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Citations for BioLegend :
2101 - 2150 of 3473 citations for PLGF 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: Human T cells were concurrently stained with anti-CD4 Pacific Blue (OKT-4, Biolegend, USA), anti-CD8a APC (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... CD69 levels were measured with APC-conjugated anti-human CD69 antibodies (Biolegend, 310909, clone FN50). TCR levels were measured with BV421-conjugated anti-human TCR antibodies (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 550 µl of RPMI medium supplemented with 1 µg/ml recombinant human CCL21 (BioLegend; #582208) or nothing as negative control were added to the lower chambers ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was analyzed using the LEGENDPlex™ Assay (BioLegend; Human Inflammation Panel 1 (13-plex)) as per manufacturer’s protocols ...
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bioRxiv - Immunology 2023Quote: ... and isotype controls (mouse IgG1κ, clone MG1-45, BioLegend or human IgG1κ, clone QA16A12, BioLegend) were used as controls ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with anti-human IgG-APC (clone M1310G05, 1/250 dilution, BioLegend) in PBS-BSA for 20 minutes at 4°C ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: Surface marker staining was performed for 30min after 15min blocking with human TruStain FcX(BioLegend). Cells were washed in FACS buffer(PBS+2% FCS ...
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bioRxiv - Bioengineering 2023Quote: ... Human macrophages were stained with anti-CD11b (M1/70) and anti-CD64 (10.1) mAbs (Biolegend). Murine macrophages were stained with anti-CD11b (M1/70 ...
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bioRxiv - Bioengineering 2023Quote: ... The LEGENDplex™ Human Essential Immune Response Panel (13-plex) (BioLegend, San Diego, CA, USA) was employed to quantify the secreted cytokines in the cell culture supernatant following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 50µL ice-cold 1:10 human TruStain FcX (BioLegend 422302) in MACS buffer and incubated for at least 5 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... for 15 minutes and incubated with the human-specific anti-CD298-APC antibody (Biolegend, 341706) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was then stained with Alexa Fluor 488 anti-human CD3 antibody (BioLegend, 300320), PE anti-human CD25 antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... we included Ultra-LEAF™ purified anti-human TIGIT antibody (10μg/ml, clone A15153G, Biolegend) or its control isotype Ultra-LEAF™ purified mouse IgG2a antibody (10μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... The VEGF-A secretion amount was measured using a VEGF-A Human ELISA kit (Biolegend) by plate reader ...
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bioRxiv - Immunology 2022Quote: ... The TALL-104 cells were first stimulated with anti-human CD3 antibody (5μg/ml) (Biolegend) and anti-human CD28 antibody (5μg/ml ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with anti-CD1a (FITC anti-human CD1a Antibody, clone: HI149, BioLegend), anti-CD93 (PE conjugated anti-human CD93 Antibody ...
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bioRxiv - Immunology 2023Quote: ... 100 μL of cell suspension was mixed with Pacific Blue anti-human Ki-67 (Biolegend), followed by incubation at room temperature in the dark for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... the following fluorescence-conjugated antibodies were used in this study: Human TruStain FcX (BioLegend #422302), TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... for human samples and subsequently stained with live/dead cell exclusion dye (Zombie Dyes, BioLegend). Surface staining was performed with fluorophore-conjugated antibodies (Table S1 ...
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bioRxiv - Biochemistry 2023Quote: ... in the presence of Human TruStain FcX™ Fc Receptor Blocking Solution (1:400, BioLegend), and fixed with 0.5 mL methanol-free paraformaldehyde 4% (Thermo Scientific™ Pierce™ ...
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bioRxiv - Immunology 2024Quote: ... The ultrasound-guided intra-amniotic injection of recombinant human HMGB1 (Biolegend, San Diego, CA, USA) at a concentration of 9 ng dissolved in 100 μL of sterile 1X phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2024Quote: ... PE/DazzleTM 594- or FITC-conjugated anti-human CD45 (clone 14C2A07, BioLegend; clone SA231A2, BioLegend), PE/Cy7 anti-human CD16 (clone 3G8 ...
