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Citations for BioLegend :
2201 - 2250 of 9316 citations for 8 11 Methano 10a 3 6a 1 propanyl 3 ylidene 8H indeno 2 1 b azocine 12 14 dione 5 acetyloxy 4 benzoyloxy dodecahydro 1 3 dimethyl 9 methylene 3R 4S 5R 6aR 6bR 8S 10aR 11R 11aS 15S 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBST and streptavidin-conjugated to horseradish peroxidase was added (1:2000 in PBS, BioLegend) for 30 minutes at RT ...
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bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Cancer Biology 2024Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 µM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with different primary antibodies (Rat-anti-MBP, Sigma-Aldrich MAB386, 1:1000, monoclonal; Rabbit-anti-GFAP, CiteAb Z0334, 1:1000, polyclonal; Mouse-anti-6E10 Amyloid-β, BioLegend SIG-39300, 1:1000, monoclonal) diluted in 200 μl 10% NDS and 0.4% Triton-X blocking medium ON at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated with the following primary antibodies overnight at 4 : mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302) + rabbit anti-GFAP (1:500 dilutio L gilent Dako cat ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Cancer Biology 2022Quote: ... The following antibodies were used in this study: Alexa Fluor 488 anti-human SSEA-4 (#330412, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408 ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Cell Biology 2022Quote: ... The digested tissue was resuspended in 100 μL of 4% FBS in PBS containing an FITC antibody to CD11b (1:100; BioLegend, 101206) and PE antibody to CD45(1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... and granzyme B (anti-Granzyme B Pacific Blue, Biolegend, # 515407) production using flow cytometry (% positive T-cells) ...
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bioRxiv - Cell Biology 2019Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... from BD biosciences and APC-Cy7 anti-CD10 (1/100, #312212) PE anti-CD117 (1/100, #313210) from Biolegend.
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bioRxiv - Cancer Biology 2021Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...
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bioRxiv - Bioengineering 2019Quote: ... and Pacific Blue™ anti-Ly-6G/Ly-6C (Gr-1) (1:70, clone RB6-8C5, Biolegend).
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bioRxiv - Neuroscience 2019Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...
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bioRxiv - Microbiology 2022Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... T cell proliferation was stimulated with anti-CD3ε (1 μg/mL)/CD28 (1 μg/mL) antibodies (BioLegend). The anti-CD3ε antibody was coated on each well of the plate via overnight incubation at 4°C with PBS before coculture ...
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bioRxiv - Immunology 2023Quote: ... 1 × 106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa-Fluor®-488 SSEA1 (CD15) (1:50) and Alexa-Fluor®-647 SSEA5 (1:200) (BioLegend). In all experiments ...
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bioRxiv - Immunology 2023Quote: ... ST2 and CD13 was verified using FITC-coupled anti-FcεRI (1:100, clone MAR-1, BioLegend, #134306), PE-coupled anti-KIT (1:100 ...
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bioRxiv - Immunology 2023Quote: ... CD40-APC (1:100) and CD11c-e450 (1:100) (all antibodies from Biolegend, San Diego, CA, USA). Cells were incubated with 100μl of the antibody suspension for 20 minutes at 4°C before a final wash in Ca2+/Mg2+ free PBS containing 2mM EDTA at x400g for 5 minutes at 4°C ...
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bioRxiv - Physiology 2024Quote: ... and immunofluorescence staining for PECAM-1 (AlexaFluor488 labelled primary monoclonal rat antibody, 5μg mL-1, MEC13.3, BioLegend) and S100A9 (Cy5.5 directly labelled ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies in 1:200 dilutions were used: CD31-PE-594 (1:200, Biolegend, Cat# 102520), CD45-APC (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with BV711 (1:50) or AlexaFluor647 (1:300) anti-mouse T-bet (Biolegend, 4B10), PE eFluor610 anti-mouse Eomes (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were incubated for 15 minutes at 4°C in streptavidin-PE/Cy7 (BioLegend) to detect NCAM-biotin ...
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bioRxiv - Immunology 2022Quote: ... CD115 APC (Biolegend, clone 9-4D2-1E4), CD45RA PE (BD Bioscience ...
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bioRxiv - Immunology 2022Quote: ... CD115 APC (Biolegend, clone 9-4D2-1E4), CD45RA PE (BD Bioscience ...
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bioRxiv - Immunology 2022Quote: ... CD115 APC (Biolegend, clone 9-4D2-1E4), CD116 AP-Vio770 (Miltenyi ...
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bioRxiv - Immunology 2022Quote: ... CD115 APC (Biolegend, clone 9-4D2-1E4), CD71 PE (BD Bioscience ...
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bioRxiv - Immunology 2022Quote: ... CD115 APC (Biolegend, clone 9-4D2-1E4), CD45RA PE (BD Bioscience ...
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bioRxiv - Immunology 2023Quote: ... 10µg/ml anti-gal-9 mAb (BioLegend) was added to the co-culture from the start of the assay.
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bioRxiv - Immunology 2021Quote: ... Granzyme B (BioLegend), LAMP1 (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained in FC buffer (2% FCS in PBS) with anti-CD3-APC conjugated antibodies (1:80; BioLegend) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000 ...