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Citations for BioLegend :
1901 - 1950 of 3674 citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... staining was performed for 30 minutes at 4 C with both 70 nM AlexaFluor647-conjugated HER2 and 1ng/μL anti-DYKDDDDK (anti-FLAG) Tag Antibody-PE (Biolegend, 637310). The 5A12 library was first incubated with either 0.5 nM VEGF (Acro ...
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bioRxiv - Cancer Biology 2019Quote: ... supernatants were harvested and stored at −80°C until the amount of liberated cytokine could be quantified by ELISA MAXTM Human IL-2 or Human IFN-g detection kits (BioLegend #431801 and #430101).
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor 488 anti-human CD206 and PE/Cy7 anti-human Interleukin-10 (IL-10) were used for analysis (all from BioLegend, San Diego, CA). The samples were run on an LSR Fortessa cell analyzer from BD (Becton Dickinson ...
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bioRxiv - Immunology 2019Quote: ... mouse anti-human CD247 (CD3ζ) mAb (clone 6B10.2) and mouse anti-human EGFR antibody (clone AY13) were purchased from Biolegend (San Diego, CA, USA). Anti-human CD32 mAb (clone 8.26 ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells pooled from several individuals were stained with TotalSeq-C custom human panel together with Human TruStain FcX (Biolegend. Cat. No. 422301) on ice for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell, cat. no. 17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, cat. no. 480082) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... For surface antigen staining cells were incubated for 20 min with FITC-labelled anti-human CD44 (eBioscience, clone IM7) and PE-labelled anti-human CD82 (Biolegend, clone ASL-24) antibodies and washed twice with 1% BSA staining solution ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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Optimized immunoglobulin knock-ins using Cas9 reveal peritoneal B cell lineage relationships in vivobioRxiv - Immunology 2021Quote: Cells were incubated with anti-CD16/32 (Fc block) prior to staining with the following fluorophore or biotin conjugated monoclonal antibodies purchased from Biolegend, eBioscience ...
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked for 10 min on ice following addition of 10 μL Fc blocking solution (BioLegend, cat. no. 422301), 4 μL of a 1% dextran sulfate solution (Research Products International ...
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bioRxiv - Immunology 2022Quote: ... T cells were washed in PBS/1% FCS and stained using the following mAbs in appropriate combinations: anti-OX40-PE-Cy7 (BioLegend), anti-GITR- BV421 (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: The myelin-depleted single-cell suspension obtained above was incubated (15 min on ice) with anti-mouse CD16/CD32 antibody to block Fc receptors and subsequently incubated with anti-CD11b PEcy7 (Biolegend), anti-CD45 APC (Biolegend) ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was discarded and the cell pellets were resuspended in 10% FCS in PBS containing Alexafluor647 anti-Ki67 (Biolegend; 1:200 in binding buffer ...
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... cells were washed once in in 1ml of PBS-1%FCS (8’, 350rcf).Cells were then separately labeled with hashtag antibodies (Biolegend TotalSeq-A antibodies ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2021Quote: ... Resulting single cell suspensions of splenocytes or bone marrow cells were Fc blocked with Trustain FcX (anti-mouse CD16/32 clone 93-Biolegend) and stained with combinations of the following antibodies ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and stained for 30min at 4°C in the dark with Fc block diluted 1:200 and subsets of the following antibodies: anti-CD103 (2E7, Biolegend), anti-CD11b (M1/70 ...
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bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
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bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Immunology 2024Quote: ... The pelleted cells were resuspended in FACS buffer (PBS + 1% bovine serum albumin + 2 mM EDTA + 0.1% sodium azide) containing 1:100 dilution of Fc Block and a 1:250 dilution of Zombie Violet dye (423113; Biolegend). After incubation at 4°C for 15 minutes ...
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bioRxiv - Immunology 2024Quote: ... splenocytes (2*107/mL in IMDM medium containing 2% FCS) were simultaneously stained with anti-B220-Alexa647 (clone RA3-6B2 Biolegend) and loaded with 5 μM Indo-1 acetoxymethyl (Indo1-AM ...
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bioRxiv - Immunology 2024Quote: ... 0.1−0.5×106 cells were incubated for 15 min at 4 °C with anti-mouse Fc-block CD16/32 antibody (clone 93, 1:100) and True-Stain Monocyte Blocker (1:100) (both from Biolegend), in 100 µl FACS staining buffer (1.5% FBS and 0.5% BSA in PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... The PE anti-human CD274/PD-L1 (Biolegend, catalog# 329706) antibody and the isotype control antibody (PE Mouse IgG2b ...
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bioRxiv - Cancer Biology 2021Quote: ... APC anti-human CD3 antibody (clone HIT3a, catalog #300319, BioLegend). The 10x library was sequenced on an Illumina HiSeq 2500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... APC-Fire 750 anti-human CD20 (302357, clone 2H7, BioLegend), and APC-H7 anti-human CD3 (560275 ...
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bioRxiv - Immunology 2021Quote: ... and APC-conjugated mouse anti-human IgD (Biolegend, CA, USA). To determine the purity of CB-SC ...