Labshake search
Citations for BioLegend :
2051 - 2100 of 6274 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for >5 minutes at 4C with Fc Block (clone 93; BioLegend), then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies ...
-
bioRxiv - Developmental Biology 2022Quote: ... for 5-minutes on ice and stained with PECAM1-BV605 (Biolegend, #102427, 1:1000, 100), CD45-FITC (Biolegend ...
-
bioRxiv - Cancer Biology 2019Quote: ... YAP1 was co-stained with cytokeratin 5 (CK5) or 14 (CK14) (BioLegend, San Diego, CA) and imaged with a Nikon fluorescence microscope (Melville ...
-
bioRxiv - Immunology 2021Quote: ... 2×106 cells were incubated for 5 hours with Brefeldin A (BioLegend, San Diego, California) and Monensin (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
-
bioRxiv - Immunology 2020Quote: ... Fc receptors were first blocked for 5 minutes using CD16/CD32 (Biolegend, San Diego, CA). Eosinophils were identified as CD11c-Siglec-F+ and neutrophils were identified as SiglecF-GR-1+ ...
-
bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
-
bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were stained with 2.5ug of anti-CD4-APC (RM4-5, Biolegend 100516) antibody in 1mL of sort buffer and incubated at 4°C for 30 minutes ...
-
bioRxiv - Immunology 2022Quote: ... 5×105 SABR expressing NFAT-GFP-Jurkat cells were labeled with cell trace violet (BioLegend) according to the manufacturer’s protocol before incubation with 5×105 TCR expressing Jurkat cells in a round bottom 96-well plate for 16-20 hr ...
-
bioRxiv - Immunology 2022Quote: ... Omicron RBD (BA.2 or BA.5) were labeled with TotalSeq-C0971 Streptavidin (Biolegend, 405271) and TotalSeq-C0972 Streptavidin (Biolegend ...
-
bioRxiv - Physiology 2022Quote: ... and then stained for subsets of markers at 5 μg/ml: Ly6G-BV421(Biolegend, 127628), Ly6C-BV605 (Biolegend ...
-
bioRxiv - Neuroscience 2024Quote: ... spun for 5 minutes at 300 × g and washed once with 1x staining buffer (BioLegend). iNSCs were stained in suspension for 30 minutes at 37°C on an orbital shaker (500 rpm) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Then resuspend and count in 1640 complete medium containing CD28 (5 μg/ml, 302934, Biolegend) and IL-2 (10 ng/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... and matched isotype controls APC mouse IgG2b k isotype control (5 μL/test; BioLegend #400322), APC Rat IgG2b ...
-
bioRxiv - Immunology 2023Quote: ... and cytokines (IFN-y and IL-5) were measured by ELISA (Biolegend. San Diego, CA). For intracellular cytokine determination ...
-
bioRxiv - Immunology 2023Quote: ... Samples were then stained overnight at 4C with anti-CD4 (AlexaFluor488, clone RM4-5, BioLegend) and anti-CD45.1 (Alexafluor647 ...
-
bioRxiv - Immunology 2023Quote: ... Cells were stained with anti-CD64 (139304, PE anti-mouse CD64, clone:X54-5/7.1, BioLegend) and anti-CD11b (25-0112-81 ...
-
bioRxiv - Immunology 2023Quote: ... we injected 5 μg of PE-Cy7 dye-conjugated anti-CD45 (Clone 30-F11, BioLegend) monoclonal antibodies (mAb ...
-
bioRxiv - Immunology 2023Quote: ... macrophages were challenged with CFSE (5-[and 6]-Carboxyfluorescein diacetate succinimidyl ester Kit, BioLegend,423801)-labeled apoptotic cells at a 4:1 (AC/Macrophage ...
-
bioRxiv - Cell Biology 2024Quote: ... human donor RGCs were pretreated with 5 mg/mL annexin V (BioLegend, San Diego, CA), a calcium-dependent membrane-bound protein with high affinity to PS ...
-
bioRxiv - Immunology 2022Quote: ... LEAF Purified anti-mouse CD3ε Antibody (100313) and LEAF Purified anti-mouse CD28 Antibody (102111) were purchased from Biolegend (San Diego, CA, USA). Staphylococcus Entrotoxin B (BT202 ...
-
bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-CD201 antibodies conjugated to PE and anti-CD48 antibodies conjugated to APC or PE-Cy5 were purchased from Biolegend (San Diego, CA). To stain dead cells ...
-
bioRxiv - Immunology 2022Quote: ... Fc receptors were blocked with anti-mouse CD16/CD32 antibodies and stained with fluorophore or biotin-conjugated anti-mouse antibodies (BD Pharmingen, Biolegend, Tonbo or eBioscience) to detect B220 (RA3-6B2) ...