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bioRxiv - Immunology 2023Quote: ... 24-well plates have been coated with 1μg purified human TCRγ/δ antibody (331202, BioLegend) per well overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... TCR levels were measured with BV421-conjugated anti-human TCR antibodies (Biolegend, 306722, clone IP26) or anti-mouse TCR antibodies (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... PE/DazzleTM 594- or FITC-conjugated anti-human CD45 (clone 14C2A07, BioLegend; clone SA231A2, BioLegend), PE/Cy7 anti-human CD16 (clone 3G8 ...
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bioRxiv - Immunology 2024Quote: ... Donor 1 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... FITC-conjugated anti-mouse/human CD11b and APC-conjugated anti-mouse CD206 antibodies (BioLegend, USA). After surface staining ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by surface antigen staining with PE-labelled anti-human CD82 (Biolegend, clone TS2/16) and Alexa 488-labelled anti-human CD44 (eBioscience ...
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bioRxiv - Cell Biology 2024Quote: ... human donor RGCs were pretreated with 5 mg/mL annexin V (BioLegend, San Diego, CA), a calcium-dependent membrane-bound protein with high affinity to PS ...
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bioRxiv - Bioengineering 2024Quote: ... The cytokine profile was assessed using the LEGENDplex™ Human M1/M2 Macrophage Panel (Biolegend) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and pre-treated with a mixture of human TruStain FcX (Fc Receptor blocking solution, Biolegend) and CD16/CD32 Rat anti-mouse Fc antibodies (mouse BD Fc blocker ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used to stain the cells: BV711-conjugated anti-human CD4 (BioLegend); APC-conjugated anti-human PD1 (BioLegend) ...
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bioRxiv - Genomics 2024Quote: ... Fc receptor blocking was performed using a 1:50 dilution of Human TruStain FcX (Biolegend) in cell staining buffer for 10 minutes at 4 degrees ...
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bioRxiv - Cancer Biology 2024Quote: ... Conjugated antibodies used for cell labeling were as follows: APC anti-human CD80 (Biolegend 375403), PE anti-human CD163 (Biolegend 333605) ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 711™ anti-mouse/human CD11b antibody (1:200; Biolegend, Clone M1/70), and FITC anti-mouse/human CD45R/B220 antibody (1:200 ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... the following primary antibodies were used: Phycoerythrin (PE)-conjugated anti-human TMPRSS2 antibody (BioLegend #378403) and CR3022 (generated as described below) ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Bioengineering 2021Quote: ... Bcl-2 anti-apoptotic protein expression was measured by intracellular staining of BMMs with PE-Cy7 anti-Bcl-2 antibody (Biolegend). AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2020Quote: ... single-cell IEL suspension was incubated with staining buffer (PBS containing 2% FBS with 2 mM EDTA) containing a mixture of biotin-conjugated mAbs against CD4 (RM4-5; BioLegend), CD19 (6D5 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Immunology 2023Quote: ... we coated 96 well plates with indicated amounts of anti-CD3ε antibody supplemented with soluble anti-CD28 at 2-times the amount of anti-CD3ε and 5U/mL hL IL-2 (Biolegend). For activation ...
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bioRxiv - Cancer Biology 2023Quote: ... Hepatocytes were resuspended in 2 mL FACS buffer (2 mM EDTA, 0.5% BSA in PBS) and stained with Zombie Violet (1:500) (Biolegend #423113), TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2023Quote: ... biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) from BioLegend. SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed 3 times for 5 minutes each with 1xPBS + 0.03% Triton before incubating for 2 hours in secondary solution containing 2% NDS + DAPI (1:1000; Biolegend 422801) + secondary antibodies (Alexafluor 647 donkey anti-rabbit ...
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bioRxiv - Immunology 2024Quote: ... splenocytes (2*107/mL in IMDM medium containing 2% FCS) were simultaneously stained with anti-B220-Alexa647 (clone RA3-6B2 Biolegend) and loaded with 5 μM Indo-1 acetoxymethyl (Indo1-AM ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse CD63-160Gd (clone NVG-2, BioLegend), anti-mouse CD120b-166Er (clone TR75-32.4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse IL-2 (clone JES6-1A12, BioLegend) antibody was used to capture IL-2 from the supernatants ...