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Citations for BioLegend :
2101 - 2150 of 2728 citations for Recombinant Human IL12A His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... T cells were isolated and stained with fluorochrome-labeled anti human CD3 (BioLegend, Cat. No. 317342) and anti human CD8a (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC) (BioLegend; Cat. No. 334112) for 30 minutes in the dark ...
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bioRxiv - Immunology 2023Quote: Zombie Aqua Fixable Viability Dye and Human TruStain FcX Fc Receptor Blocking Solution were from BioLegend. CF700-SE and CF750-SE were bought from Biotium ...
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bioRxiv - Cell Biology 2023Quote: ... and CF568-labeled antibodies specific for human CD9 (BioLegend, Cat. No. 312102, San Diego, CA, USA) and human CD81 (BioLegend ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were then stained for 30 minutes with: Human anti-CD3 (1:400; BioLegend, clone: SK7), human anti-CD4 (1:400 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained with 1:100 diluted anti-human CD66b-Pacific Blue (BioLegend, 305112). Cells were washed and bead uptake was measured on a BD LSR II (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Immunology 2023Quote: ... Adoptively transferred human PBMC were stained with the following antibodies: CD4-PerCP-Cy5.5 (BioLegend, clone SK3), CD8a-APC-Cy7 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... BV421™ anti-mouse/human CD45R/B220 Antibody (Clone RA3-6B2; BioLegend; Cat#103251; RRID: AB_2562905) BV605 Mouse Anti-Mouse CD45.1 (Clone A20 ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were stained with a biotinylated anti-human CD271 (NGFR) antibody (BioLegend, Cat# 345122). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, The Netherlands), APC anti-human CD206 (clone 19.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, the Netherlands), Brilliant Violet 450 anti-human CD3 (Clone UCHT1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were used directly for cytokine analysis with the LEGENDPlex Human Inflammatory Panel 1 (BioLegend, 740809) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cultures were treated with anti-human CD2 mAb (10µg/mL; Cat# 300240; Clone: RPA-2.10; Biolegend) or IgG (10 µg/mL ...
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bioRxiv - Immunology 2023Quote: Extracellular staining was performed using the following antibodies: anti-human CD3ε BV785 (1:50, OKT3, BioLegend), anti-human CD19 BV605 (1:20 ...
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bioRxiv - Microbiology 2023Quote: ... IL1beta ELISAs were performed using the ELISA MAX™ Deluxe Set Human IL1beta kit (437015, Biolegend) following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... and cytokine levels determined using the bead-based immunoassays LEGENDplex™ Human Macrophage/ Microglia Panel (Biolegend). The assays were performed in 96-well plates following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-human CD274 (B7-H1, PD-L1) (BioLegend, cat. no. 329707; 1:1000 for WB); mouse anti-DAAM1 (Santa Cruz Bio ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were stained with 5 ul each of PE-anti human CD45 antibody clone HI30 (Biolegend) and APC-anti human CD11b antibody clone ICRF44 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... All flow cytometry anti–human mouse Ab probes and ZombieGreenTM were purchased from Biolegend (CA, USA). UltraComp eBeadsTM Plus Compensation beads (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: Quantification of secreted cytokines was conducted using the 13-plex LEGENDplex Human Macrophage/Microglia panel (BioLegend) designed for the identification of IL-12p70 ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions were labelled with TotalSeqA anti-human hashtag oligonucleotide (HTO) antibodies (Biolegend, 1:50) to allow downstream de-multiplexing in silico as previously described9 ...
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bioRxiv - Cell Biology 2024Quote: ... or 2.5 µl of fluorescein isothiocyanate (FITC)-conjugated anti-human CD41/CD61 (clone PAC-1, BioLegend) for 2 h at room temperature (RT ...
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bioRxiv - Biophysics 2024Quote: ... PBMCs for microscopy measurements of monocytes were incubated with Alexa Fluor 647 anti-human CD14 (Biolegend) following the protocol described above and stained with Pro12A for GP quantification.
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Genomics 2024Quote: ... and stained with the TotalSeq-A Human Universal Cocktail panel according to the manufacturer’s instructions (BioLegend).
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bioRxiv - Developmental Biology 2023Quote: ... and incubated with the monoclonal anti-human antibodies anti-CD56-PE and anti-CD82-Alexa647 (BioLegend). After 30 minutes incubation on ice ...
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bioRxiv - Genomics 2024Quote: ... then resuspended in 105μL of cell staining buffer with 1:21 Human TruStain FcX (BioLegend 422301), followed by incubation at 4°C for 20 mins at 300rpm ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 50 mg/mL APC-mouse-anti-human CD56 (BioLegend; Cat. No. 318310; clone HCD56), 0.5 mL 200 mg/mL APC/Cy7-mouse-anti-human CD3 (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... we used the following anti-human antibodies for surface-marker staining: anti-CD45 (BV785, clone HI30 – BioLegend), anti-CD3 (Alexa700 ...
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bioRxiv - Cancer Biology 2022Quote: ... Western blot antibodies for the GAPDH loading control were mouse anti-human GAPDH antibody (Biolegend, catalog #649202) and HRP-conjugated anti-mouse IgG (Cell Signaling Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Purity was assessed by FACS staining with anti-human CD34 BV 421 antibody (clone 561, BioLegend, USA). For long-term storage ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor® 700 anti-human CD4 (clone RPA-T4; dilution 1:200; Biolegend; Cat no 344808), FITC anti-human CD8a (clone RPA-T8 ...
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bioRxiv - Microbiology 2019Quote: ... Sputum samples were also stained with 1 µg/ml of PE anti-human CD45RO in PBS (BioLegend) for 30 min to stain activated and memory T cells ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Bioengineering 2021Quote: ... and selected antibodies (APC-labeled mouse anti-human: CD45, CD90, CD105, CD309 and CD147, all from BioLegend) for 30 min in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were stained with 0.1ug TotalSeq-B anti-human Hashtag antibody (Biolegend, 394633, 394635, 394637 or 394639) per sample diluted in 100uL HF buffer for 30 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Raji B cells were stained for CD19 with PE anti-human CD19 antibody (Biolegend, #392506, clone 4G7) and the top 3% highest CD19-expressing cells (Raji B cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: Erythroid progression was followed by immunostaining with phycoerythrin (PE)–conjugated anti-human CD235a (1:100) (116207, BioLegend) and Allophycocyanin (APC)–conjugated anti-CD71 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: Samples were labelled and imaged with the following primary antibodies: anti-human CD41 (BioLegend Cat# 303702, RRID:AB_314372), anti-human CD34 (BioLegend Cat# 343502 ...
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bioRxiv - Cancer Biology 2022Quote: Flow cytometry analysis or flow cytometry cell sorting was performed using anti-human CD133-APC (BioLegend – 372805) and anti-human CD44-APC (BioLegend – 338805 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed once with PBS and incubated with anti-human antibodies against CD3 (PerCP-Cy5.5, Biolegend), CD56 (BV510 ...
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bioRxiv - Systems Biology 2022Quote: ... washed twice with PermBuffer and stained with anti-human γH2AX AlexaFluor 488 (Biolegend, clone 2F3, cat. #613405) for 1 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504] ...