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Citations for BioLegend :
151 - 200 of 335 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cell-surface stained cells were fixed and permeabilized using True-Nuclear Transcription Factor Buffer Set (BioLegend), incubated with anti-CD5L antibody for 30 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... IL1beta ELISAs were performed using the ELISA MAX™ Deluxe Set Human IL1beta kit (437015, Biolegend) following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... The PBMCs were then fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and IL-1β were carried out using ELISA MAXTM Standard Set (Biolegend, San Diego, CA, USA). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... BrdU-FITC antibody staining for 20 min at RT (BioLegend #364104) and 20 min incubation with PI solution (100 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... TGM-1 (SigmaAldrich®, HPA040171, 1:150) and CD31 (BioLegend®, 303126, 1:100). Secondary antibodies were also applied overnight in a 3% BSA solution in PBS frequently alongside rhodamine-phalloidin and DAPI ...
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bioRxiv - Physiology 2019Quote: ... co-IP experimental conditions using anti-C2CD5 or anti-HA (1:150, Biolegend, #901513) were used as described above.
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with Biotin anti-mouse CD31 (Biolegend; Cat. No. 102503; 1:150), Biotin anti-mouse CD45 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining of Ki67 and p21 were carried out using FoxP3 Fix/Perm Buffer set (Biolegend #421403) following the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Intranuclear staining of Ki-67 was performed with the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were then fixed (TrueNuclear™ TF fixation and permeabilization buffer set, BioLegend, San Diego, CA, USA) at room temperature for 60 min ...
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bioRxiv - Immunology 2022Quote: ... Collected supernatants were assessed for cytokine content using ELISA MAX™ Standard Set Mouse IFN-γ (Biolegend). DCs were analyzed for the expression of CD86 and MHC class II using flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed and permeabilized with a cytokine staining buffer set (Biolegend, Cat#420801 and Cat#421002) for cytokine/chemokine staining and a Foxp3/Transcription Factor Staining Buffer Set (eBioscience/Thermo Fisher Scientific ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Immunology 2023Quote: ... After washing cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) followed by intracellular staining for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using the True-Nuclear Transcription Factor Buffer Set (BioLegend Cat. No. 424401) using the manufacturer’s protocol with overnight fixation/permeabilization.
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bioRxiv - Immunology 2024Quote: ... were stained after cell fixation and permeabilization with True-NuclearTM transcription factor buffer set per protocol (BioLegend). Data were collected on BD LSR Fortessa or Beckman CytoFLEX flow cytometer and analyzed by FlowJo (Tree Star Inc. ...
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bioRxiv - Immunology 2024Quote: ... When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Zombie-Green/Violet fixable viability dyes and Foxp3 buffer set were purchased from Biolegend (San Diego, CA). Lymphocytes were stained for cell surface markers ...
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bioRxiv - Immunology 2024Quote: ... and stained for 15 min at RT with annexin V (Biolegend, 640919).
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bioRxiv - Cell Biology 2023Quote: ... cells were fixed for 20 min in 150 μl 4% paraformaldehyde (BioLegend; cat. no., 420801) at 4 °C in darkness before a 30-min permeabilisation step (eBioscience ...
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bioRxiv - Immunology 2021Quote: ... Staining for FOXP3 followed the instruction provided by True-Nuclear Transcription Factor Buffer Set kit (BioLegend, Cat#: 424401). For apoptosis analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in PBS and fixed/permeabilized with True-Nuclear™ Transcription Factor Buffer Set (Biolegend). Surface (CD3 ...
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bioRxiv - Pathology 2021Quote: ... were coated with purified GM-CSF capturing antibody from ELISA MAX™ Deluxe Set Mouse GM-CSF (Biolegend), left overnight at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... The upper size limit detection threshold was set by fluorescent rainbow particles with mid-range intensity and size 3.0-3.4 µm (Biolegend) resuspended in HBSS ...
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bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... and CV05) were stained with a divergent set of antibodies: CD19-PE 1:50 (clone HIB19, BioLegend, 302207), CD38-PEcy7 1:50 (clone HIT2 ...
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bioRxiv - Neuroscience 2022Quote: ... Panels requiring intracellular staining were then fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (BioLegend), and then stained with intracellular antibodies for 30 minutes at room temperature in the dark before washing ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized and stained for transcription factors using the T rue-Nuclear Transcription Factor Buffer Set (Biolegend, Cat# 424401) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: 1X Fix Concentrate from the True-Nuclear™ Transcription Factor Fixation/Permeabilization Buffer Set (BioLegend, Cat. No. 424401) was added to each sample ...
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bioRxiv - Biophysics 2020Quote: ... astrocytes were labelled 1 hour at RT with anti-GFAP (1:150,644702, BioLegend) for staining GFAP ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 1h at RT using IC fixation/permeabilization buffer (Biolegend) and cells were then incubated for 20 min at RT in permeabilization buffer (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... at RT with either human IgG1 isotype control (Biolegend #403502, 0.47 mg/mL), mAb C7 (0.47 mg/mL) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed twice using 150 µl/well intracellular-staining Perm-Wash buffer (BioLegend, cat. 421002) diluted 1:10 in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... cultures were stained for flow cytometry with anti-CD69-PE/Cy7 (1:150) (BioLegend, Cat.#310912) (Y780/60-A) ...
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2020Quote: ... Intracellular staining was either performed using the True-Nuclear Transcription Factor Buffer Set (Biolegend, San Diego, CA, Cat # 424401) or CytoFix Fixation buffer / FACS Permeabilizing solution 2 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... and Il6) in treated PBMC supernatants were quantified by ELISA using the ELISA MAX Deluxe Set Human CCL2 (Biolegend), ELISA MAX Deluxe Set Human IL8 (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... and TNF-α was measured by enzyme-linked immunosorbent assay (ELISA) per manufacture’s instruction (Biolegend, ELISA MAX Deluxe Set) and CXCL-1/GRO-α was measured using a R&D systems DueSet ELISA kit.
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bioRxiv - Immunology 2023Quote: IL-6 secretion was quantified using an ELISA MAX Deluxe Set Mouse IL-6 according to the manufacturer’s instructions (431304, Biolegend). Total and NP-specific antibody levels and IL-2 secretion were measured by ELISA on half-area 96-well plates (Greiner Bio-One ...
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bioRxiv - Immunology 2023Quote: ... The samples were incubated for 1 hour with fixation buffer from True-Nuclear™ Transcription Factor Buffer Set (Biolegend) and washed with permeabilization buffer from the same set ...
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bioRxiv - Microbiology 2023Quote: ... Detection of IgE from mouse serum was performed by ELISA using ELISA MAX™ Standard Set Mouse IgE (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The supernatants were obtained and IFN-γ and TNF-α concentrations were quantified using ELISA MAX Standard Sets (Biolegend; cat.# 430801 for IFN-γ ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine staining was performed according to manufacturer’s instructions for staining intracellular antigens (eBioscience) or using the True-Nuclear Transcription Factor Buffer Set (BioLegend). Flow cytometric analysis was performed using a FACS FORTESSA and FlowJo softwares (Tree Star ...
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bioRxiv - Immunology 2023Quote: ... and then single-cell suspensions were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Biolegend, #426803) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... FACS gates were set based on CD45 (Alexa Fluor® 594 anti-CD45, 1:20, Biolegend, #368520, clone 2D1), SYTOX Blue and DRAQ5 intensities to isolate live CD45+ and CD45-populations as shown in Supplementary Fig ...
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bioRxiv - Immunology 2024Quote: ... The cells were re-suspended in fixation and permeabilization buffers using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) per manufacturer’s instructions ...