-
bioRxiv - Cell Biology 2021Quote: ... cryosections were probed with antibodies against Thy1 (Alexa Fluor 488 anti-mouse CD90.2 Antibody, Biolegend 105315, or Alexa Fluor 647 anti-mouse CD90.2 Antibody, Biolegend 105318, both Clone, 30-H12), Clu (Polyclonal Rabbit antiHuman CLU / Clusterin ...
-
bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were washed and stained with fluorochrome-conjugated CD56 antibody for NK-92 cells and CD11b antibody for primary neutrophils (Biolegend, San Diego, CA) for 30□min on ice ...
-
Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...
-
bioRxiv - Biophysics 2023Quote: ... we incubate 5-6*105 NK92 cells prior to mixing them into the collagen for one hour in serum-free cell culture medium at 37°C with or without the following antibodies: 10 μg/ml anti-integrin activating β1 (CD29) antibody (Biolegend, cat. no. 303002) and the corresponding IgG1 isotype (Biolegend ...
-
bioRxiv - Cell Biology 2024Quote: ... β-III-tubulin antibodies TUJ1 and TUBB3 (801202 and 802001) as well as neurofilament monoclonal antibody SMI312 (837904) were from BioLegend (San Diego, CA). GFP antibody (12A6 ...
-
bioRxiv - Biophysics 2021Quote: Flow channels were constructed as previously described but anti-TAMRA antibody was replaced with Anti-ß3 tubulin antibody (BioLegend Poly18020 anti-Tubulin Beta 3). Microtubule nucleation was achieved by introducing 5-50 μM tubulin (1:17 molar ratio of Atto-633 labelled:unlabelled ...
-
bioRxiv - Bioengineering 2021Quote: ... IL-6 was quantified using ELISA developed in the laboratory using biotin anti-rat IL-6 antibody (cat. # 517703) and purified (coating) anti-rat IL-6 antibody (cat. # 517701, Biolegend, San Diego, CA, USA). Coating antibody was diluted in 100 mM NaHCO3 ...
-
bioRxiv - Bioengineering 2020Quote: PDCD1 and B2M expression on EL4 cells were assessed with fluorescent antibody staining using an APC-conjugated PD1 antibody (BioLegend, San Diego, CA: 135210) or AlexaFluor 647-conjugated H-2Kb antibody (BD Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... OVA peptide-conjugated MHC-I complex and MHC-II were labeled with anti-H2-kb- SIINFEKL antibody and anti-MHC II antibody (BioLegend, San Diego, CA, USA), respectively to assess antigen presentation and CFSE fluorescence was measured to assess phagocytosis ...
-
bioRxiv - Immunology 2022Quote: ... 5 x 105 cells from each sample were stained for combinatorial hashing using oligonucleotide-conjugated Hashtag antibodies targeting the ubiquitous cell surface antigens Beta-2-Microglobulin and CD298 (Biolegend Totalseq A Hashtag antibodies). Hashed samples were pooled and stained together with a cocktail of 73 oligonucleotide-conjugated antibodies targeting cell surface antigens ...
-
bioRxiv - Neuroscience 2024Quote: ... A third group was injected with CD16/32 neutralizing monoclonal antibodies (Ultra-LEAF Purified Anti-mouse CD16/32 Antibody, Biolegend, San Diego, CA, USA) in a volume of 200 µL/mouse (10 mL/kg ...
-
bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...
-
bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
-
bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
-
bioRxiv - Cell Biology 2020Quote: ... treated cells were harvested and stained with 5 μg/ml propidium iodide and Annexin V (Biolegend) in Annexin V-binding buffer (10 mM Hepes pH 7.4 ...
-
bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
-
bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
-
bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
-
bioRxiv - Systems Biology 2021Quote: ... centrifuged and resuspended in 5 ml of 1X red blood cell (RBC) lysis buffer (420301, BioLegend) for 5 min on ice ...
-
bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
-
bioRxiv - Cell Biology 2022Quote: ... BV711 Mouse anti mouse CD64 (Monoclonal; clone X54-5/7.1; Biolegend; 1:100; Cat#139311, FACS). BV421 Rat anti mouse CD68 (Monoclonal ...
-
bioRxiv - Genomics 2022Quote: ... and extracellular stains with anti-mouse CD4-APC (1:400, clone RM4-5, BioLegend Cat#100516), anti-mouse TCRb chain-FITC (1:400 ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were stained for 15 min in the dark with 5 μL APC Annexin V (Biolegend) and 5 μL 7-AAD Viability Staining Solution (Biolegend) ...
-
bioRxiv - Immunology 2022Quote: ... the cell suspension was incubated in 5 μgml−1 anti-CD16/32 (#101302, Biolegend, 1:200), stained with Live Dead Near IR (#L10119,ThermoFisher ...
-
bioRxiv - Immunology 2023Quote: ... 5 × 105 NPCs were labeled with viability dye marker (Zombie Aqua, Biolegend, San Diego, CA, USA) and blocked with PBS supplemented with 1% Fc Block (MACS ...