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Citations for BioLegend :
151 - 200 of 3763 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... IL-18 and IFN-γ from human PBMCs were measured by LEGENDplex™ Human Inflammation Panel (Biolegend) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend) [9] ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 5 % human AB serum (Gemini Bio) and 30 U / mL recombinant human IL-2 (BioLegend). The viability of NALM6-GL cells was then assessed by adding 150 ug / mL D-Luciferin (PerkinElmer ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used to stain for IL-4Rα were APC anti-human CD124 (IL-4Rα) (Biolegend, clone G077F6, 1:100) and Live/Dead Fixable Green (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... and IL-1β concentrations were determined with the ELISA MAX™ Deluxe Set Human IL-1β (BioLegend, Cat# 437004) in 96-well half area Costar assay plates (Corning ...
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bioRxiv - Immunology 2022Quote: ... pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend, San Diego, CA). As a negative control ...
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bioRxiv - Cell Biology 2022Quote: ... The single-cell suspension was resuspended in Fc-Block (Human TruStain FcX, BioLegend) and incubated with CD31 microbeads (Miltenyi Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... washed and incubated for 10 min with human Fc TruStain FcX (Biolegend #101320). Cells were stained for surface antigens ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were incubated with Fc Receptor Blocking Solution (Human TruStain FcX, Biolegend) according to the manufacturer’s instructions for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... added with 5 µl of Human TruStain FcX™ Fc Blocking reagent (Biolegend). Cell suspensions were then incubated at 4°C for 10 mins ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with 5 µL Fc-block (Human TruStain FcX, Biolegend 422302) and 1.5 µL of S167-180 HLA class II PE-conjugated tetramer for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 50 µL FACS containing 5 µL human Fc-block (Biolegend, 422302), and blocked for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pre-incubated and blocked with 5μl of Human Fc Receptor Blocking Solution (Biolegend) for 10 mins at room temperature then stained with 5μl/106 cells fluorescent conjugated Brilliant Violet 510™ anti-human CD14 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... or 1:1000 (v/v) Alexafluor 647 conjugated Anti-Human IgG Fc (Biolegend) (for AT118-L Fc Fusion proteins ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc receptors were blocked using Human TruStain FcX™ blocking solution (BioLegend, 422301) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 1x105 cells were incubated with Human TruStain FcX (Fc Receptor Blocking Solution) (Biolegend) for 30 minutes at 4°C in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 50,000 cells were used and blocked with Human TruStain FcX (Fc block, BioLegend) in FACS buffer (0.5% BSA (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... and Human TruStain FcX Fc receptor blocking solution (no. 422302, BioLegend; 1:400) for 5 minutes in FACS buffer (PBS containing 5% FBS and 1 mM EDTA ...
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bioRxiv - Immunology 2023Quote: ... and PE/Cy7-conjugated secondary anti-Human IgG Fc antibody (Clone: HP6017, Biolegend) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... washed twice HBSS containing 2% FCS and incubated with Human Trustain Fcx (Biolegend) for 20 min on ice ...
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bioRxiv - Cell Biology 2024Quote: ... All samples were blocked with Human TruStain FcX (Fc Receptor Blocking Solution; Biolegend) on ice for 10 min before immunostaining with vimentin rabbit mAb conjugated with Alexa fluor 647 (clone ...
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bioRxiv - Bioengineering 2024Quote: Quantification of secreted cytokines was conducted using the 13-plex LEGENDplex Human Macrophage/Microglia panel (BioLegend) designed for the identification of IL-12p70 ...
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bioRxiv - Biochemistry 2021Quote: ... The assay was performed using human IL-8 ELISA Max Deluxe (BioLegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... 100ng/ml of recombinant human (rh) IL-8 (Biolegend, San Diego, CA) were added to the apical side of the enteroid monolayers (76) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL human recombinant IL-3 (BioLegend, San Diego, CA, USA) and antibiotics ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were harvested 24 hours later and IL-2 concentrations were determined using serially diluted supernatants and the human IL-2 ELISA MAX Deluxe kit (BioLegend) adapted to 384-well plate format using one quarter of the recommended reaction volumes ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Immunology 2019Quote: ... and stained with an AF647-labeled anti-human IgG Fc antibody (Biolegend, catalog 409320). Cells were then analyzed by flow cytometry on LSRFortessa analyzer (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in 1:50 dilution of human FC blocking solution (BioLegend; #422302) and incubated on ice for 10 min ...
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bioRxiv - Immunology 2021Quote: ... non-specific binding was blocked using Fc Receptor blocking solution (Human TruStain FcX, BioLegend) and cells were stained with antibodies in PBS supplemented with 2% hiFCS for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: After blockade of Fc-receptors using Human TruStain FcX™ (BioLegend, London, United Kingdom), 5*106 PBMCs were stained with fluorescently labelled antibodies in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... re-suspended and labelled with Fc block (Human TruStain FcX™; Biolegend, London, UK) for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were coated with recombinant human E-cadherin-Fc chimera (BioLegend #BLG-779904-25ug) to reduce colony formation and thereby limit the potential impact of paracrine and direct cell signaling in regulating gene expression of related cells 50 ...
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bioRxiv - Immunology 2022Quote: ... Fc-receptors were blocked by adding 2.5 μL Human TruStain™ FcX (BioLegend, #101320) per 50 μL of 200 000 cells ...
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then stained with anti-human IgG Fc-APC (Biolegend, Cat#366906) on ice for 30 min and washed with PBS twice ...
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bioRxiv - Developmental Biology 2023Quote: ... Following 5 µl of the Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend, #422302) were added to the sample and incubated for 10 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: ... Non-specific bindings were blocked using Human TruStain FcX (Fc Receptor Blocking Solution, BioLegend) for 10 min on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... We followed manufacturer instructions for the LEGENDplex™ Human Th Panel (13-plex) kit (BioLegend, Cat #740722) in a 96-well V bottom plate ...
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bioRxiv - Immunology 2023Quote: ... Human Th17 differentiation assays were performed similarly except the concentrations of recombinant human IL-6 (30 ng/ml, BioLegend), TGF- β1 (2.25ng/mL) ...
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bioRxiv - Microbiology 2020Quote: ... The levels of murine IL-6 and TNF-α and human IL-6 were measured using ELISA MAX™ kits (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... and 20 ng/mL recombinant human IL-3 (BioLegend; San Diego, CA, USA) to determine cellular distribution ...
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bioRxiv - Molecular Biology 2021Quote: ... PE anti-human IFNγ and anti-IL-6 antibodies were purchased from BioLegend. Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and IL-22 using the LEGENDplex Human Th Cytokine (Biolegend, San Diego, CA). Cells from 3-day long re-stimulated cultures were used for evaluating expression of ...
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bioRxiv - Immunology 2023Quote: ... plasma was tested with human IL-6 ELISA MAX Deluxe Set from Biolegend.
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bioRxiv - Microbiology 2023Quote: ... Quantification was conducted using ELISA Max Deluxe Set Human IL-1β kit (Biolegend) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2023Quote: Cytokines were quantified using the LEGENDplex Human CD8/NK Panel 13-plex (Cat.No # 740267 Lot B330268) from Biolegend. This is a bead-based immunoassay able to quantify the concentration of different analytes in co-culture supernatants ...
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bioRxiv - Immunology 2023Quote: Cytokine analysis from co-culture supernatants was performed with Human CD8/NK Panel (13-plex) Cat#741065 (BioLegend) following the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.