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Citations for BioLegend :
151 - 200 of 499 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... total lymphocytes were counted and Naïve B cells were isolated using Mojosort™ Naïve B cell isolation beads (Biolegend 480068) or Naïve B cell Isolation Kit II (Miltenyi 130-091-150 ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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bioRxiv - Immunology 2023Quote: NK cells were isolated from frozen PBMCs by negative selection using the MojoSort™ Human NK Cell Isolation Kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... neutrophils were isolated by negative sorting by magnetic selection using the Mojosort neutrophil isolation kit according to the suggested protocol (Cat# 480058-BioLegend). Purity was checked by flow cytometry using CD11b and Ly6G surface staining ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from the spleen of OT- I mice using the MojosortTM Mouse CD8+ T cell isolation kit (Biolegend) and stained with 5µM CellTrace Violet (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Bioengineering 2023Quote: ... Mo were then isolated from the suspension of differentiated BMC using a MojoSort™ Mouse Monocyte Isolation Kit (BioLegend #480154). The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207) ...
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bioRxiv - Immunology 2024Quote: ... The Mojosort monocyte isolation kit was used to isolate monocytes by negative selection according to the manufacture’s protocol (Biolegend, #480059) and cells were resuspended in RPMI supplemented to with 100ng/mL M-CSF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4 and CD8 T cells were isolated from the spleen and peripheral LNs of C57BL/6 mice using the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kit from BioLegend and irradiated with 254 nm-UV light (600 mJ/cm2 using a Stratalinker 2400) ...
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bioRxiv - Immunology 2024Quote: ... a single cell suspension was prepared and then cells were cryopreserved as described previously.43 Naive B cells were purified by magnetic bead depletion using the Mojosort Human Naive B Cell Isolation kit (BioLegend) according to manufacturer’s instructions but with some additional modifications as previously described.2 Following purifications ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were negatively isolated from spleen and peripheral LNs of donor mice using the MojosortTM Mouse CD8+ T cell Isolation Kit (BioLegend). T cell purity was confirmed to be > 95% by flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from splenocytes of BALB/c mice using a MojoSort mouse CD3 T cell isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and the naïve T cells were harvested using a MojoSort™ mouse CD8+ T cell isolation kit (#480008, Biolegend, US) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells were magnetically purified from the spleens of indicated donor mice by negative selection using MojoSort Mouse CD8 T cell Isolation kit (Biolegend) to >95% purity ...
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bioRxiv - Immunology 2024Quote: ... Autologous CD8+ T cells were isolated from PBMCs using EasySep human CD8+ T cell isolation kit (TEMCELL, Cat# 17953) and stained with 2 μM CFSE (BioLegend) in PBS at r.t ...
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bioRxiv - Immunology 2024Quote: ... Isolation purity was verified by flow cytometry using antibodies against neutrophil surface markers anti-human CD11b (Alexa Fluor 594) (Biolegend) and anti-human CD15 (SSEA-1)(Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated from the spleen and lymph nodes of FVB/N mice using the MojoSort Mouse CD8 T cell isolation kit (BioLegend) as per the manufacturer’s protocol for negative selection strategy ...
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bioRxiv - Immunology 2024Quote: CD4+YFP+ Treg cells from Foxp3cre and Foxp3cre Cxxc1fl/fl mice were enriched using the Mouse CD4 T Cell Isolation Kit (480005; Biolegend) and then sorted using the BD Aria II flow cytometer ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... MaxiSorpTM ELISA plates (Biolegend cat# 50-712-278) were coated with human ACE-2 Fc chimera protein (R&D Systems cat# 10544-ZN ...
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bioRxiv - Microbiology 2023Quote: ... the plates were incubated with TMB substrate (BioLegend) for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... AMs were lifted from plates using Accutase (BioLegend) and seeded for experiments.
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Immunology 2023Quote: ... CD3+ CAR-T cells were then purified from lymphocytes using MojoSort™ Mouse CD3 T Cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Naive CD4+ cells were isolated by negative selection using the MojoSort CD4+ naïve T-cell isolation kit from Biolegend (Catalog 480040) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend) in alignment with the manufacturer’s guidelines.
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bioRxiv - Immunology 2024Quote: ... Treg cells were co-transferred into Rag1-/- recipient mice with Teff cells (purified by anti-PE magnetic beads system and followed by CD3 T cell isolation, Biolegend # 480024)
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend, 10ug/ml) or with IL-12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: For bead-based isolation of human CCR5+ and CCR5− cPMN were sorted using anti-APC MojoSort™ nanobeads (Biolegend, San Diego, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: CD4+ cells were isolated from pooled splenocytes and lymph node cells with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from in vitro treated CAR-T cells using Mojosort™ Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). After washing cells with PBS twice ...