Dendritic Cell Specific Transmembrane Protein DCSTAMP Antibody Biotin citations for BioLegend

Quote: ... thereafter Biotin-conjugated antibodies were detected with Phycoerythrin (PE)/Cy7 Streptavidin (Biolegend, 405206: 1:300), and subsequently stained for EpCAM+ FITC (Biolegend ...

Endothelial cells are a key target of IFN-g during response to combined PD-1/CTLA-4 ICB treatment in a mouse model of bladder cancer

Sharon L. Freshour, et al., bioRxiv - Cancer Biology 2023

Quote: ... Biotin anti-mouse CD119 (IFN-γ R α chain) Antibody (BioLegend, catalog #112803, clone 2E2), PE Streptavidin (BioLegend ...

Targeting GFPT2 to reinvigorate immunotherapy in EGFR-mutated NSCLC

Luyao Ao, et al., bioRxiv - Cancer Biology 2024

Quote: ... Non-specific antibody binding was precluded with Human TruStain FcX™ (422301, Biolegend, USA). A suite of fluorochrome-conjugated monoclonal antibodies was then employed to identify surface antigens ...

Intra-Articular Delivery of an Indoleamine 2,3-Dioxygenase Galectin-3 Fusion Protein for Osteoarthritis Treatment in Male Lewis Rats

Brittany D. Partain, et al., bioRxiv - Bioengineering 2021

Quote: ... IL6 was quantified using an ELISA developed from an antibody pair (biotin anti-rat IL6 detection antibody, cat. #517703, and purified anti-rat IL6 coating antibody, cat. #517701, Biolegend, San Diego, CA, USA). For this ELISA ...

CD163 and Tim-4 identify resident intestinal macrophages across sub-tissular regions that are spatially regulated by TGF-β

Ian E. Prise, et al., bioRxiv - Immunology 2023

Quote: ... Tissues were blocked for endogenous biotin using an avidin biotin blocking kit (BioLegend), according to the manufacturer’s instructions and incubated overnight at 40C with one of the following primary antibodies ...

Localized APP pathology in the hippocampus is sufficient to result in progressive disorganization of the timing of neuronal firing patterns

Silvia Viana da Silva, et al., bioRxiv - Neuroscience 2022

Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis

Dennis Ma, et al., bioRxiv - Cancer Biology 2020

Quote: ... We split our single cell suspension in half and stained half of the uncultured HCI010 and HCI002 cells for FACS using fluorescent labeled antibodies for human specific CD298 (Biolegend, cat. no. 341704) and mouse-specific MHC-I (eBioscience ...

Light-controlled elimination of PD-L1+ cells

Judith Jing Wen Wong, Pål Kristian Selbo, bioRxiv - Cancer Biology 2021

Quote: ... The cells were then incubated with biotin-anti-human CD274/PD-L1 (BioLegend) on ice for 20 min ...

SARS-CoV-2 variant B.1.1.7 caused HLA-A2⁺ CD8⁺ T cell epitope mutations for impaired cellular immune response

Chanchan Xiao, et al., bioRxiv - Immunology 2021

Quote: ... tetramer specific CD8+ T cells and apoptosis marker Annexin V-APC (BioLegend) on T2A2 cells were evaluated after 16 hours and 7 days ...

Maximizing the Anti-tumor Potential of Immune Checkpoint Blockade through Modulation of Myeloid-specific CXCL16 and STAT1 Signaling

Bhavana Palakurthi, et al., bioRxiv - Cancer Biology 2022

Quote: ... Myeloid cell specific markers used were: BV421 anti-mouse CD86 (BioLegend,105031), BV570 anti-mouse CD11c (BioLegend,117331 ...

Maximizing the Anti-tumor Potential of Immune Checkpoint Blockade through Modulation of Myeloid-specific CXCL16 and STAT1 Signaling

Bhavana Palakurthi, et al., bioRxiv - Cancer Biology 2022

Quote: ... T cell specific markers used were BV 421 PD-1 (BioLegend,135217), BV510 CD69 (BioLegend,104531) ...

Steady-state, therapeutic, and helminth-induced IL-4 compromise protective CD8 T cell bystander activation

Nicholas J Maurice, et al., bioRxiv - Immunology 2024

Quote: ... we used mouse-specific CD8 T cell negative isolation “MojoSort” kits (BioLegend). We plated 0.5 to 1 x 106 CD8 T cells per well in 96-well V-bottom tissue culture plates ...

