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Citations for BioLegend :
2101 - 2150 of 3466 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Cancer Biology 2019Quote: ... Red blood cell lysis was performed and resulting leukocytes were resuspended in PBS + 1% BSA and blocked for 10 mins on ice with FC block and stained with Sytox® blue viability marker and conjugated antibodies anti-CD45-APC/Cy7 (Biolegend, 103115), anti-CD11b-APC (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... in FACS buffer for 30 minutes at 4°C followed by 2 wash steps with FC stain buffer and resuspended in Fluorofix (BioLegend, cat. 422101) for 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Fixable Viability Stain 510 for 15 minutes at 4 °C followed by anti-Fc blocking reagent (Biolegend, Cat# 101320) for 10 minutes prior to surface staining ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted down at 1,500 rpm at 4°C for 5 min and cell pellets were resuspended in 100 uL FACS buffer (PBS + 4% FBS +0.5 mM EDTA) containing Fc block (BioLegend, 101302, 1:200). After 15 min incubation at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Biophysics 2021Quote: ... Synomag-D+ DC were intradermally injected into each hind footpad immediately following Tag-It Violet™ (Violet+) cell tracking dye incorporation (Biolegend, San Diego, USA). MPI was done immediately following injection (day 0 ...
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bioRxiv - Immunology 2019Quote: ... After isolation cells were treated with Fc receptor blocking solution and stained with anti-ICAM-1-PE (1 μg/ml, HA58; Biolegend, San Diego, USA), anti-VCAM-1-BV711 (0.5 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... FCS files were evaluated with the LEGENDplex™ Data Analysis Software Suite using the Qognit cloud base platform (Biolegend, San Diego, CA, USA). A five-parameter logistic curve-fitting method was used to calculate concentrations from Median Fluorescence Intensity and normalized to the amount of protein in each sample ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... cells were washed with 200 μl FACS Buffer and blocked in 100 μl Fc block (cat. no. 101319, TruStain FcX, clone 93, BioLegend, diluted 1:100) in FACS Buffer for 10 min on ice and washed ...
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bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots were thawed and incubated with the panel of antibodies following the blockade of non-specific binding of the immunoglobulin to the Fc receptors by TruStain FcX (BioLegend, San Diego, CA). LIVE/DEAD Fixable Near-IR Dead Cell Stain dye (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Immunology 2023Quote: ... tumor cell suspensions were incubated 10 min at RT with Fc block (Leico; C381) and a viability dye (Biolegend; Zombie Aqua (ref # 423102) or Zombie NIR (ref # 423106) ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained using the anti-human monoclonal antibodies fluorophore-conjugated (Biolegend) Brilliant Violet 605™ anti-human CD45 (HI30) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-human CD326 PE (BioLegend; Cat#324206; used at 1:200), and mouse IgM anti-human HTII-280 (Terrace Biotech ...
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bioRxiv - Immunology 2021Quote: ... 100 μl anti-human IgG or IgM - horseradish peroxidase (HRP) conjugates (BioLegend), diluted 1:2500 and 1:5000 in dilution buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD309 (KDR/VEG-FR2) (Biolegend, clone 7D4-6; 1:200); anti-human CD184 (CXCR4 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human FcER1 BV510 (1:100, clone AER-37, BioLegend, cat # 334626), Anti-human CD303 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD163 PE (1:50, clone GHI/61, BioLegend, cat # 333606), Anti-human CD49d PE Dazzle 594 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD56 APC Cy7 (1:50, clone HCD56, BioLegend, cat # 318332), Anti-human CD45 APC Cy7 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human PD-1 BV785 (1:50, clone EH12.2H7, BioLegend, cat # 329930), Anti-human CD45 BUV395 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD4 BV510 (1:100, clone RPA-T4, BioLegend, cat # 300546), Anti-human CD56 BV605 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human MerTK PE Cy7 (1:50, clone 590H11G1E3, BioLegend, cat # 367610), Anti-human TIGIT PE Cy 7 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD103 BV711 (1:100, clone Ber-ACT8, BioLegend, cat # 350222), Anti-human CD10 BV711 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human Granzyme K PE (1:25, clone GM26E7, BioLegend, cat # 370512), Anti-human FOXP3 PE CF594 (1:25 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CXCR6 PerCP Cy5.5 (1:50, clone K041E5, BioLegend, cat # 356010), Anti-human CCR8 PE (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CXCR4 APC Cy7 (1:50, clone 12G5, BioLegend, cat # 306528), Anti-human CTLA4 APC Cy7 (1:25 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD8a BV650 (1:50, clone RPA-T8, BioLegend, cat # 301042), Anti-human CD326 BV650 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CTLA4 APC Cy7 (1:25, clone BNI3, BioLegend, cat # 369634), Anti-human CD56 APC Cy7 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human HLA-DR BV785 (1:50, clone L243, BioLegend, cat # 307642), Anti-human PD-1 BV785 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45 APC Cy7 (1:100, clone HI30, BioLegend, cat # 304014), Anti-human CD14 APC eF780 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD8a PerCP (1:50, clone RPA-T8, BioLegend, cat # 301030), Anti-human CXCR6 PerCP Cy5.5 (1:50 ...
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bioRxiv - Immunology 2019Quote: ... The cells were washed and resuspended with Human TruStain FcX (Biolegend; 422302) for five minutes before adding the primary surface staining cocktail for 20m ...
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bioRxiv - Immunology 2020Quote: ... Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody (Clone HCD56, Biolegend), anti-CAR antibody Goat F(ab’)2 anti-Human IgG (H+L)-Alexa Fluor 647 (Jackson) ...
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bioRxiv - Immunology 2019Quote: ... cells were pre-incubated with Human TruStain FcX™ (BioLegend, catalog 422301) to block Fc receptors ...