Labshake search
Citations for BioLegend :
2151 - 2200 of 2527 citations for 6H Furo 2 3 b pyrrole 5 carboxylicacid 6 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... for 5 minutes at room temperature prior to staining with a surface-stain cocktail containing the following antibodies purchased from BioLegend® (San Diego ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5-10 x 106 cells were washed with PBS and stained with Zombie NIR Fixable live/dead dye (Biolegend, 423106) at 4 °C for 20 min ...
-
bioRxiv - Immunology 2022Quote: ... 50,000 CD4+ T cells were added to each well of mBECs in 12-well chamber slides in Hank’s balanced salt solution (HBSS) with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
-
bioRxiv - Immunology 2022Quote: ... isolated lymphocytes were first restimulated with 5 ng/mL phorbal 12-myristate 13-acetate and 500 ng/mL ionomycin in the presence of monensin (Biolegend) for 3.5 h ...
-
bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
-
bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
-
bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
-
bioRxiv - Immunology 2024Quote: ... isolated naïve splenic B cells were cultured at a density of 0,5 x 106 cells/ml in the presence of 25 μg/ml LPS and 5 ng/ml IL-4 and stained with anti-CD138 (BioLegend). B cell proliferation was assessed by CellTrace Violet (Thermofisher ...
-
bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
-
bioRxiv - Immunology 2024Quote: ... Plates were washed 5 times in 150 µL of PBST and incubated with 100 µL of streptavidin conjugated to horseradish peroxidase (HRP, BioLegend) diluted 1:2000 in PBS for 1 hour at RT ...
-
bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBST and streptavidin-conjugated to horseradish peroxidase was added (1:2000 in PBS, BioLegend) for 30 minutes at RT ...
-
bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
-
bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
-
bioRxiv - Immunology 2023Quote: For Langerhans cells analysis, after centrifugation (450g, 5 min) cells were stained with anti-CD45 FITC (BioLegend, clone 30-F11), anti-EpCAM APCFire750 (BioLegend ...
-
bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... Pospho-GSK-3α/β and pospho-Akt were quantitated following 5 minutes of stimulation in transfected T cells fixed and permeabilized with fixation and permeabilization buffers (Biolegend) and stained with anti-pospho-GSK-3α/β (R&D systems ...
-
bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
-
bioRxiv - Immunology 2023Quote: ... anti-CD25(Percp-Cy5.5, #302626), anti-CD69(APC, #310910), anti-mouse CD25(PE, #12-0251-83), anti-mouse CD69(FITC, #104506) from Biolegend, anti-human IgG(SureLight® PE ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
-
bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
-
bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
-
bioRxiv - Immunology 2023Quote: ... lysis was performed at room temperature for 5 minutes in 1X RBC lysis buffer (10X RBC lysis buffer diluted in mqH2O, 420301, BioLegend) and reaction was stopped by diluting the 1X RBC lysis buffer with 35ml 1X DPBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with FACS buffer and pre-incubated with 5 μg/mL TruStain FcX anti-mouse CD16/32 antibody (clone 93, BioLegend) in 100 μL FACS buffer for 5 minutes on ice before immunostaining ...
-
bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
-
bioRxiv - Cancer Biology 2024Quote: ... PCLS were stained in 250 μl of 5 μg/ml Hoechst 33342 and 2.5 μg/ml AlexFluor 647-conjugated anti-EpCAM (Biolegend 118212) by incubating for 1 hr at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
-
bioRxiv - Immunology 2024Quote: ... T cell stimulations were performed by first coating a 96-well plate with 5 μg/mL anti-mouse CD3 (Biolegend) in 50 μL/well PBS overnight the day before tissue harvest ...
-
bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
-
bioRxiv - Bioengineering 2024Quote: ... for 5 min on ice and then stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat: 152406) CD95 (PE-Cy7 ...
-
bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatant was discarded and the pellet was resuspended in 100µl FACS Stain Buffer (2% FBS in PBS) with 1µl anti-CD45-APC/Cy7 (clone 30-F11, BioLegend) and 1µl anti-EPCAM-AlexaFluor488 (clone G8.8 ...
-
bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Digested meniscus cells were re-suspended in flow buffer (2% FBS/PBS) and stained with Sca1 (BioLegend, 108131), Cd90 (BioLegend ...
-
bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
-
bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
-
bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
-
bioRxiv - Synthetic Biology 2019Quote: ... supplemented with 2% FBS and stained with FITC-AnnexinV/PI Apoptosis Detection Kit according to manufacturer’s instructions (BioLegend). Samples were then run on a LSRFortessa X-20 flow cytometer (BD Biosciences ...
-
bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-Vimentin (Santa-Cruz; 2 μg/mL) and mouse anti-placental alkaline phosphatase (Biolegend; 1 μg/mL). Immunostaining for hCMV was performed as previously described [32] ...
-
bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
-
bioRxiv - Immunology 2022Quote: Whole blood inoculated with SARS-CoV-2 was blocked with 5μl of TruStain (Biolegend, San Diego, California, # 422302) for 10 minutes at room temperature and then stained for granulocyte and monocyte markers ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
-
bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.