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Citations for BioLegend :
2101 - 2150 of 2921 citations for Recombinant Human HFE2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Tim-3 was either neutralized by anti-human Tim-3 antibodies (10 µg/mL) (Biolegend) for 1 h prior to treatment or through knockdown via duplexed siRNA (Integrated DNA Technologies ...
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bioRxiv - Immunology 2020Quote: ... The cells were then stained with PE labeled anti-human IgG Fc secondary antibody (Biolegend) at a 1:20 dilution in 50 μl staining buffer at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended at 106 cells/mL and then treated with Human TruStain FcX blocker (BioLegend 422302). Cell sorting was carried out in azide-free buffers ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with 40 ng/ml human IL-10 (BioLegend, San Diego, CA, USA) for 2 h at 4°C and cells were collected for flow cytometric analysis.
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bioRxiv - Molecular Biology 2019Quote: ... and stained with anti-human-MHCI-APC (Biolegend, anti-HLA-ABC, clone W6/32, 311410). Cells differentiated at ALI were detached from transwells using StemPro Accutase Cell Dissociation Reagent (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... the cells were fixed and stained by anti-human mAb FOXP3-PE antibody (BioLegend, CA) and anti-human mAb CD4–FITC clone RPA-T4 (BioLegend ...
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bioRxiv - Immunology 2019Quote: ... 1mM EDTA and 0.1% sodium azide) and incubated with Human TruStain FcX™ (BioLegend, #422302) for 20min ...
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bioRxiv - Cancer Biology 2020Quote: ... Other cytokines were assayed using the LEGENDPlex multiplex assay (Human Anti-Virus Response Panel, BioLegend). Fluorescence was analyzed using a FACS Canto II (BD Biosciences).
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bioRxiv - Molecular Biology 2021Quote: ... The levels of bFGF were quantified with human bFGF ELISA kit (BioLegend, Cat. Nr. 434309) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of PE anti-human IgG Fc antibody (clone HP6017, BioLegend, Cat. No. 409304) buffer-exchanged into 1x PBS was added to yeast ...
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bioRxiv - Immunology 2021Quote: ... and then treated with Human TruStain FcX (Fc receptor blocking solution, Cat. No. 422302, BioLegend) for 5 mins ...
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bioRxiv - Genetics 2020Quote: ... Bone marrow cells were first incubated for 15 minutes with Human TruStain FcX (BioLegend #422302) and TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Cell Biology 2021Quote: ... Human conjugated antibodies used for assessing engraftment and differentiation derived from Biolegend (1:100 dilution); huCD45-Brv421 (368522) ...
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bioRxiv - Genetics 2021Quote: ... The cell suspension was incubated in Human TruStain FcX blocking solution (1:20, Biolegend; 422302) at room temperature for 10 minutes ...
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bioRxiv - Genomics 2020Quote: ... anti-human CD29 and anti-mouse CD29 antibodies were purchased from Biolegend (cat. 303021, 102235) and conjugated per antibody using a ThunderLink kit (Expedeon cat ...
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bioRxiv - Immunology 2020Quote: Human ILC2 cells were stained with 1 μM CSFE (CSFE cell division tracker kit, BioLegend) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... confluent BCR-expressing B cells were FACS sorted on IgM (APC anti-human IgM; BioLegend) and kappa light chain (PE anti-human kappa light chain ...
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bioRxiv - Immunology 2021Quote: ... and 1×106 cells were incubated sequentially or together with Human Trustain FcX (BioLegend #422302) and Fixable Viability Stain 510 (BD #564406) ...
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bioRxiv - Immunology 2020Quote: ... with 100ug of antibodies against specific human NK cells expressing NKp46 Domain 1 (Biolegend, 9E2) and/or human CD8α (Bioxcell ...
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bioRxiv - Immunology 2021Quote: ... Samples were thawed and proteins quantified using a LEGENDplex Human Proinflammatory Chemokine Panel immunoassay (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by addition of relevant detection mAbs: biotin anti-human IFN-γ (Clone: 4S.B3, BioLegend); biotin anti-human TNF-α (Clone ...
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bioRxiv - Genomics 2021Quote: ... and incubated with Human TruStain FcX™ (Fc Receptor Blocking Solution) (Biolegend, Cat#422302, USA) for 15 minutes on ice ...
