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Citations for BioLegend :
1951 - 2000 of 2049 citations for Glucose Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: To differentiate between live and dead cells were firstly stained with the Zombie NIR™ Fixable Viability Kit (BioLegend, 423106), the Zombie Yellow™ Fixable Viability Kit (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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bioRxiv - Molecular Biology 2020Quote: ... and LCL A382T TDP-43 mutated patients (LCL-TDP382) were incubated with a vitality dye for 15 minutes (Zombie Violet™ Fixable Viability Kit, BioLegend). Then ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... total CD4+ T cells were first isolated from Foxp3Thy1.1 reporter mice(Liston et al., 2008) by using mouse CD4 T cell isolation kit (Biolegend, catalog 48006), and stained with anti-Thy1.1-PE antibody (eBioscience ...
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bioRxiv - Cancer Biology 2020Quote: ... fixed and stained for intracellular Ki67 following BD Pharmingen’s procedure for FITC BrdU Flow Kit (Cat# 559619) with two modifications: DNase treatment step was omitted and Ki67-AlexaFluor488 (Biolegend #350507) was used instead of anti-BrdU FITC.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...
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bioRxiv - Microbiology 2020Quote: Levels of cytokines and soluble mediators present in plasma of infected mice were quantified using LEGENDplex™ Mouse Inflammatory Panel kit (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Microbiology 2020Quote: ... The levels of murine IL-6 and TNF-α and human IL-6 were measured using ELISA MAX™ kits (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The following ELISA kits were used according to manufacturer recommendations: ELISA Max human ELISA for IL-10 (Biolegend, San Diego, CA), anti-pan human IFN alpha (Interferon Source ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with PBS buffer and stained with a kit of Zombie Aqua™ Fixable Viability (Biolegend, cat.423101) for 20 min at RT ...
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bioRxiv - Immunology 2022Quote: ... True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 (clone MF-14) with CD25 and CD4) was used with FOXP3 Fix/Perm Buffer from BioLegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Microbiology 2022Quote: The level of TNF-α protein expression was evaluated after cell transduction with TPA-TNFα in the cultured medium using human TNFα ELISA kit (Biolegend, UK). First ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: OVA-specific IgE levels were measured using a LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit (BioLegend, San Diego, CA). The plate was washed 4 times with wash buffer followed by addition of Matrix A to standard wells and Assay Buffer to sample wells ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in 200 μl FACS buffer containing 1 μl Zombie NIR fixable dye for 30 min to determine cell viability (Zombie Green™ Fixable Viability Kit, BioLegend). Cells were fixed for 15 min in 200 μl PFA 2 % ...
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bioRxiv - Immunology 2023Quote: ... Ex vivo CD8+ T cells or in vitro stimulated (IVS) cultures were labeled with the Zombie Aqua™ Fixable Viability Kit (BioLegend) to exclude dead cells in all flow cytometry experiments ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Microbiology 2023Quote: ... cells were incubated in 100 µl PBS 1X containing 1.5 μL Zombie NIR Fixable Viability kit (BioLegend, San Diego, CA, USA) at 4°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2023Quote: Proliferation of blood ASC and BM LLPC was evaluated on the basis of bromodeoxyuridine (BrdU) incorporation using a phase-flow BrdU commercial kit (BioLegend, Inc.) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2023Quote: ... CD3+ CAR-T cells were then purified from lymphocytes using MojoSort™ Mouse CD3 T Cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco ...
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bioRxiv - Immunology 2024Quote: ... CORSET8 T cells were purified from the TCR Tg donor mice with the MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2021Quote: The IFN-β levels in cell supernatants and mouse sera were measured with ELISA kits purchased from BioLegend (San Diego, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
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bioRxiv - Cell Biology 2021Quote: ... Dead cell compensation and gating was completed using a 50 % mixture of live and dead mESCs stained with Zombie Aqua™ Fixable Viability Kit (BioLegend 423101) and incubated in the light-tight container at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: To measure the calprotectin/MRP 8/14 from the fecal samples, S100A8/S100A9 ELISA kit rat/mice (#K6936, Immundiagnostik AG, Germany) as well as LEGENplex™ (#740150 or #740446, Biolegend, Germany) mouse inflammation panel (13-plex ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A custom rat multi-analyte LEGENDplex bead-based immunoassay kit was used to examine cytokine concentrations in plasma samples (LEGENDplex; BioLegend, CA, USA) that targeted six cytokines concurrently (IL-6 ...