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Citations for BioLegend :
2251 - 2300 of 8491 citations for 6 4 METHOXY PHENYL 2 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... washed with 1 mL FACS buffer and incubated with 1 μg mL-1 PE-anti HA.11 (BioLegend) at 4 °C with rocking for 1 h ...
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bioRxiv - Developmental Biology 2024Quote: ... 1:100) and Alexa Fluor 488 anti-mouse Ly-6A/E (Sca-1) antibody (108115, BioLegend, 1:1000) diluted in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 720-IC-200) or 1 μg/ml Fc-VCAM-1 (Recombinant Human VCAM-1-Fc Chimera, Biolegend, 553706) was added to the chamber slide ...
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bioRxiv - Immunology 2021Quote: ... mouse ICAM-1-Fc and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1-Fc was from R&D Systems (Minneapolis ...
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bioRxiv - Bioengineering 2022Quote: ... GFAP (rabbit, Dako Z0334, 1:1000; mouse, Biolegend SM121, 1:500). For fluorescent stainings ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mouse ICAM-1 and VCAM-1-Fc were from BioLegend. Recombinant mouse MAdCAM-1 was from R&D Systems.
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bioRxiv - Cell Biology 2021Quote: ... Mouse anti-HA (901501, WB: 1:2500; IF: 1:100; Biolegend).
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bioRxiv - Biochemistry 2020Quote: ... Ly-6A/E (Sca-1) (Biolegend, clone D7, BV605, 1:100), NK1.1 (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... and CD138 (Syndecan-1; 1:200; BioLegend, Cat. No. 142514; RRID:AB_2562198) were used in conjunction with the appropriate secondary antibody and visualized using the VECTASTAIN Elite ABC Reagent ...
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bioRxiv - Cell Biology 2022Quote: ... β-amyloid 1–16 (6E10) (Cat. 803001, BioLegend, 1:1,000, RRID:AB_2564653), Tubulin (Cat ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4), monoclonal mouse anti-mouse GFAP (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-APP 6E10 (Abeta 1-16) (1:1000, Biolegend #803004); rabbit anti-PITRM1 (1.1000 ...
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bioRxiv - Microbiology 2019Quote: Primary antibodies used were: anti-HA (Biolegend, 1:200 −1:400), anti-PKG (1:1000 (19)) ...
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bioRxiv - Immunology 2020Quote: ... and PD-1 (anti-PD-1-APC, clone RMP1-30, BioLegend). CXCR5−PD-1− iNKT cells from antigen challenged mice were also sorted for comparison (NKTeff).
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bioRxiv - Immunology 2021Quote: ... anti-Ly6G (clone 1A8, 1:100 BioLegend; 1:200 BD Biosciences); anti-CD182 (clone SA044G4/clone SA045E1 ...
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bioRxiv - Immunology 2022Quote: ... α-PD-1 (BD Biosciences, 1:100, J43 or BioLegend, 29F.1A12), α-CXCR5 biotin (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... PAX6 (PRB-278P, BioLegend, 1:500; AF8150, R&D, 1:200), PHOX2B (AF4940 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse IgG1-anti-rat CD45 (OX-1, 1:100, #202201, Biolegend) to label CD45+ cells ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7 (Clone 29F.1A12, BioLegend 135216, 1:50), KLRG1-PE-dazzle (Clone 2F1/KLRG1 ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7 (Clone 29F.1A12, BioLegend 135216, 1:50), KLRG1-PE-dazzle (Clone 2F1/KLRG1 ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7 (Clone 29F.1A12, BioLegend 135216, 1:50), CD62L-Alexa 700 (Clone MEL-14 ...
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bioRxiv - Immunology 2023Quote: ... anti–ICAM-1 (1:200 dilution, BioLegend, no. 116120 or 116108), and anti–CD24 (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... CD8β-FITC (1:400) and CD4-BV605 (1:400) (all Biolegend), TCRβ-BV786 (1:200) ...
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bioRxiv - Neuroscience 2023Quote: ... anti-β-Amyloid 1-16 (Clone 6E10, 803014, 1:5000, BioLegend, CA ...
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bioRxiv - Immunology 2023Quote: ... PE/Cyanine5 anti-mouse CD86 antibody(1:200; Biolegend, GL-1), PE/Cyanine5 anti-mouse CD3ε antibody (1:200 ...
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bioRxiv - Cell Biology 2022Quote: rat anti-DYKDDDDK (L5) (BioLegend, WB 1:1000, IF 1:200); rat anti-HA (Roche ...
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bioRxiv - Neuroscience 2024Quote: ... The cells were treated with 1 µg/ml MAR-1 (Biolegend), 1 µg/ml Ba13 (Biolegend) ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell surface staining was done for 30 minutes at 4 degrees with the following antibodies (all from BioLegend, San Diego, CA): CD45 (30-F11) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Immunology 2022Quote: ... 5ug/mL) and anti-CD28 (clone 37.51; 2ug/mL) in the presence of IL-4 neutralizing antibody (11B11, BioLegend, 5ug/mL) for 18-20 hours before the addition of cytokines as follows ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were first blocked in PBS with 10% rat serum with gentle rotation in a cold room and then stained for 1h protected from light at 4°C with CD206-FITC (cat no. 141704, Biolegend®) + MHCII-PE (cat no ...
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bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-BV421 and TNFα-PE diluted in Perm-Wash buffer (Biolegend).
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...