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Citations for BioLegend :
1901 - 1950 of 2095 citations for 6 Quinoxalinamine 2 3 5 trimethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Supernatants were discarded and cells were resuspended in 25 µl of staining solution containing the antibodies detailed in Table 2 diluted in 1X live/dead fixable viability dye (Zombie-Aqua, Biolegend) and incubated 30 min at RT in the dark ...
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bioRxiv - Cancer Biology 2019Quote: ... or 1 million cells (analysis)No in FACS buffer (PBS with 2% FBS, 1X pen/strep and 1 mM EDTA) containing human TruStain FcX (Biolegend) and mouse TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Immunology 2021Quote: ... centrifuged at 200g for 2 minutes followed by ice-cold PBS and incubated with Zombie NIR Fixable Viability dye (Biolegend) to exclude dead cells for 20 minutes at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... Tonsillar IgD+ B cells were isolated from human tonsil cells by positive selection using biotin-anti-human IgD mAb (clone IA6-2; 348212, Biolegend) and MagniSort™ Streptavidin Positive Selection Beads (MSPB-6003-74 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells suspensions of 100 μL were incubated for 15 min at RT with 2 μL Human TruStain FcX (Biolegend, 422301) to prevent nonspecific Fc receptor binding ...
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Molecular Biology 2022Quote: ... or DDX39B-depleted (Sh3 and Sh5) MT-2 cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set (BioLegend). The permeabilized cells were stained with antibodies against FOXP3 conjugated with Alexa Fluor 647 (AB_2539534 ...
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bioRxiv - Immunology 2022Quote: ... The plates were washed twice with PBS plus 2 % FBS and subsequently coated with 10 μg/ml unconjugated anti-CD16 (3GB, BioLegend) in PBS overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... PBMC derived T cells were stimulated for five days at 37°C with 1μg/mL anti-CD3 and anti-CD28 and 10ng/mL of recombinant human IL-2 and recombinant human IL-12p70 (BioLegend). On day four of stimulation ...
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bioRxiv - Immunology 2020Quote: ... Supernatant from eleven donors responding in the ELISPOT assay were profiled using a 12-plex Legendplex T Helper Cytokine Panel Version 2 (Biolegend) following the manufactures instructions ...
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bioRxiv - Immunology 2020Quote: ... at a total concentration of 5×106 cells/mL in RPMI 1640 + 10% FBS + 1% Pen/Strep +50U/mL IL-2 (R10-50) with 1/100 anti-CD107a-PE antibody (Biolegend) and 1/1,000 Monensin GolgiStop™ (BD) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with anti-CoV-2 Spike RBD mAb (SARS2-02) (13) followed by APC-conjugated goat anti-mouse IgG antibody (Cat# 405308, BioLegend). For surface detection ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were activated for 24h using 2 μg/ml of anti-Cd28 and anti-Cd3ε antibodies (102116 and 100340, BioLegend).
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bioRxiv - Immunology 2021Quote: ... The panel for the cohort that received the SARS-CoV-2 spike prime followed by the RBD homotrimer cocktail boost also included SARS-CoV RBD-APC (streptavidin-APC from BioLegend) and WIV1-CoV RBD-BV650 (streptavidin-BV650 from BioLegend) ...
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bioRxiv - Microbiology 2020Quote: ... After incubation the cells were washed twice in FACS buffer (PBS, 0.5% BSA, 2 mM EDTA) and stained with a CD19-APC antibody (BioLegend # 302212) to analyze pDCs for 20 min at 4°C to analyze B cells or with two antibodies directed against CD303(BDCA2)-APC (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained at 4°C for 30 min in Hanks Balanced Salt Solution-2% FBS with the following markers: anti-THY1 AF700 1:100 (Biolegend), anti-PDGFRalpha PE 1:100 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated full-length SARS-CoV-2 Spike protein was purchased from Acro Biosystems and SARS-CoV-2 Spike protein Receptor-Binding Domain (RBD) was purchased from BioLegend.
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bioRxiv - Immunology 2022Quote: The collected BMDCs were centrifuged once and resuspended in cell staining buffer (PBS supplemented with 2% FBS) containing anti-Fc receptor mAb (Biolegend). Cells were blocked for 10 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Sorted 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend), soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Immunology 2020Quote: ... fixed with 2% formaldehyde and permealized with BD Perm/Wash buffer (Fischer) and stained with anti IFN-γ (clone XMG1.2; BioLegend) and anti-GM-CSF (clone MP1-22E9 ...
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bioRxiv - Immunology 2019Quote: ... and stained with CD45.2-FITC (to identify leukocytes) and cell subset specific fluorochrome-conjugated mAb (all were obtained from BD Biosciences and Biolegend). Data were acquired on a BD LSR Fortessa flow cytometer with BD FACS Diva software and analyzed using FlowJo Software version 9.5 (Tree Star ...
