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Citations for BioLegend :
2401 - 2450 of 4492 citations for 2 4 6 Trichlorophenol 13C6 99% 100 Ug Ml In Isooctane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and stained for 5 min at 4°C with biotinylated anti-CD3 antibody (17A2; BioLegend) to exclude CD3-positive T cells in the cell suspension using anti-biotin microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4°C overnight pERK antibody (1:20 dilution, Biolegend Cat. #675504). Samples were washed and resuspended for analysis ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... and then stained for 45 min at 4°C with the following antibodies from BioLegend diluted in FACS buffer (PBS with 2% FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 20 min in 150 μl 4% paraformaldehyde (BioLegend; cat. no., 420801) at 4 °C in darkness before a 30-min permeabilisation step (eBioscience ...
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bioRxiv - Immunology 2023Quote: ... tissue sections were stained O/N 4°C with either anti-CD11c-biotin (N418, Biolegend), anti-CD63 (NVG-2 ...
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bioRxiv - Immunology 2024Quote: ... and incubated overnight at 4°C with mouse anti-Aβ1-16 antibody (Biolegend, clone 6E10). Secondary antibody incubation was carried out for 2 hours at RT using goat anti-mouse IgG H+L – HRP (Jackson) ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Immunology 2019Quote: Polyclonal T cell activation was performed by stimulating healthy donor PBMCs at a concentration of 5×106 cells/mL with 0.1 μg/mL αCD3 (Clone OKT3, Biolegend 317315) and 1 μg/mL αCD28 (Clone CD28.2 ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded at a density of 2.5 × 104/well in 96-well plates pre-coated with 1ug/ml CD3 (Biolegend100339) and 1ug/ml CD28 (Biolegend 102115) antibodies and cultured in 1640 media with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Effector function was assessed by stimulating cells for 4 hours with PMA (20 ng/ml) and Ionomycin (1 μg/ml) with GolgiStopTM and monitored for CD8-PerCP (RPA-T8, Biolegend), CD4-AF700 (RPA-T4 ...
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bioRxiv - Immunology 2019Quote: ... isolated lymphocytes were first restimulated with 5 ng/ml phorbal 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin in the presence of monensin (Biolegend) for 3.5 hours ...
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bioRxiv - Bioengineering 2022Quote: ... Cytokine-primed BMDMs were cultured in RPMI growth media + 20 ng/mL M-CSF + 20 ng/mL IFNγ (BioLegend 575302) or 20 ng/mL IL-4 (BioLegend 574302 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 µL/well of either 10 µg/mL (flat-bottom) or 5 µg/mL (round-bottom) anti-mouse CD3ε (BioLegend) in PBS (for stimulated wells) ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Bioengineering 2022Quote: ... were modified by mixing a solution of nanovials in washing buffer with a cocktail of 40 µg/mL streptavidin and 20 µg/mL TotalSeqC conjugated streptavidin (Biolegend) solution at equal volumes ...
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bioRxiv - Cancer Biology 2022Quote: ... and activated using plate-bound anti-CD3 (1μg/ml; clone-145-2C11) and anti-CD28 (5μg/ml; clone-37.51) antibodies (Biolegend; San Diego, CA) for 4 days.
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bioRxiv - Immunology 2022Quote: ... isolated lymphocytes were first restimulated with 5 ng/mL phorbal 12-myristate 13-acetate and 500 ng/mL ionomycin in the presence of monensin (Biolegend) for 3.5 h ...
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bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with the following antibodies:(CEACAM6-FITC at 10 μg/mL, Sino Biological 10823-R408R; H2Kb-Pac Blue at 0.5 mg/mL, BioLegend 116514 ...
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bioRxiv - Immunology 2023Quote: ... along with co-stimulatory antibodies (CD28 and CD49d at 1 μL/mL) and Brefeldin-A (1 μL/mL, BioLegend, USA). LIVE/DEAD and surface staining was performed as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Cancer Biology 2024Quote: ... PCLS were stained in 250 μl of 5 μg/ml Hoechst 33342 and 2.5 μg/ml AlexFluor 647-conjugated anti-EpCAM (Biolegend 118212) by incubating for 1 hr at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF ...
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bioRxiv - Immunology 2024Quote: ... CTLs were seeded on sapphire discs coated with poly-L-ornithine (0.1 mg/ml) and anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402) in flat specimen carriers (Leica) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Genetics 2021Quote: ... cells were stained with anti-CD11b PE/Cy7 (1:100, Biolegend; 101206, M1/70) and anti-CD45 Alexa Fluor 647 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD86 was detected using rat anti-mouse CD86 (dilution 1/100, BioLegend, cat. 105001) and Alexa Fluor® 546 goat anti-rat secondary antibody (dilution 1/250) ...
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bioRxiv - Cancer Biology 2022Quote: ... PDL1 was detected using rat anti-mouse PDL1 (dilution 1/100, BioLegend, cat. 124302) as primary antibody and Alexa Fluor® 647 goat anti-rat (dilution 1/200 ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: All antibodies for immunofluorescence stain are as follow: FITC-LAMP1 (121605, BioLegend, 1:100), WIPI2 (SAB4200399 ...
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bioRxiv - Immunology 2021Quote: ... BV605 anti-human HLA-DR (clone L243; dilution 1:100; Biolegend; Cat no 307640); BV785 anti-human CD38 (clone HIT2 ...
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bioRxiv - Immunology 2021Quote: ... FITC anti-human CD8a (clone RPA-T8; dilution 1:100; Biolegend; Cat no 301050); Alexa Fluor 647 anti-human CD137 (clone 4B4-1 ...
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bioRxiv - Immunology 2021Quote: ... Primary antibodies (1:100): αCD3 (17A2) and αCD45 (30-F11) were purchased from BioLegend, insulin (Catalog # ...
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bioRxiv - Immunology 2021Quote: ... then Fixation buffer was added (100 μL, Cat: 420801, BioLegend, San Diego, CA, USA), and incubated for 20 min ...
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bioRxiv - Genomics 2021Quote: ... and resuspended in 100 μL of PBS containing Zombie aqua fixable viability dye (Biolegend) at 1:1000 ratio ...
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bioRxiv - Immunology 2021Quote: ... Tfh cells were labeled using anti-CD4 antibody (BioLegend, catalog no. 100402, 1:100) followed by anti-rat secondary antibody conjugated with Alexa Fluor 488 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... PE or APC mouse IgG1(1/100, MOPC-21, BioLegend, 400112 and 400120 respectively) were used to quantify CD62p exposure ...
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bioRxiv - Molecular Biology 2021Quote: ... Collected cells were re-suspended in 100 µL Red Cell Lysis Buffer (BioLegend, USA) and incubated for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell staining with 1:100 live/dead zombie yellow was first carried out (Biolegend) in PBS at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... density gradient centrifugation and then stained with PE anti-CD3 (1:100, BioLegend, 300308), PE-Cy7 anti-CD19 (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were further labelled with PE/Cy7-conjugated CD150 (TC15-12F12.2; Biolegend; 1:100) and APC/Cy7-conjugated CD48 (HM48-1 ...