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Citations for BioLegend :
2101 - 2150 of 2773 citations for Rat Interleukin 10 Receptor Beta IL10Rb ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions were stained with Zombie UV fixable viability kit (Biolegend), FITC-conjugated Hematopoietic Lineage cocktail prepared in-house (CD2 (TS1/8) ...
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bioRxiv - Immunology 2023Quote: After Fc blocking and Live/Dead staining (Zombie Fixable Viability Kit, Biolegend), extracellular staining was done in ice cold phenol red-free DMEM containing 2% FBS for 30 min using antibodies against surface markers (see antibodies below) ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using Zombie NIR™ Fixable Viability Kit (BioLegend). For detection of EdU incorporation ...
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bioRxiv - Microbiology 2023Quote: ... FITC Annexin V Apoptosis Detection and Zombie Red Fixable Viability kits (Biolegend) were used following the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Tissue clearing was performed using Ce3D™ Tissue Clearing kit (427702, Biolegend) according to manufacturer instructions with modifications ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained for viability with Zombie UV Fixable Viability Kit (Biolegend) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were stained with Zombie NIR™ Fixable Viability Kit (BioLegend) according to the manufacturer’s instructions and then detached using trypsin ...
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bioRxiv - Immunology 2024Quote: ... we used mouse-specific CD8 T cell negative isolation “MojoSort” kits (BioLegend). We plated 0.5 to 1 x 106 CD8 T cells per well in 96-well V-bottom tissue culture plates ...
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bioRxiv - Microbiology 2024Quote: ... Zombie Violet Fixable Viability kit was bought from Biolegend (cat no. 423114). ZymosanA-Alexa Fluor™ 594 ...
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bioRxiv - Immunology 2024Quote: ... viability was measured using the Deep Blue Cell Viability Kit (Biolegend 424702). Alternatively ...
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bioRxiv - Microbiology 2024Quote: ... Zombie Aqua fixable viability kit was used to identify live cells (BioLegend). Cell numbers were calculated using counting beads (123 count eBeadsTM ...
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bioRxiv - Microbiology 2024Quote: ... Antibodies used were as follows: anti-c-kit-PE (clone 104D2, BioLegend), anti-CXCR4-Brilliant Violet 605 (clone 12G5 ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were permeabilized and blocked with 0.1% Triton X-100 in PBS supplemented with 10% normal goat serum for 1 hour at room temperature and incubated with mouse anti-Tuj1 (1:300; BioLegend) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Wells were cultured for 1 hour in a 10:1 ratio prior to being washed and stained with fluorochrome-conjugated Abs: anti-CD41 (clone MWReg- Biolegend) and anti-CD8 (clone 53.67- Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... Cells were labeled with live/dead dye (Zombie Aqua) for 10 min at RT and blocked with human TruStain FcX (BioLegend) for 20 min at RT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The suspended cells were then transferred to a fresh flask and activated in fresh RPMI-1640/10% FBS/PSG by 3:20,000 anti-CD3 (BioLegend 317303), 3:20,000 anti-CD28 (BioLegend 302913) ...
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bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Following isolations cells were centrifuged 10 min at 300 g and red blood cells were eliminated using Red blood cells (RBC) Lysis Buffer (BioLegend). This procedure gives >95% of neutrophil population as assessed by flow cytometry of CD15+/CD16+ cells ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1 × 106 cells per 100 μl in RPMI medium supplemented with 10%FBS and stimulated for overnight with Cell activation cocktail (Cat:423301, Biolegend) at 37°C incubator ...
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bioRxiv - Microbiology 2022Quote: ... the supernatant was discarded and the pellet was resuspended in 10 ml of cold 1X Red Blood Cell Lysis buffer (Biolegend). Samples were vortexed and incubated in the dark for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed twice with PBS plus 2 % FBS and subsequently coated with 10 μg/ml unconjugated anti-CD16 (3GB, BioLegend) in PBS overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Anti-Mac-1 was reconstituted by mixing anti-CD11b (clone ICRF44) and CD18 (clone TS1/18) mAbs at a final concentration of 10 µg/mL for each (BioLegend). Anti-CD3 mAbs (clone UCHT1 ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in 10% FCS in PBS containing Alexafluor647 anti-Ki67 (Biolegend; 1:200 in binding buffer ...
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bioRxiv - Immunology 2021Quote: IFNAR1 on WT and Mt3-/- BMDMϕ was neutralized using 10 μg/ml monoclonal anti-IFNAR1 antibody (BioLegend; Clone: MAR1-5A3) 1h prior and 24h after iLPS (10 μg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Immunology 2022Quote: ... or polarized to Th1 phenotype by culturing in T cell media additionally supplemented with IL-12 (20 ng/mL) and anti-IL-4 (10 µg/ml) (Biolegend). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2020Quote: In some cases we injected retro-orbitally with 10 μg of Alexa Fluor 647 anti-mouse CD31 (BioLegend, no. 110724) and 10 μg of Alexa Fluor 647 anti-mouse CD144 (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... tumor cell lines were plated in triplicate on 96-well plates and treated for 72 hours with 10 ng/mL TNF (Biolegend) or 0.1% BSA vehicle control ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Bioengineering 2020Quote: ... and then cells were fixed with 4% PFA at room temperature for 10 minutes and permeabilized with intracellular staining perm wash buffer (BioLegend), and stained with the appropriate intracellular antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Single tumor cells were resuspended to 5 × 107 cell/mL and stained with CD298-APC (10 µL/106 cells)(BioLegend) and Sytox Green (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Induced GC B-cells were then replated onto a fresh CD40LB feeder line and cultured with mouse IL-21 (10 ng/mL; BioLegend) or mouse IL-4 for another 3 to 4 days.
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 µL/well of either 10 µg/mL (flat-bottom) or 5 µg/mL (round-bottom) anti-mouse CD3ε (BioLegend) in PBS (for stimulated wells) ...
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... PBMC collected 17 weeks post-boost were incubated for 10 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Cell Biology 2022Quote: ... Co-cultures were fixed at 7-10 days in either 4% paraformaldehyde for 10 minutes or ice-cold 70% ethanol for 30 minutes prior to staining with anti-CD31-AF488-conjugated antibody (BioLegend) and anti-VWF (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... 5-10 x 106 cells were washed with PBS and stained with Zombie NIR Fixable live/dead dye (Biolegend, 423106) at 4 °C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were stained as above with efluor780 viability dye prior to incubation at 37C for 10 min with CCR7-APC-Fire810 (clone G043H7, Biolegend) and FcγR block ...