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Citations for BioLegend :
2051 - 2100 of 2728 citations for Recombinant Human GLB1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Neuroscience 2020Quote: ... antibody immunogenized against human ACE2 and a mouse Tuj1 antibody against neuronspecific tubulin (BioLegend, 801201; RRID:AB_2313773). Anti-ACE2 was raised against a C-terminal synthetic peptide for human ACE2 and was validated by the manufacturer to not cross-react with ACE1 for immunohistochemical labeling of ACE2 in fruit bat nasal tissue as well as in human lower airway ...
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bioRxiv - Cell Biology 2019Quote: THP-1 cells were labeled with mouse antibody anti-human CD18 (PE BioLegend or BV421 BD) (1 µl per 200 000 cells ...
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bioRxiv - Bioengineering 2021Quote: Cells were stained with the following antibodies: PE anti-human CD4 (clone RPA-T4, Biolegend 300507), FITC anti-human CD8a (clone RPA-T8 ...
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bioRxiv - Biochemistry 2020Quote: ... half the cells were stained for surface CD4 (anti-human CD4, Biolegend, conjugated directly to APC), fixed in formaldehyde ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Immunology 2021Quote: Cytokines were measured in plasma samples using the LEGENDplex™ human inflammation assay (cat. # 740809, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and as previously described using the anti-human CD34 antibody11 (Clone 581; Biolegend, San Diego, CA).
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bioRxiv - Cancer Biology 2021Quote: ... Samples were immediately stained with 5μL of either PE anti-human CD261 (DR4) (Biolegend, Clone DJR1), PE antihuman CD262 (DR5 ...
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bioRxiv - Cancer Biology 2022Quote: ... For blood-derived monocytes phenotyping, FITC-anti-human CD14 (Miltenyi, #130-110-576) antibody from Biolegend was also used.
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bioRxiv - Immunology 2022Quote: ... The following antibodies were used for surface staining: PerCP/Cy5.5 anti-human CD4 (BioLegend, cat. 317428), PacificBlue anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... including mouse α-αM (clone ICRF44 for human experiments, clone M1/70 for mouse experiments, Biolegend), mouse anti-human/mouse α3 (α-α3 ...
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bioRxiv - Immunology 2022Quote: ... Whole blood cell staining was performed using an Fc receptor blocking solution (Human TruStain FcX; BioLegend), the viability dye eFluor780 (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with monoclonal mouse anti-human Lamp-1-AlexaFluor488 (BioLegend 328610, clone H4A3) at a final dilution of 1:500 for 1 hours ...
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bioRxiv - Immunology 2020Quote: ... Cells were pelleted again and resuspended in 100 ul of Fc-Block (Human TruStain FcX, Biolegend), and 10 ul aliquot was taken for counting using K2 Cellometer (Nexcelom ...
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bioRxiv - Immunology 2020Quote: ... Proteins were detected by immunoblotting using mouse anti-human CD9 (clone HI9a, BioLegend, dilution 1:1.000), mouse anti-human CD63 (clone TS63 ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Immunology 2022Quote: ... followed by rat anti-human Fc antibody conjugated to APC (Cat: 410712, BioLegend; 1:100 dilution) for 15 min on ice in the dark for secondary detection ...
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bioRxiv - Immunology 2022Quote: ... or 50ng/ml anti-human ICOS-L or 50ng/ml of the corresponding isotype control (Biolegend). After 60 min ...
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bioRxiv - Immunology 2022Quote: ... and a human cell surface protein antibody panel containing 277 oligo-conjugated antibodies (TotalSeq-A, BioLegend) including 6 TotalSeq-A isotype controls (table S4) ...
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bioRxiv - Immunology 2022Quote: ... anti-CD66b antibody (pacific-blue conjugated mouse anti-human, clone G10F5, BioLegend, San Diego, CA, USA) and anti-CD15 antibody (phycoerithrin-cyanine 7 conjugated mouse anti-human ...
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bioRxiv - Immunology 2020Quote: Human PBMCs were isolated by Ficoll and incubated with antibodies against CD3 (OKT3, BioLegend unless indicated), CD4 (RPA-T4) ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNF-α using the LEGENDplex™ human inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as per manufacturer’s instructions ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... Ultra-LEAF™ purified anti-human CD95 (FAS) blocking antibody (10 μg/ml, clone A16086F, Biolegend) or respective DMSO and H2O control were added prior to incubation ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Immunology 2019Quote: ... at 0.1μg/ml each in 100μl total volume: AF647-conjugated anti-human/mouse CD11b (#101220, BioLegend), PE-conjugated anti-mouse Ly6C (#101220 ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea pig anti-Cytokeratin 8+18 antibody and FITC rat anti-mouse/human CD45R/B220 (BioLegend) were used for fluorescence immuno-histochemistry ...
