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Citations for BioLegend :
2201 - 2250 of 8294 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
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bioRxiv - Immunology 2022Quote: ... IL-2 and IL-7 were removed from ILC2 culture and additionally cultured for 4 hr before restimulation with IL-33 (Biolegend, #580508) plus NMU (R&D systems ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% paraformaldehyde (PFA) and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-BV421 and TNFα-PE diluted in Perm-Wash buffer (Biolegend).
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Immunology 2021Quote: ... PD-1 tetramers were prepared by incubating a 5:1 ratio of biotinylated PD-1 and PE streptavidin (BioLegend) for 10 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... 1-16 Antibody (1:000, Clone 6E10, N-terminal, a.a. 1-16, human specific only, 803014 BioLegend, CA, USA); β-Amyloid ...
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bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
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bioRxiv - Neuroscience 2020Quote: The cerebellar slices were treated either 3 times 24 hours apart with combinations of recombinant TNF (recombinant rat TNF, Biolegend, San Diego, CA, USA), LTα (recombinant human LT-α ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... half a million cells per sample were incubated for 10 min in 50 μl of PBS with 3% FSB and 5 μl of blocking buffer (BioLegend, San Diego, CA, USA) and then stained with 50x diluted TotalSeq-B cell feature barcode antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... spleen T cell IL-1β/IL-23 stimulation group (SPT stim) with hashtag 2 (BioLegend, Cat# 155803); spleen T cell IL-1β/IL-23 plus spermidine treatment group (SPT sper ...
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bioRxiv - Molecular Biology 2021Quote: ... or day 5 IL-2 cultured NK cells isolated using the MojoSort NK isolation kit from Biolegend, but instead of analysis by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 594 or Alexa Fluor 647 conjugated anti-mouse CD31 (2 μg/mL; clone MEC13.3; BioLegend) was used ...
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bioRxiv - Immunology 2022Quote: ... as well as SARS-CoV-2-S1 (biotin-coupled; Acro; #S1N-C82E8) and fluorochrome-coupled streptavidin (Biolegend). The anti-ST6GAL1 and anti-FUT8 Abs were labeled with Alexa Fluor 488 labeling kit (Life Technologies GmbH ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-centrifuged 50 μL of hashtag antibody solution containing 2 uL of TotalSeq-A Hashtag antibody (BioLegend), with a unique barcode sequence for each iPS cell line ...
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bioRxiv - Physiology 2022Quote: ... mice were intravenously injected with 2 μg of Alexa Fluor 647 anti-mouse CD127 antibody (A7R34, BioLegend) and euthanized 5 minutes after injection ...
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bioRxiv - Immunology 2022Quote: ... The abundance of Nectin-2+ monocytes and NK cell intracellular cytokine production of IFN-γ (4S.B3, Biolegend) were analyzed by flow cytometry (BD LSRFortessa™ Cell Analyzer ...
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bioRxiv - Immunology 2022Quote: Cells were stained for flow cytometry using the following antibodies diluted in FACS Buffer (2% FBS, 0.01% Sodium Azide, PBS): BV711-anti-CD326 (EpCAM) (Clone:G8.8, BioLegend), BUV395-anti-CD45.2 (Clone:104 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100,000–500,000 single cells of breast tumours were suspended in 100μl staining buffer (2% BSA, 0.01% Tween in PBS) before adding 10µl Fc-blocking reagent (FcX, BioLegend). and incubating during 10 min on ice ...
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bioRxiv - Immunology 2020Quote: ... anti-human BDCA-2-PE (clone AC144; Miltenyi Biotech) and anti-human CD123-APC (clone 6H6; Biolegend). Cells were incubated with surface antibodies at 4°C for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for IL-2 were treated with Cell Activation Cocktail with Brefeldin A (Biolegend, Cat #423304) for 4 hours prior to fixation ...
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bioRxiv - Immunology 2022Quote: ... B cells were stimulated for 2 days with 10 ug/ml of anti-CD40 (Biolegend, Cat#102812) and 10 ng/ml of rec ...
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bioRxiv - Immunology 2022Quote: ... in the presence of rhIL-2 (50U/ml, Novartis) and anti-IFNγ (5µg/ml, clone XMG1.2, Biolegend). For intracellular cytokine staining ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 2 times in 1x Perm/Wash buffer and resuspended in Cell Staining Buffer (Biolegend) prior to flow cytometry analysis ...
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bioRxiv - Immunology 2024Quote: ... and incubated for 2 hours after which cells were treated with 1x Brefeldin A (BioLegend; cat#: 420601) and incubated for a final 4 hours before analysis by flow cytometry for T cell activation.
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bioRxiv - Immunology 2024Quote: ... and penicillin in a 96-well plate pre-coated with anti-CD3Ab (2 μg/ml, Biolegend 100360), along with the addition of 5 μg/ml anti-CD28 antibody (Biolegend 102122 ...
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bioRxiv - Immunology 2023Quote: ... aliquots of conditioned media were collected for measuring IL-2 and IFNγ by ELISA (ELISA MAX, BioLegend). Cells were washed and stained with anti-mouse CD3ε-FITC (145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... serum samples were diluted 2-fold with assay buffer and standards were mixed with matrix solution (Biolegend) to account for additional components in the serum samples ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...