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bioRxiv - Physiology 2019Quote: ... Cell culture supernatant was collected and frozen for IL-2 analysis using LEGENDplex™ technology according to the manufacturer’s instructions (Biolegend).
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bioRxiv - Immunology 2019Quote: ... The following antibodies were used for extracellular and intracellular staining as 2 different panels: anti-CD3 Alexa700 (clone UCHT1; BioLegend), anti-CD3 PerCp Cy5.5 (clone UCHT1 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with 2 μg/mL of Alexa Fluor 647 labelled anti-CD5 antibody or anti-CD180 antibody (BioLegend) and 2 μg/mL of Alexa Fluor 488 labelled anti-IgM Fab fragment (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... for 30’ at RT in PBS containing 2% FCS and 1 mM EDTA prior to secondary staining with streptavidin-BrilliantViolet 510 (Biolegend) for 15’ on ice ...
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bioRxiv - Immunology 2020Quote: ... for 8 hours and then surface staining was performed using the following abs anti-human BDCA-2-PE (clone AC144; Biolegend), CD123-PeCy7 (clone 6H6 ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Immunology 2019Quote: ... Blots were blocked 5% BSA in 1x TBS buffer 20mM Tris-HCL/150mM NaCl pH 7.6 for 2 hours and were subsequently incubated with anti-phosphotyrosine secondary antibody (Biolegend; 309302) at 1:1,000 in 5% BSA in TBS ...
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bioRxiv - Immunology 2021Quote: ... The antigenicity and the immunogenicity of the purified recombinant spike protein was validated (Supplementary Fig 1) against verified available recombinant spike protein (BEI Resources, NR52397) and anti-SARS-CoV-2 spike antibody clone A20085C (BioLegend).
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bioRxiv - Immunology 2021Quote: ... IgD-BV510 (clone 11-26c.2a), CD138-BV605 (clone 281-2), CD38-PE-Cy7 (clone 90, CD27-APC (clone LG-3A10) (Biolegend), CD4-FITC (clone GK1.5 ...
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bioRxiv - Immunology 2021Quote: ... sort-purified γδ or αβ IELs were seeded at approximately 400K cells per well in round-bottom 96-well plates coated overnight with 2 μg/ml anti-CD3 (145-2C11, BioLegend), 2 μg/ml polyclonal Armenian hamster IgG (BioXCell) ...
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2021Quote: ... from Miltenyi according to the manufacturer’s instructions and cultured in the above medium supplemented with 2 µg/ml anti-human RP105 antibody clone (312907, BioLegend).
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bioRxiv - Immunology 2021Quote: ... or total combined splenocytes plus bone marrow from WT or c-Rel-/- mice were stimulated for 48 hours in complete RPMI containing 10% fetal bovine serum (FBS) and 1% pen strep with either rmIL-2 (1000 U/ mL; BioLegend), rmIL-15 (100 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 2 mM EDTA in PBS and stained with cell viability dye (Zombie Aqua or Zombie UV, BioLegend). Cells were then incubated with 10 μg/mL anti-CD16/CD32 (2.4G2 ...
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bioRxiv - Cancer Biology 2020Quote: Flow cytometry staining was performed using the antibodies in Supplementary Table 2 in addition to the Zombie Aqua viability stain (BioLegend) and the Foxp3 Transcription Factor Staining Buffer Set (eBioscience) ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Genomics 2022Quote: ... flat bottom 96-well plates were coated with 2 μg/mL of anti-CD3 (Clone: 145-2C11, BioLegend, Cat#100302) in PBS overnight at 4°C or 4 hrs at 37°C ...
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bioRxiv - Immunology 2022Quote: ... 100μg/ml streptomycin and 2 mM EDTA) and stained for 20 minutes at 4 degrees with the following fluorescently conjugated antibodies (BioLegend): anti-CD11c (clone N418) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions were resuspended in PBS with 2% Newborn Calf Serum and stained with TruStain FcX PLUS (S17011E, BioLegend), APC-labeled Ly6G (1A8 ...
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bioRxiv - Immunology 2022Quote: ... washed with FACS buffer (PBS supplemented with 1 mM EDTA and 2% fetal calf serum) and stained with a live/dead marker (viability-eF780, eBiosciences) together with CD19-AF700 (HIB19, BioLegend), CD20-PE-CF594 (2H7 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed 2 times then resuspended in 100 μL wash buffer with 0.1 μg/mL streptavidin-PE (Biolegend 405203) and incubated on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: CAR-NK92 cells and NK92 cells were suspended with fresh culture medium supplied with 2 nM monensin and anti-CD107a-PE antibody (BioLegend) (1:1000 dilution) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...