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Citations for BioLegend :
2001 - 2050 of 7971 citations for 5 Pyrimidinecarbonitrile 1 2R 2 3 dihydroxypropyl 1 2 3 4 tetrahydro 3 methyl 2 4 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-PE-Cy7 and Granzyme B-APC diluted in Perm-Wash buffer (Biolegend). Sample acquisition was performed on a Cytoflex-S (Beckman Coulter ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Immunology 2022Quote: ... Cells were fixed with 4% PFA and stained intracellularly with IFN-γ-BV421 and TNFα-PE diluted in Perm-Wash buffer (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Reaction was stopped by centrifugation at 4°C and externalized LAMP1 (CD107a) was stained using FITC-coupled anti-LAMP1 antibody (clone 1D4B, BioLegend, #121605) for 20 minutes at 4°C in the dark ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was used to mark single cells of DMSO treated mice and TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody (Clone M1/42; 30-F11; 155837; BioLegend) was used for aortic cells of TRAF-STOP treated mice ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 cells were resuspended in PBS + 1% FBS and spun at 300g for 10 minutes at 4°C and then resuspended in 25 μL staining buffer (BioLegend #420201). 2.5 μL human TruStain FcX (BioLegend #42230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation for 10 minutes at 20,000 Xg at 4 °C and VASP was subjected to immunoprecipitation using either His6- specific epitope tag affinity matrix (Biolegend #900501) or control antibody matrix (Novagen #69026) ...
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Bioengineering 2024Quote: ... for 10 min at room temperature and then stained for 30 min at 4°C with the following antibody cocktail: anti-mouse CD45.1 (Biolegend, cat.: 110735), anti-mouse TER-119 (eBiosciences ...
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bioRxiv - Immunology 2024Quote: ... for 4 h and stained for intracellular expression of either IL-4 or FOXP3 after fixation and permeabilization using the Fixation Buffer (BioLegend, # 420801), the Intracellular Staining Perm Wash Buffer (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Splenocytes from naive or tumor-bearing AST;Cre-ERT2 mice were mixed with 2×106 C57BL/6 splenocytes and incubated in RPMI-10 for 4 hours at 37°C in the presence of brefeldin A (Biolegend 420601) and TAG peptide (SAINNYAQKL [0.5 μM] ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Neuroscience 2023Quote: ... 1-16 Antibody (1:000, Clone 6E10, N-terminal, a.a. 1-16, human specific only, 803014 BioLegend, CA, USA); β-Amyloid ...
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bioRxiv - Immunology 2019Quote: ... Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF-400-NA goat anti-IL-1 α, 1:50; Novus Biologicals NB600-408 rabbit anti-collagen I, 1:50; Biolegend 103101 rat anti-CD45, 1:50). The next morning ...
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bioRxiv - Immunology 2021Quote: ... CD71-PE (CY1G4, 1:400), CD38-PE-Cy7 (HIT2, 1:200), CD19-APC (HIB19, 1:200) and Zombie Aqua (all BioLegend). For sorting GC B cells and LNPCs from the lymph node ...
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100, Biolegend; CD140a-APC 1:100 ...
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bioRxiv - Immunology 2021Quote: ... single-cell suspensions were obtained and cells were stained with the following monoclonal antibodies: CD45 (APC-Cy7 #103116 1/200), CD11B (APC #101212, 1/200), Ly6G (PE-Cy7 #127618, 1/100) from Biolegend, Siglec-F (PE #552126 ...
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bioRxiv - Neuroscience 2019Quote: ... The cell pellet was then stained with antibodies to microglial cell surface markers (CD11b-BV650, 1:200 Biolegend, #141723; CD45-BV786, 1:200, BD Biosciences #564225; CX3CR1-FITC, 1:100, Biolegend, #149019 ...
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bioRxiv - Physiology 2019Quote: ... F4/80-PECy7 (1:150, 123114), Ly-6C-BV605 (1:300, 128035) and Ly-6G-FITC (1:300, 127605, all BioLegend). To stain mature leukocytes in the bone marrow ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901]; mouse anti-Pax6, 1:200 [Millipore #MAB5552]; mouse anti-Tuj1, 1:1000 [Biolegend #801201] ...
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bioRxiv - Cell Biology 2021Quote: ... dilution 1/250), anti-mCD71 mAb (RI7217, dilution 1/250) and anti-mTNFα mAb (MP6-XT722, dilution 1/250) from Biolegend; anti-mCD69 mAb (H1.2F3 ...