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bioRxiv - Immunology 2021Quote: Cytokines expression was evaluated using the Human T Helper Cytokine Panel (Biolegend, San Diego, CA) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were used at 1:1000 dilution: mouse anti-human CD63 (clone H5C6, Biolegend); mouse anti-human CD81 (TAPA-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human CD3 Antibody (#300322, Mouse IgG2a κ, dilution 1:100, Biolegend); APC Rat IgG2a κ Isotype Ctrl (#400512 ...
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bioRxiv - Cell Biology 2022Quote: ... PE Rat anti-Mouse/Human CD44 (Monoclonal; clone IM7; Biolegend; 1:100; Cat#103007, FACS). BUV395 Rat anti-Mouse CD45 (Monoclonal ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with PBS and then incubated with Human TruStain FcX Blocking Solution (Biolegend) for 10 mins ...
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bioRxiv - Genomics 2022Quote: ... Ligand expression was confirmed for engineered 293T cells using anti-human CD28-PE (302907, Biolegend), anti-human CD20-PE (302306 ...
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bioRxiv - Immunology 2022Quote: ... Samples were thawed and used undiluted in LEGENDplex™ Human Anti-Virus Response Panel (Biolegend) according to manufacturer’s instructions to measure different cytokines ...
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bioRxiv - Immunology 2024Quote: ... and stimulated with plate bound anti-human CD3 Antibody (clone OKT3, 5ng/mL, #317326, Biolegend) and aCD28 (clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-human PE-Cy™7 CD27 (M-T271) (BioLegend, San Diego, CA, USA). Sorted TFH ...
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bioRxiv - Immunology 2022Quote: ... Nalm6 and primary B cells were labeled with PE/Cy7 anti-human CD19 Antibody (Biolegend) and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2 ...
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bioRxiv - Microbiology 2022Quote: ... a LegendPlex Human Anti-Virus Response Panel were used according to the manufacturer’s directions (BioLegend). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were analyzed by SDS-PAGE and western blot using total human Tau (#835201, Biolegend), 14-3-3 (#ab51129 ...
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bioRxiv - Bioengineering 2024Quote: ... plates were coated with 2Lμg/ml of a mouse anti-human CD4 antibody (BioLegend, 300502) diluted in PBS overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by human CD3 and CD8 (clone OKT3 and clone SK1, BioLegend, San Diego, CA) stain ...
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bioRxiv - Cancer Biology 2024Quote: ... The PE anti-human TGF-β Receptor II Antibody (clone W17055E, BioLegend, San Diego, CA) was used to detect dnTGFβRII expression on the CAR ...
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bioRxiv - Immunology 2024Quote: ... we used anti-human antibodies for surface-marker staining: anti-CD45 (BV785, clone HI30 – BioLegend), anti-CD3 (Alexa700 ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed and permeabilized cells were stained with PE anti-human Ki-67 antibody (BioLegend #350504) diluted 1:10 in the BD Perm/Wash buffer from the kit for 30 min on ice ...
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... CD69 levels were measured with APC-conjugated anti-human CD69 antibodies (Biolegend, 310909, clone FN50). TCR levels were measured with BV421-conjugated anti-human TCR antibodies (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was analyzed using the LEGENDPlex™ Assay (BioLegend; Human Inflammation Panel 1 (13-plex)) as per manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... and isotype controls (mouse IgG1κ, clone MG1-45, BioLegend or human IgG1κ, clone QA16A12, BioLegend) were used as controls ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with anti-human IgG-APC (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Surface marker staining was performed for 30min after 15min blocking with human TruStain FcX(BioLegend). Cells were washed in FACS buffer(PBS+2% FCS ...
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bioRxiv - Bioengineering 2023Quote: ... Human macrophages were stained with anti-CD11b (M1/70) and anti-CD64 (10.1) mAbs (Biolegend). Murine macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Bioengineering 2023Quote: ... The LEGENDplex™ Human Essential Immune Response Panel (13-plex) (BioLegend, San Diego, CA, USA) was employed to quantify the secreted cytokines in the cell culture supernatant following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 50µL ice-cold 1:10 human TruStain FcX (BioLegend 422302) in MACS buffer and incubated for at least 5 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... for 15 minutes and incubated with the human-specific anti-CD298-APC antibody (Biolegend, 341706) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was then stained with Alexa Fluor 488 anti-human CD3 antibody (BioLegend, 300320), PE anti-human CD25 antibody (BioLegend ...