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Citations for BioLegend :
2201 - 2250 of 3545 citations for IL 17A Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... For intracellular staining cells were either permeabilized with 0.5% Saponin or 0.1% Triton and stained for TNF-α and/or IL-6 (BioLegend). Cells were washed with 2% serum supplemented 1X PBS and subsequently fixed with 1% PFA ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE–separated proteins were transferred to PVDF membranes and immunoblotted with primary antibodies against IL-1α (503207, Biolegend), IL-1β (12426 ...
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bioRxiv - Immunology 2022Quote: ... in the presence of plate-bound anti-CD3 (1 μg/ml) and soluble anti-CD28 (0.5 μg/ml) supplemented or not with IL-27 (30 ng/ml, BioLegend). Proliferation of Tconv cells was determined by CellTrace Violet Dye dilution in FACS analysis ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Immunology 2024Quote: ... purified ILC2 isolated from IL-33-challenged mice were cultured overnight with 10μM TOFA in complete media supplemented with 20 ng/ml IL-7 (Biolegend). Cells were washed with assay medium supplemented with 10 mM glucose ...
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bioRxiv - Immunology 2023Quote: ... injected at 0.5 mg per mouse: rat anti-mouse CD210 (IL-10R, clone 1B1.3a, BioLegend, Cat# 112712, Lot# B360179,) and rat IgG1 ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... IL-1β (432604) and Cathepsin B (EA100429) enzyme-linked immunosorbent assay (ELISA) kits were from BioLegend (San Diego, CA) and Origene (Rockville ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Biochemistry 2024Quote: ... We determined the amounts of IL-2 in the culture supernatant of activated or naïve CD4+ T cells with an ELISA kit (BioLegend). We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Cells were stained using the anti-human monoclonal antibodies fluorophore-conjugated (Biolegend) Brilliant Violet 605™ anti-human CD45 (HI30) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-human CD326 PE (BioLegend; Cat#324206; used at 1:200), and mouse IgM anti-human HTII-280 (Terrace Biotech ...
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bioRxiv - Immunology 2021Quote: ... 100 μl anti-human IgG or IgM - horseradish peroxidase (HRP) conjugates (BioLegend), diluted 1:2500 and 1:5000 in dilution buffer ...
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bioRxiv - Immunology 2021Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD309 (KDR/VEG-FR2) (Biolegend, clone 7D4-6; 1:200); anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human FcER1 BV510 (1:100, clone AER-37, BioLegend, cat # 334626), Anti-human CD303 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD163 PE (1:50, clone GHI/61, BioLegend, cat # 333606), Anti-human CD49d PE Dazzle 594 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD56 APC Cy7 (1:50, clone HCD56, BioLegend, cat # 318332), Anti-human CD45 APC Cy7 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human PD-1 BV785 (1:50, clone EH12.2H7, BioLegend, cat # 329930), Anti-human CD45 BUV395 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD4 BV510 (1:100, clone RPA-T4, BioLegend, cat # 300546), Anti-human CD56 BV605 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human MerTK PE Cy7 (1:50, clone 590H11G1E3, BioLegend, cat # 367610), Anti-human TIGIT PE Cy 7 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD103 BV711 (1:100, clone Ber-ACT8, BioLegend, cat # 350222), Anti-human CD10 BV711 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Granzyme K PE (1:25, clone GM26E7, BioLegend, cat # 370512), Anti-human FOXP3 PE CF594 (1:25 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CXCR6 PerCP Cy5.5 (1:50, clone K041E5, BioLegend, cat # 356010), Anti-human CCR8 PE (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CXCR4 APC Cy7 (1:50, clone 12G5, BioLegend, cat # 306528), Anti-human CTLA4 APC Cy7 (1:25 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD8a BV650 (1:50, clone RPA-T8, BioLegend, cat # 301042), Anti-human CD326 BV650 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CTLA4 APC Cy7 (1:25, clone BNI3, BioLegend, cat # 369634), Anti-human CD56 APC Cy7 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human HLA-DR BV785 (1:50, clone L243, BioLegend, cat # 307642), Anti-human PD-1 BV785 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45 APC Cy7 (1:100, clone HI30, BioLegend, cat # 304014), Anti-human CD14 APC eF780 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD8a PerCP (1:50, clone RPA-T8, BioLegend, cat # 301030), Anti-human CXCR6 PerCP Cy5.5 (1:50 ...
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bioRxiv - Immunology 2019Quote: ... The cells were washed and resuspended with Human TruStain FcX (Biolegend; 422302) for five minutes before adding the primary surface staining cocktail for 20m ...
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bioRxiv - Immunology 2020Quote: ... Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody (Clone HCD56, Biolegend), anti-CAR antibody Goat F(ab’)2 anti-Human IgG (H+L)-Alexa Fluor 647 (Jackson) ...
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bioRxiv - Immunology 2019Quote: ... cells were pre-incubated with Human TruStain FcX™ (BioLegend, catalog 422301) to block Fc receptors ...
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bioRxiv - Immunology 2019Quote: ... anti-human CD39 conjugated to APC (clone A1, cat. no. 328210, BioLegend), anti-human PD-1 conjugated to APC/Cy7 (clone EH12.2H7 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained by anti-human HLA-A2 antibody (BB7.2, BioLegend, USA) with PE-labeled ...
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bioRxiv - Cell Biology 2019Quote: ... PE/Cy7-conjugated anti-human/mouse CD49f (ITGA6) (1:20; GoH3; Biolegend), PE/Cy7-conjugated anti-human CD24 (1:20 ...
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bioRxiv - Genomics 2021Quote: The Human Anti-Virus Response Panel (13-plex; BioLegend, San Diego, CA) was used to quantitate human plasma cytokines (IL-1β ...
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bioRxiv - Immunology 2021Quote: ... 106 PBMCs were stained with FITC anti-human HLA-A2 antibody (BioLegend) at 4 °C in the dark for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend, Cat# 103045). Cells were washed by adding 1 ml 0.2 % BSA/PBS and pelleted for 5 min at 2800 x g ...
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bioRxiv - Biophysics 2021Quote: ... and then incubated with biotinylated anti-human CD3 antibody (OKT3) (BioLegend # 317319) at 1 ug/mL for 35 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated with BV421-conjugated anti-human CD11b (Biolegend # 301324) and APC-conjugated anti-human PMN Ly6G/6C antibodies (Biolegend # 108412 ...
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bioRxiv - Neuroscience 2020Quote: ... or PerCP-Cy5.5-conjugated mouse monoclonal anti-human CD200 (all Biolegend, UK) for analysis by flow cytometry ...
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bioRxiv - Systems Biology 2020Quote: ... anti-human CD166 mouse IgG1 monoclonal antibody clone 3A6 (BioLegend, cat: 343902), anti-human CD220 mouse IgG2 monoclonal antibody ...
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bioRxiv - Immunology 2021Quote: ... APC/Fire 750 anti-human CD226 antibody (DNAM-1) (clone 11A8, BioLegend), APC/Fire 750 anti-human KLRG1 (MAFA ...