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bioRxiv - Synthetic Biology 2021Quote: ... and the cell lysates were assessed on a human IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Genomics 2021Quote: ... 4 µl of the respective antibodies were used: APC anti-human SSEA-4 antibody (Biolegend, #330417) and PE anti-human TRA-1-60-R antibody (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with Alexa Fluor® 647-conjugated anti-human IFN-γ antibody (502516, Biolegend) and PE-conjugated anti-human IL-17A antibody (512305 ...
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bioRxiv - Immunology 2021Quote: ... Cytokine levels were analyzed by LegendPlex TGF-β 1 and multiplex human inflammation panel assay (Biolegend). All nine parameters were detectable in every sputum sample derived from patients with AR and AA and compared to healthy control subjects.
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bioRxiv - Immunology 2020Quote: ... BMDM were labeled with APC anti-mouse/human CD11b (1:400, Cat. 101211, BioLegend CA, USA) and PE anti-mouse CD45 (1:400 ...
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bioRxiv - Immunology 2021Quote: ... eBioscience Cat#12-1239-42) and CD304 (BV421 anti-human CD304, clone 12C2, BioLegend Cat#354514) (Appendix Figure S1) ...
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bioRxiv - Immunology 2022Quote: ... Jurkat cells were labelled with 25 μg/ml anti-human CD3 antibody (BioLegend, 317304, clone OKT3) and 3 μg/ml recombinant annexin V (BioLegend ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... using ELISA MAX Deluxe Set human (IFNγ #430104, IL-2 # 431804, and IL-6 #430504, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... MHC-II (LGII-612.14, CST, Catalog: 68258S) and Anti-human pan-Cytokeratin-Alexa647 (Biolegend, Catalog: 628604) were used for the staining ...
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bioRxiv - Genomics 2022Quote: ... resuspended in chilled 1% BSA in PBS and incubated with human TruStain FcX blocking solution (BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cytokine secretion in the supernatant was evaluated by ELISA MAX Deluxe Set Human TNF-α (BioLegend), ELISA MAX Deluxe Set Human IFN-γ (BioLegend) ...
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bioRxiv - Genetics 2022Quote: ... for SynT and HLAG (1:100, APC anti-human HLA-G Antibody Clone: 87G, BioLegend UK) for EVT ...
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bioRxiv - Microbiology 2022Quote: ... blocked with Fc Block for 20 min at room temperature (Human TruStain FcX, Biolegend Cat# 422302), and simultaneously stained for surface antigenic Site Ø ...
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bioRxiv - Immunology 2022Quote: ... and stained again with biotinylated anti-mouse/human TLR7/8 mAb and PE streptavidin (Biolegend, USA). To detect intracellular mouse IL-6 in splenocytes stimulated with various ligands in the presence of Brefeldin A (10μg/ml) ...
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bioRxiv - Immunology 2023Quote: ... These slides were incubated overnight with anti-human CD34 FITC (Clone 581, Biolegend 343504, 1:200), anti-human/mouse ACTA2 Cy3 (Clone 1A4 ...
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bioRxiv - Systems Biology 2022Quote: ... and resuspended in 25μL cell staining buffer and 25ul of TotalSeqA Human Universal Cocktail v1.0 (BioLegend) for 30min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... HPCs were validated by staining them with 1:100 dilution of CD34 (anti-human FITC, Biolegend), CD43 (anti-human PE ...
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bioRxiv - Neuroscience 2022Quote: ... 4B12 [a mouse monoclonal antibody that recognises residues 103-108 of human α-synuclein (12)] (BioLegend) at 1:250 and MJFR14 [a rabbit monoclonal antibody that has been reported to recognise assembled human α- synuclein (15)] (Abcam ...
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bioRxiv - Immunology 2023Quote: ... the supernatant from cocultures was collected and measured by LEGENDplex™ Human CD8/NK Panel (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: The antibodies used for flow cytometry are Alexa Fluor 647 mouse anti-human BST2 (Biolegend; 348404) and HIV-1 core antigen-FITC (KC57 ...