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Citations for BioLegend :
2001 - 2050 of 2682 citations for TMEM173 Human HEK293 Sumo His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... IL1beta ELISAs were performed using the ELISA MAX™ Deluxe Set Human IL1beta kit (437015, Biolegend) following the protocol provided by the manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-human CD274 (B7-H1, PD-L1) (BioLegend, cat. no. 329707; 1:1000 for WB); mouse anti-DAAM1 (Santa Cruz Bio ...
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bioRxiv - Immunology 2023Quote: ... and cytokine levels determined using the bead-based immunoassays LEGENDplex™ Human Macrophage/ Microglia Panel (Biolegend). The assays were performed in 96-well plates following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and then stained with 1:66 PE-Cy7 anti-human CD71 (TFRC) (BioLegend; Cat. No. 334112) for 30 minutes in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were incubated with Human TruStain FcXTM (FC Receptor Blocking Solution, 1:250, BioLegend catalog #422302) for 5 minutes at room temperature at a 1:250 dilution in DPBS with 1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... and engraftment was assessed regularly by flow cytometric detection of human CD45+ cell percentage (BioLegend, #304011) in peripheral blood starting four weeks post injection (Vogt et al ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated in FC-blocking mix (PBS containing human anti-CD16/CD32/CD64, 1:100 dilution, BioLegend) for 10 min at 4 °C and subsequently stained for surface markers in antibody mix in PBS for 30 min at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies used in the validation include anti-human CD274 antibody (APC, clone 29E.2A3, BioLegend, #329708), anti-human HLA-A,B,C antibody (APC ...
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bioRxiv - Immunology 2024Quote: ... Blast cells (0.1-0.2 x 106) were washed and blocked with Human TruStain Fc blocker (BioLegend) for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice to remove unbound proteins and incubated with APC anti-human IgG (Biolegend) for 30 min on ice and protected from light ...
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bioRxiv - Immunology 2024Quote: ... APC-conjugated mouse IgG2a anti-human SIRP-α Ab (clone 15-414, cat. no. 372109, Biolegend), FITC-conjugated mouse IgG2b anti-human CD32 Ab (clone IV.3 ...
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bioRxiv - Immunology 2024Quote: ... or FITC-labeled mouse anti human CD14 fluorescent antibody (cat. no. 301804; 1:1,000; BioLegend, Inc.) was added into each tube ...
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bioRxiv - Immunology 2024Quote: ... B cells were stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304, clone: 2H7), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... Microglia were stained with 5 ul each of PE-anti human CD45 antibody clone HI30 (Biolegend) and APC-anti human CD11b antibody clone ICRF44 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: 100µl bone marrow were blocked with 2µl Fc Receptor Blocking Solution (Human TruStain FcX™, (422301, Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... we used the following anti-human antibodies for surface-marker staining: anti-CD45 (BV785, clone HI30 – BioLegend), anti-CD3 (Alexa700 ...
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bioRxiv - Cancer Biology 2022Quote: ... Western blot antibodies for the GAPDH loading control were mouse anti-human GAPDH antibody (Biolegend, catalog #649202) and HRP-conjugated anti-mouse IgG (Cell Signaling Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... Purity was assessed by FACS staining with anti-human CD34 BV 421 antibody (clone 561, BioLegend, USA). For long-term storage ...
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bioRxiv - Immunology 2022Quote: ... media was supplemented with 2 μg/ml of mouse IgG1 K anti-human PD-1 antibody (Biolegend) either alone or in combination with 1 μg/ml mouse IgG2 B anti-human TIGIT (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Alexa Fluor® 700 anti-human CD4 (clone RPA-T4; dilution 1:200; Biolegend; Cat no 344808), FITC anti-human CD8a (clone RPA-T8 ...
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bioRxiv - Microbiology 2019Quote: ... Sputum samples were also stained with 1 µg/ml of PE anti-human CD45RO in PBS (BioLegend) for 30 min to stain activated and memory T cells ...
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bioRxiv - Immunology 2019Quote: ... then incubated with 0.5 ml of human TruStain FcX Fc receptor blocking solution (Biolegend, San Diego, CA) for 10 minutes.
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bioRxiv - Immunology 2021Quote: ... cells were washed twice in MACS buffer and incubated with PE–anti-human FC antibody (Biolegend, M1310G05) in MACS buffer for 30 min on ice ...
