Labshake search
Citations for BioLegend :
2051 - 2100 of 2285 citations for Rat Interleukin 8 Receptor Beta IL8RB CXCR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
-
Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... CD8 OT-I cells and CD4 OT-II were isolated by MojoSort kit purchased from BioLegend (#480035, #480033) according to the manufacturer protocol ...
-
bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
-
bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
-
bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... cells were washed with PBS and stained for dead cells using Zombie UV Fixable Viability Kit (Biolegend, 423108) or Zombie NIR Fixable Viability Kit (Biolegend ...
-
Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: CD8+ naïve T cells were isolated from spleen using Mojosort Mouse CD8 Naïve T cell isolation Kit (Biolegend) and adjusted to a concentration of 1.0×106 cells/mL ...
-
bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to staining using the Annexin V/Propidium Iodide (PI) apoptosis assay kit (BioLegend, USA) and subsequently analyzed using a BD FACSCalibur flow cytometer (USA) ...
-
bioRxiv - Cell Biology 2021Quote: Detection of apoptotic cells was done by using FITC-Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend#640922). Briefly ...
-
bioRxiv - Systems Biology 2019Quote: ... and IL-6 secreted protein level in cell supernatant from 1×106 human MoDM’s was performed by Legend Plex multi-analyte assay kit (BioLegend) according to manufacturer’s instruction ...
-
bioRxiv - Genomics 2021Quote: ... for RNA library construction while modifications for CITE-seq ADT and cell hashing HTO library preparation were performed according to the TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 single index protocol (BioLegend). The modifications include the addition of ADT and HTO additive primers to a final concentration of 0.2 uM in the cDNA amplification reaction (step 2.2) ...
-
bioRxiv - Immunology 2021Quote: ... The cells were washed with 1xPBS and fixed using buffer of the True-Nuclear Transcription Factor Staining kit (Biolegend). After fixing ...
-
bioRxiv - Cell Biology 2021Quote: ... CD4+ T cells were then isolated by negative selection using the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend). Isolated CD4+ T cells were seeded at 1×106/mL in RPMI supplemented with 10% FBS ...
-
bioRxiv - Immunology 2022Quote: ... Brefeldin A solution (420601) and CFSE Cell Division Tracker Kit (423801) were purchased from Biolegend (San Diego, CA, USA). PE anti-mouse IL-4 (12-7041-81) ...
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
-
bioRxiv - Immunology 2022Quote: ... The percentage of apoptotic cells were then determined using the FITC Annexin V apoptosis detection kit with PI (BioLegend) and flow cytometry as per manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from naïve gender- and age-matched WT and CD6Δd3 splenocytes by negative selection using magnetic beads (Mojo sort kit, BioLegend). Isolated cells were activated with 3 μg plate-bound anti-mouse CD3 (145-2C11 ...
-
bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
-
bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
-
bioRxiv - Physiology 2019Quote: CD4+CD25+ cells were isolated using negative selection of CD4+ T cells using the MojoSort Mouse CD4 Naïve T-cell Isolation Kit (catalog #480040, Biolegend) followed by positive selection of CD25+ ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were prepared for flow cytometry two days after media change using the FITC Annexin V kit (Biolegend, 640906). Quantification was performed using a BD FACSCanto 10 flow cytometer.
-
bioRxiv - Neuroscience 2020Quote: ... cells were harvested and stained with AV and PI according to manufacturer’s manual (FITC Annexin V Apoptosis Detection Kit with PI; Biolegend). Stained cells were immediately analyzed by flow cytometry (MACSQuant ...
-
bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
-
bioRxiv - Bioengineering 2021Quote: ... and the supernatant was used to measure secreted cytokines using a LEGENDplex™ Mouse Th Cytokine Panel kit (BioLegend) according to the manufacturer’s instruction ...
-
bioRxiv - Cancer Biology 2021Quote: ... Apoptosis was determined by detecting phosphatidylserine using the APC-Annexin-V Apoptosis Detection Kit with PI (Biolegend, CA, USA). Briefly cells were harvested ...
-
bioRxiv - Cell Biology 2020Quote: ... total T cells or naïve CD4+ T cells were isolated from mouse spleen and lymph nodes using MojoSort mouse CD3/CD4 naïve T cell Isolation Kit (Biolegend) following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
-
bioRxiv - Immunology 2020Quote: CD4+ T cells were harvested from the lymph nodes and spleens of naive NR1 mice and enriched with a mouse naïve CD4 negative isolation kit (BioLegend) following the manufacturer’s protocol ...
-
bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized with a Transcription Factor Staining Kit (eBioscience) and stained using anti-IFNγ (XMG1.2; 200x; BioLegend) and recombinant mouse IL21R Fc Chimera (600x ...
-
bioRxiv - Immunology 2020Quote: CD8 T cells were isolated from an OT1+ mouse using the mojosort mouse CD8 T cell isolation kit (Biolegend) and labeled with violet proliferation dye (BD Biosciences cat# 562158) ...
-
bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
-
bioRxiv - Cancer Biology 2020Quote: ... T cells were isolated from spleen using MojoSort™ Mouse CD3 T Cell Isolation Kit (Biolegend Cat no; 480024) as per the manufacturers protocol ...
-
bioRxiv - Immunology 2021Quote: For differentiation of live and dead cells we stained cells with the Zombie Violet Fixable Viability Kit according to the manufacturer’s instructions (BioLegend). For surface marker analysis ...
-
bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
-
bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated from the spleen by using the MojoSort Mouse Naïve CD4+ T Cell Isolation Kit (#480040, BioLegend). CD4+ T cells isolated from the OT-II spleen were cocultured with BMDCs ...
-
bioRxiv - Immunology 2022Quote: ... cells were washed again using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Biolegend; 423101) for 20 min at room temperature ...
-
bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
-
bioRxiv - Microbiology 2022Quote: ... Apoptotic cells were detected using the PE Annexin V Apoptosis Detection Kit with 7-AAD according to the manufacturer’s protocol (BioLegend) and analyzed using an LSRII flow cytometer.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and labeled with propidium iodide (PI) and Annexin-V-FITC (Annexin V FITC Apoptosis Detection Kit with PI, Biolegend) for the detection of necrosis and apoptosis ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
-
bioRxiv - Cell Biology 2024Quote: ... untouched primary naïve T cells were isolated with an EasySep Mouse T cell Isolation Kit according to the manufacturer’s protocol (BioLegend). The purified CD4+ Naïve T cells were stimulated with 20 ng/mL phorbol 12-myristate13-acetate (PMA ...
-
bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
-
bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells isolated from the mouse spleen using the MojoSort Mouse Naïve CD4 T Cell Isolation Kit (#480040, BioLegend) were stimulated in a plate coated with LEAF purified anti-mouse CD3ε antibody (clone 145-2111C ...
-
bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
-
bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
-
bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
-
bioRxiv - Immunology 2023Quote: ... Supernatant was harvested and cytokines were quantified using a Legendplex Mix and Match Kit according to manufacturer instructions (Biolegend).