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Citations for BioLegend :
151 - 200 of 241 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... then fixed and permeabilized for intranuclear staining for 1 h at 25 °C with True Nuclear fixation buffer (BioLegend), washed twice with permeabilization/wash buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 h at RT and stained with two staining panels: (1) anti-CD3 (clone UCHT1; #300415, BioLegend, USA) and anti-CD14 (clone HCD14 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained with MHC-I (H-2Kd/Dd-Alexa-647, BioLegend, clone 34-1-25, 1:200 dilution) or MHC-II (I-A/I-E-APC-Cy7 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 8 h at 37 °C followed by staining with a fixation/permeabilization buffer according to the manufacturer’s protocol (BioLegend). After washing ...
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bioRxiv - Immunology 2023Quote: ... fibroblasts and in vitro differentiated plasmablasts (generally <10% frequency) were removed using biotinylated anti-H-2Kd (Biolegend, cat: 116604) and anti-CD138 (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and their working dilutions included SMI-32 neurofilament H non-phosphorylated (SMI-32; mouse, Biolegend; 1:1000), Ankyrin-G (Guinea Pig ...
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bioRxiv - Cancer Biology 2024Quote: ... and labeled with anti-MHC-I fluorescent antibody (anti-mouse MHCH-2kd/H-2Dd, BioLegend, Clone: 34-1-25). Cells were then washed with PBS and analyzed with the NovoCyte Advanteon Flow Cytometer (Agilent ...
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bioRxiv - Immunology 2021Quote: ... R&D), CD64 (X54-5/7.1) and MHC class II (M5/114.15.2, eBioscience) were purchased from BioLegend unless otherwise stated ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with 7-Amino Actinomycin D viability staining solution (7-AAD, BioLegend, San Diego, CA). The suspensions were incubated for 45 min on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... and homogenized lung were measured by ELISA according to the manufacturer’s instructions (Biolegend or R&D Systems). The levels of PGE2 were measured at the serum or the peritoneal lavage fluid of septic mice ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Immunology 2024Quote: ... and APC anti-ligand (B7-H6: R&D, clone 875001; CD48: Biolegend, clone; BJ40; Nectin-2: Biolegend, clone TX31 ...
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bioRxiv - Immunology 2021Quote: ... or PE-conjugated anti-human CD47 mAb CC2C6 (with cross-reactivity to pig CD47 and thus, referred to as anti-h/pCD47; Biolegend). The level of cell surface CD47 is expressed as median fluorescent intensity (MFI) ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... activated T-cells were treated with HDACI as described before and after 72 h total protein acetylation of the cells was determined by flow cytometry using PE-coupled anti-Acetylated Lysine Antibody (Biolegend). Cells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: ... at 2:1 ratio for 1 h at 4C to ensure maximum staining quality before surface staining with CD20-FITC (2H7, Biolegend) for an additional 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... cells isolated from the lungs at 18 and 48 h post co-infection were also stained with either Ly6G clone 1A8 or RB6 clone RB6-8C5 (BioLegend) antibodies to confirm cell depletion ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... cells were collected by scraping and stained with APC-conjugated anti-mouse H-2Kb bound to SIINFEKL antibody (141606, BioLegend) and PerCP-Cy5.5-conjugated anti-mouse CD86 antibody (105027 ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
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bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
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bioRxiv - Immunology 2024Quote: ... the cells were first gated for singlets (FSC-H vs. FSC-A) and further analyzed for their uptake of the Live/Dead Zombi violet stain (Biolegend) to determine live versus dead cells in CD3+CD4+ ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human GPA primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin-streptomycin for 16 h before exposure to recombinant mouse IL-6 (rIL-6) at 50 ng/ml (575702, BioLegend) or vehicle (PBS ...
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bioRxiv - Molecular Biology 2024Quote: IL-6 KO mice with cast immobilization for 24 h were subjected to intraperitoneal administration of 400 ng of rIL-6 (575702, BioLegend) in saline or of saline alone ...
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bioRxiv - Immunology 2024Quote: ... and 2.5 mM HEPES into 6-well plates pre-coated for more than 1 h with 5.4 µg of Ultra-LEAFTM purified anti-mouse CD3e antibody (clone 145-2C11, Biolegend, # 100340) and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated O/N at 4°C with primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), hnRNP A2/B1 (sc-374053 ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and cytokines quantified by ELISA according to manufacturers’ instructions (R&D Systems, Biolegend of Invitrogen), except antibody and sample volumes were halved.
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... After 48 h incubation, cells were collected and stained with anti-CD3-FITC (BioLengend, 300406) and anti-CD69-APC (BioLegend, 310910) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Bioengineering 2020Quote: ... anti-Pax7 (mouse monoclonal, DHSB, diluted 1:4) and anti-Neurofilament H phospho (rabbit polyclonal, BioLegend # PRB-573C, diluted 1:400). As secondary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Systems Biology 2022Quote: ... membranes were blocked with 5% non-fat milk at room temperature (RT) for 1 h and probed with primary RpoC (β prime subunit of RNAP) antibodies at 1:2000 dilutions (Biolegend) at 4 °C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were isolated and stimulated for 48 h with 3 μg/ml plate-bound anti-mouse CD3 antibody (100340, BioLegend, USA) and 2 μg/ml soluble anti-mouse CD28 antibody (102116 ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... was first applied for 1 h followed by staining with PE anti-streptavidin (1:200, Biolegend, cat. no. 410503, clone 3A20.2) and anti-c-myc-FITC (1:50 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells transfected with pMAX-GFP and either pcDNA3.1-Rai or empty vector were added in a 10:1 ratio and incubated for 4 h in presence of APC anti-LAMP1 antibody (clone H4A3, Biolegend #328620). Monensin (2mM ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human CD41/CD61 primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...