A6 (1:20 dilution ...
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bioRxiv - Genetics 2023Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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bioRxiv - Microbiology 2021Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Immunology 2021Quote: ... The sorted fraction was sequentially stained for 20 min at 4 °C with the Ly51-PE antibody (1:800) (Biolegend) and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
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bioRxiv - Immunology 2021Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer or peptide probes preincubated with streptavidin (30 minutes at molar ratio of 1:4 at 25°C) conjugated to PE (405203, BioLegend), BV421 (405226 ...
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cells from each individual mouse were stained using BioLegend TotalSeq B hashtags (Hashtags 1-4) and an anti-PE oligonucleotide barcode (BioLegend). Cells from each compartment (i.e ...
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...