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bioRxiv - Immunology 2020Quote: ... dilution 1:50), and CD45RO-APC-Cy7 (UCHL1, dilution 1:50), CD127-PE-Cy7 (A019D5, dilution 1:20) (all from BioLegend). FoxP3-APC (eBioscience ...
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bioRxiv - Immunology 2022Quote: ... 1:100), CD62L (clone DREG-56, 1:80), CD66b (clone G10F5, 1:40) and corresponding isotype controls (all from BioLegend). Analysis was done in adherence to guidelines [25].
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD, PDGFRα-APC 1:500 135907 Biolegend, ITGβ4-AF405 1:500 FAB405V Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... CD71-APC (CY1G4, 1:100), CD14-A700 (HCD14, 1:200), and IgM-APC-Cy7 (MHM-88, 1:400) (all BioLegend) diluted in Brilliant Staining buffer (BD Horizon) ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies used were: Vimentin 1:500 (Standard BioTools, #3143027D), CD3 1:250 (Dako, #A0452, Lysozyme 1:400 (Biolegend, #860001), CD11c 1:300 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... clone RM4.5), CD25 (1:500; eBioscience, clone PC61.5), ITGA2 (1:50; eBioscience, Clone DX5) and MCAM (1:100; Biolegend, Clone ME9F1) for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Sections were then stained with primary antibody diluted in 1% BSA in PBS for 1 hour (rat anti CD34 abcam ab8158 1:200 dilution, rat anti CD68 BioLegend 137002 1:400 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... EpCAM-APC/Cy7 (Biolegend, clone G8.8, 0.5 µg ml−1, 1:500), Cd49f-BV421 (Biolegend 313623 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human PD-1 BV785 (1:50, clone EH12.2H7, BioLegend, cat # 329930), Anti-human CD45 BUV395 (1:100 ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µM DRAQ7 and/or 1 µg/ PacificBlue-conjugated Annexin V (BioLegend). For cell death induction in transgenic cell lines ...
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bioRxiv - Pathology 2022Quote: ... BV650-conjugated anti-Gr-1 (Biolegend, clone RB6-8C5, #108441, 1/50), and PE-conjugated anti-Tissue Factor/CD142 (R&D Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse anti-K10 (1:100, BioLegend, 904301, or 1:100, Abcam, ab9026), guinea pig anti-K31 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... PD-1-PE-Cy7 (1:50; clone 29F.1A12; BioLegend Cat. 135216); Cxcr5-APC (1:50 ...
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bioRxiv - Immunology 2020Quote: ... anti-FasL PE-Cy7 (clone NOK-1, Biolegend, Cat. 306418, 1:100), anti-CTLA4 PE (clone L3D10 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti-βIII-tubulin (IF 1:500, WB 1:5,000, Biolegend #801201); rabbit anti-cleaved SNAP25 (IF 1:300) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then resuspended in 1:1 PBS-diluted Fixation Buffer (Biolegend), comprising ~2% PFA ...
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bioRxiv - Developmental Biology 2024Quote: ... mCherry (1:500, Sicgen #AB0040-200) and TUJ1 (1:500, BioLegend #802001). After one hour blocking at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... CD29-Alexa Flour 700 (clone HMβ1-1, Cat# 102218, BioLegend, 1:250), and for hematopoietic markers ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti CD11c (CST #97585, 1:200) or XCR1 (Biolegend 109402, 1:200) antibodies were applied as primary antibodies overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... anti-mouse Podoplanin conjugated with AF488 (1/200, clone PMab-1, Biolegend), anti-mouse CD3 conjugated with AF488 (1/200 ...
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bioRxiv - Immunology 2024Quote: Neutrophil phenotyping panel 1: Ter119-FITC (clone TER-119 Biolegend 1:200), NKp46-FITC (clone 29A1.4 Biolegend 1:200) ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Microbiology 2024Quote: ... The panel of cytokines presented in Fig 5 was measured using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) (BioLegend, Cat# 740809) kit ...
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bioRxiv - Immunology 2021Quote: ... CX3CR1 (Biolegend, 2A9-1), CCR7 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... CD4 (1:50; Biolegend). After washing ...
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bioRxiv - Cell Biology 2020Quote: ... PMP22 (BioLegend, 1:5,000), Cleaved caspase3 and LC3AB I/II (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... 1:500 (BioLegend, 901502); anti-CYTC ...