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Citations for BioLegend :
151 - 200 of 2637 citations for CD32 Human R167 HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Purified anti-His tag mouse antibody was from BioLegend (USA). Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... incubating the cells with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2019Quote: ... They were blocked for 1 hour at room temperature in 2% donkey serum and CD16/CD32 (Biolegend, Clone 93, 1:200). Samples were incubated overnight at 4°C in primary antibody diluted in blocking buffer (R&D AF771 goat anti-IL-1R ...
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bioRxiv - Immunology 2021Quote: ... cells were initially blocked with anti-mouse Fcγ III/II receptor (CD16/CD32) antibodies and dead cells excluded by staining with a fixable live/dead cell-exclusion dye (BioLegend, USA). Then ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Immunology 2024Quote: The following antibodies were used: unconjugated anti-CD16/CD32 (1 μg per 1×106 cells; clone 93; BioLegend Cat. No. 101320), H2-Dd-FITC (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Immunology 2021Quote: ... Cell suspensions were resuspended in FACS buffer (PBS, 1% FBS, 1 mM EDTA, 0.02% NaN3) and treated with anti-CD16/CD32 (clone 93, Biolegend 101302, 1:100) for 20 min on ice before staining.
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Fc receptors were blocked with anti-mouse CD16/CD32 antibodies and stained with fluorophore or biotin-conjugated anti-mouse antibodies (BD Pharmingen, Biolegend, Tonbo or eBioscience) to detect B220 (RA3-6B2) ...
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bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
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bioRxiv - Immunology 2019Quote: Cells were incubated for 10 minutes at 4°C with 10μg/ml of purified rat anti-mouse CD16/CD32 (Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Filtered single cell suspensions were blocked (anti-mouse CD16 / CD32) and stained for live/dead discrimination (Zombie Aqua, BioLegend, or EMA, Thermo Fisher) prior to staining with fluorophore-conjugated antibodies (30 min ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.
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bioRxiv - Immunology 2022Quote: ... using human (BioLegend) and murine (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... anti-human (Biolegend): GZMB-APC (Cat.372203) ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 647 anti-human human CD3 Antibody (BioLegend, 300416), Biotin anti-human CD3 Antibody (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... The blocking of nonspecific binding was induced in the cell suspensions via incubation for 10 min at 4°C with anti–CD16/CD32 antibody (cat # 101320, BioLegend, clone 93, 5 ng/μl). This was followed by staining for 30 min at 4°C with the appropriate antibodies ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors (FcRs) were blocked using Human TruStain FcX™ (BioLegend). In samples stained with multiple BD Horizon Brilliant reagents ...
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bioRxiv - Cancer Biology 2023Quote: Human PBMCs were treated with Fc blocking solution (Human TruStain FcX, Biolegend) and stained with the following fluorescent antibodies from Biolegend (unless otherwise stated) ...
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bioRxiv - Immunology 2021Quote: ... human CD4-APC (Biolegend), human FoxP3-APC (eBioscience) ...
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bioRxiv - Neuroscience 2021Quote: ... Human TruStain FcXTM (Biolegend). Protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD3 (BioLegend, #317324), mouse CD45 (BioLegend #103130) ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), human CD25 (BioLegend #302606) ...
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bioRxiv - Cancer Biology 2023Quote: ... human FOXP3 (BioLegend #320116), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab (clone ALULA ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD45 (BioLegend #304014), Zombie NIR™ Fixable Viability Kit (BioLegend #423106 ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD25 (BioLegend #302606), human FOXP3 (BioLegend #320116) ...
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bioRxiv - Cancer Biology 2023Quote: ... human CD8 (BioLegend #344712), human CD45 (BioLegend #304014) ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Microbiology 2023Quote: ... Human TruStain FcX (BioLegend) was added at a 1:10 dilution ...
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bioRxiv - Bioengineering 2024Quote: ... Recombinant human FcγIIa (Biolegend), FcγRIIb (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Human TruStain FcX (BioLegend) was added at 1:20 to block Fc receptors for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... human TGFβ1 (580706; BioLegend), human IL-12 (219-IL;R&D Sys-tems) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend, 652502) and polyclonal α-CsgA-antibody and α-TasA-antibody.
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bioRxiv - Bioengineering 2022Quote: ... Confirmation of successful bead conjugation was determined by staining with anti-His Tag (Biolegend) and flow cytometry analysis ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The Western blot membranes were stained with HRP anti-His tag antibody (Biolegend, #652504) diluted 1:2,000 in 0.5% (w/v ...
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bioRxiv - Immunology 2021Quote: ... Human Fc receptors were blocked using human TruStain FcXTM (BioLegend Cat# 422302, RRID:AB_2818986).
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bioRxiv - Bioengineering 2021Quote: ... containing 1/100 diluted anti-human CD3 or anti-human TCRα/β (Biolegend), both targeting the TCR complex ...
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... A549 were blocked with human Fc receptor blocking solution (Human TruStain FcX, Biolegend) for 10 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD57-PB (BioLegend, Cat#322316], anti-human CD16-V500 [BD Biosciences, Cat#561314], anti-human CD69-PE-Cy7 [BioLegend, Cat#310190]). 5×105 to 1×106 cells were re-suspended in residual buffer and antibody panels added and incubated on ice for 20 to 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: Human PBMCs were labeled with Alexa 647 anti-human CD11c (301620, clone 3.9, BioLegend), anti-human HLA-DR (Class III ...
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bioRxiv - Physiology 2021Quote: Immunoglobulins from human BALF were measured with the LEGENDplexTM Human Immunoglobulin Isotyping panel (BioLegend), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... Human ucOCN measurements were performed using a commercially available human ucOCN ELISA (BioLegend, 446707) (Lacombe et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... Human leukemia cells were labelled with a FITC anti-human CD45 antibody (368508; BioLegend) then sorted using a BD FACS Aria II Cell Sorter.
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bioRxiv - Immunology 2023Quote: ... samples were stained with anti-human HLA-ABC and anti-human CD3PE (BioLegend, 317308) at RT in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... followed by the staining with anti-His-APC (1:20, clone J095G46, BioLegend cat# 362605) with Fc block (1:20 ...