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bioRxiv - Bioengineering 2021Quote: ... and selected antibodies (APC-labeled mouse anti-human: CD45, CD90, CD105, CD309 and CD147, all from BioLegend) for 30 min in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were stained with 0.1ug TotalSeq-B anti-human Hashtag antibody (Biolegend, 394633, 394635, 394637 or 394639) per sample diluted in 100uL HF buffer for 30 minutes on ice ...
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bioRxiv - Cell Biology 2021Quote: ... Raji B cells were stained for CD19 with PE anti-human CD19 antibody (Biolegend, #392506, clone 4G7) and the top 3% highest CD19-expressing cells (Raji B cells ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: Erythroid progression was followed by immunostaining with phycoerythrin (PE)–conjugated anti-human CD235a (1:100) (116207, BioLegend) and Allophycocyanin (APC)–conjugated anti-CD71 (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: Samples were labelled and imaged with the following primary antibodies: anti-human CD41 (BioLegend Cat# 303702, RRID:AB_314372), anti-human CD34 (BioLegend Cat# 343502 ...
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bioRxiv - Cancer Biology 2022Quote: Flow cytometry analysis or flow cytometry cell sorting was performed using anti-human CD133-APC (BioLegend – 372805) and anti-human CD44-APC (BioLegend – 338805 ...
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bioRxiv - Immunology 2022Quote: ... cells were washed once with PBS and incubated with anti-human antibodies against CD3 (PerCP-Cy5.5, Biolegend), CD56 (BV510 ...
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bioRxiv - Systems Biology 2022Quote: ... washed twice with PermBuffer and stained with anti-human γH2AX AlexaFluor 488 (Biolegend, clone 2F3, cat. #613405) for 1 h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were surface stained with series of antibodies (anti-human CD158 [KIR2DL1/S1/S3/S5]-FITC [BioLegend, Cat#339504] ...
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bioRxiv - Biochemistry 2022Quote: ... and cells labeled with anti-human CD45 (APC, 2D1, eBioscience, 17-9459 and A488, H130, BioLegend, 304019). TCR expression on transfected Jurkat cells was assessed by staining with phycoerythrin (PE)-conjugated mouse anti-human CD3 (BD Pharmingen 555333) ...
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bioRxiv - Cancer Biology 2022Quote: ... was used as the primary antibody and rat anti-human IgG Fc antibody conjugated to APC (BioLegend) was used as the secondary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were resuspended in 25 μL block solution (wash plus 1 in 40 Human TruStain FcX – Biolegend) and incubated for 5 min ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 2 μl of anti-human CD3-AF488 (clone UCHT1, Biolegend) and 1 μl of anti-human CD14-PE (clone 61D3 ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently stained with Brilliant Violet 785™ anti-human CD14 Antibody (1:1000; BioLegend, #301840), APC anti-human CD86 Antibody (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...
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bioRxiv - Immunology 2020Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014), fixed with formalin ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used to stain transfected 293T cells were Brilliant Violet 421-conjugated anti-human CD3 antibody (Biolegend) and phycoerythrin (PE)-conjugated anti-human TCRαβ antibody (BD BioSciences).
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bioRxiv - Immunology 2020Quote: ... We followed manufacturer instructions for the LEGENDplex™ Human Th Panel (13-plex) kit (BioLegend, Cat #740722) in a 96-well V bottom plate ...
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bioRxiv - Cancer Biology 2019Quote: The cells were stained with anti-human monoclonal antibody (mAb) CD4–FITC clone RPA-T4 (BioLegend, CA). For nuclear staining ...
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bioRxiv - Cancer Biology 2019Quote: ... TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6) (BioLegend, CA) and FITC anti-human KRAS antibody (Catalog# ...
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bioRxiv - Cell Biology 2021Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...
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bioRxiv - Cell Biology 2019Quote: ... EPO level was measured in the conditioned media using the LEGEND MAX Human Erythropoietin ELISA kit (Biolegend), according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: ... PSC-derived endothelial cells were sorted by gating for CD31+ (PE anti-human CD31, Biolegend, 1:200) cells.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were isolated by negative selection using MojoSort Human CD4 T Cell Isolation kit (Biolegend). Primary CD4+ T cells were maintained in complete RPMI 1640 medium supplemented with 10 IU/ml IL-2 (Center for AIDS Reagents [CFAR] ...