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Citations for BioLegend :
2051 - 2100 of 2702 citations for 7 Chloro 5 methyl 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804; GluGlu: Biolegend #901801 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated for 30 minutes at 4°C in PBS with a fluorochrome-conjugated antibody (mouse monoclonal APC-conjugated anti-CD69 (BioLegend 10909); mouse monoclonal PE-conjugated anti-CD25 (Pharmingen 30795X ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocking of Fc receptors was performed by incubation at 4°C for 20 minutes with an unlabelled anti-CD16/32 (1 μg/million cells; BioLegend – 101320). The cells were then incubated for one hour at 4°C in the dark with a mix of rat anti-mouse CD41 (labelled with Phycoerythrin ...
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bioRxiv - Pathology 2022Quote: ... Cells were washed (PBS; twice), centrifuged (300g; 5min.; 4°C) and stained (10min., RT) with Streptavidin (Alexa Fluor® 647-conjugated, Biolegend, #405237 ...
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bioRxiv - Cancer Biology 2019Quote: ... and stained with the following antibodies in isolation or in combination for 30-60 minutes in the dark at 4°C: ICAM-1 PE (BioLegend, #353106), CCR6 PE/Dazzle (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... for 10 minutes at 4°C and then incubated for 30 min at room temperature with primary antibodies including CD45-BV785 (BioLegend, 103149) CD11b-PE (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with the following primary mouse antibodies (1:100 dilution) for 30 minutes at 4°C: CD45-BV750 (clone 30-F11, Biolegend, 103157), CD31-PE-Cy5.5 (clone MEC 13.3 ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Immunology 2021Quote: Four different TotalSeq™-C antibodies were used for Hash Tag Oligonucleotide (HTO) labelling before cell sorting (Biolegend, M1/42M30-F11) following the manufacturer’s standard protocol ...
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bioRxiv - Immunology 2020Quote: ... was incubated with 1-2 spike+ cells for 1 hour at 4°C before adding of 0.02 ug/ml ACE2-mFc followed by FITC conjugated anti-mouse IgG (Biolegend, cat#405305) and the MFI of FITC and positive percentage were collected.
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bioRxiv - Immunology 2020Quote: ... cells were washed with PBS twice before staining with the following antibodies or reagents for another 30 min at 4°C in the dark: Streptavidin-BV650 (Biolegend, UK), IgG (H+L ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Immunology 2022Quote: ... Immunostaining was performed by incubating the sections overnight at 4°C with the SMI-32 primary antibody (Biolegend: Suppl. Table 1) in incubation buffer ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 10 min at 4°C followed by an incubation with CD45-BV421 (Biolegend 103133, clone 30-F11, 1:200 dilution) and Ter119-BV421 (Biolegend 116233 ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissue sections were stained with monoclonal mouse anti-βIII-tubulin primary antibody (Biolegend, Cat. 801202; 1:100 overnight at 4 °C) and goat anti-mouse AF647 (abcam ...
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bioRxiv - Immunology 2023Quote: ... MΦs were generated by incubating bone marrow cells at 37°C with 40 ng/ml M-CSF (Biolegend, San Diego, CA) in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... surface markers were stained with following antibodies at 1:200 dilution at 4°C for 30 min: CD11b- Pacific Blue (BioLegend, 101224), CD45-BV650 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2023Quote: ... centrifuged at 300 rpm for 3 mins followed by incubation at 37°C for 2hrs in the presence of BV421 conjugated anti-CD107a mAb (clone H4A3, Biolegend, #328626). Cell mixtures were then washed in PBS and stained with mAbs to CD56 (FITC or BV605 ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by centrifugation at 4°C followed by staining of cell surface LAMP-1 (CD107a) using FITC-coupled anti-LAMP-1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... Primary antibodies were incubated in 2% BSA in PBS overnight at +4°C (anti-keratin 8 primary antibody 1:1000 [Hybridoma Bank, TROMA-1]; anti-keratin 14 primary antibody 1:1000 [Biolegend, 905301]). Then ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Cancer Biology 2023Quote: ... cell suspensions were stained for 30 min at 4°C in the dark with CD45-BV605 (1:50, BioLegend #304042, RRID:AB_2562106), ITGA6-PeCy7 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Biophysics 2023Quote: ... then cells were stained overnight with DAPI and an Alexa Fluor 647-conjugated anti-cytochrome c antibody (Biolegend, clone 6H2.B4). Stained cells were analyzed using an Attune NxT flow cytometer ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Neuroscience 2023Quote: ... After incubation, cells were centrifuged (350rcf, 7min, 4°C) and resuspended in permeabilization buffer containing Streptavidin (PerCP, Biolegend #405213, 1:600) and antibodies directed against intracellular targets Foxp3 (APC ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed at a dilution of 1:100 for 25 minutes at 4L°C in the presence of mouse Fc block (TruStain FcX™, anti-mouse CD16/32, BioLegend) in PBS containing 5% FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... then incubated for 1 hr at 4°C with the following antibodies: CD26 (DPP4)-fluorescein isothiocyanate (FITC) (Biolegend, 137806; 1:200), anti-mouse ICAM1-phycoerythrin (PE)/Cy7 (Biolegend ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM EDTA then stained with anti-mouse CD31-APC (1:500, Biolegend, 102507), CD45-APC (1:500 ...
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bioRxiv - Immunology 2021Quote: ... at 1:100 dilution in 2% BSA-PBS prior to using streptavidin BV421 (405225, BioLegend) and anti-human VRC01 mAb conjugated to AF488.
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bioRxiv - Immunology 2019Quote: ... Supernatants were collected at 2 hours and 4 hours and analyzed using IL-6 (Biolegend). After removal of supernatant ...
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bioRxiv - Immunology 2019Quote: ... and stained with antigen presenting cells conjugated anti-ERK1/2 pThr202/Thy204 (Biolegend, clone 6B8B69) overnight at 4°C in FOXP3 perm buffer (Biolegend) ...
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bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
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Single cell transcriptomics reveals the effect of PD-L1/TGF-β blockade on the tumor microenvironmentbioRxiv - Genomics 2020Quote: ... resuspended in 1x DAPI solution (in PBS plus 0.5% BSA and 2 mM EDTA; BioLegend) at 5×106 cells/ml ...
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bioRxiv - Immunology 2021Quote: ... Cells were subsequently blocked with 2 μL of TruStain FcX Fc receptor blocking antibody (BioLegend) for 10 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then stained with Brilliant Violent 421-conjugated ms α-EGFR (Biolegend; Table 2). Samples were gated for intact GFP+ cells and geometric mean of fluorescence intensity (geoMFI ...
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bioRxiv - Immunology 2021Quote: ... Lung cells were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...