Identification, crystallization and epitope determination of public TCR shared and expanded in COVID-19 patients

Xiuyuan Lu, et al., bioRxiv - Immunology 2021

Quote: ... anti-rat CD2 (OX-34) and biotin anti-phycoerythrin (PE)(PE001) antibodies were purchased from BioLegend. Rat IgG2b κ Isotype Control (eB149/10H5 ...

Thymic epithelial organoids mediate T cell development

Tania Hübscher, et al., bioRxiv - Developmental Biology 2024

Quote: ... The following primary and secondary antibodies were used: MHCII-Biotin (BioLegend, Catalog No. 107603, 1/200), UEA1 (Vector Laboratories Catalog No ...

Cell state and transcription factor modulation during extended ex vivo CD8⁺ T-cell expansion

Yuan Lui, et al., bioRxiv - Immunology 2024

Quote: ... A final concentration of 0.5 μg/ml biotin-conjugated mCD45 antibody (clone #30-F11, BioLegend: 103103) was added to the cells on ice ...

Borrelia burgdorferi Secretes c-di-AMP as an Extracellular Pathogen-Associated Molecular Pattern to Elicit Type I Interferon Responses in Mammalian Hosts

Raj Priya, et al., bioRxiv - Immunology 2024

Quote: ... plates were incubated with biotin-conjugated secondary antibody for 1 hour at RT (BioLegend, catalog 508105). The plates were washed with PBS-T and incubated with Avidin-peroxidase for 30 minutes at RT (Sigma-Aldrich ...

Improving germline transmission efficiency in chimeric chickens using a multi-stage injection approach

Danial Naseri, et al., bioRxiv - Developmental Biology 2021

Quote: ... Specific primary antibodies were monoclonal purified anti-GFP (Biolegend, San Diego, CA, USA; 1:1000) and monoclonal anti-ß-actin (Clone AC-74 ...

Hypoxic memory of tumor intrinsic type I interferon suppression promotes breast cancer metastasis

Oihana Iriondo, et al., bioRxiv - Cancer Biology 2023

Quote: ... for 15 minutes and incubated with the human-specific anti-CD298-APC antibody (Biolegend, 341706) for 30 minutes ...

Antigen presentation by discrete class I molecules on brain endothelium dynamically regulates T-cell mediated neuropathology in experimental cerebral malaria

CE Fain, et al., bioRxiv - Neuroscience 2022

Quote: ... The antibodies that are specific for surface markers included CD103 Brilliant Violet 421 (121422; BioLegend), NK1.1 Pac Blue (108722 ...

Breast cancer stem cell-derived tumors escape from γδ T cell immunosurveillance in vivo by modulating γδ T cell ligands

Katrin Raute, et al., bioRxiv - Immunology 2022

Quote: α-TCRVδ1-Biotin (clone REA173) from Miltenyi and α-TCRVδ2-Biotin (clone B6) from BioLegend were combined with the antibody solution provided in the MACS TCRγ/δ+ T Cell Isolation Kit to separate Vδ2+ and Vδ1+ cells from γδ T cell expansion cultures ...

Impaired iron recycling from erythrocytes is an early hallmark of aging

Patryk Slusarczyk, et al., bioRxiv - Physiology 2022

Quote: ... anti-CD3 (Biotin, BioLegend, 100243), anti-mouse Ly-6C (Biotin ...

Adaptive immunity is required for durable responses to alectinib in murine models of EML4-ALK lung cancer

Emily K. Kleczko, et al., bioRxiv - Cancer Biology 2022

Quote: ... CD103-Biotin (Clone 2E7; BioLegend), 171Yb-CD44 (Clone IM7 ...

A specialized bone marrow microenvironment for fetal haematopoiesis

Yang Liu, et al., bioRxiv - Developmental Biology 2021

Quote: ... and CD71-biotin (Biolegend, 113803) in combination with anti-Biotin UltraPure MicroBeads (Miltenyi Biotec ...

The scaffold-dependent function of RIPK1 in dendritic cells promotes injury-induced colitis

Kenta Moriwaki, et al., bioRxiv - Immunology 2021

Quote: ... biotin-conjugated CD103 (BioLegend, 2E7), Pacific blue-conjugated CD45.2 (BioLegend ...