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Citations for BioLegend :
1851 - 1900 of 7869 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 x with 1% BSA before incubating for 1 h in anti-human IgG secondary antibody labelled with either APC (BioLegend, #410712, dilution 1:100) or Phycoerythrin (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702, 1:250, SATB2, Abcam ab92446 1:250, pS129 α-synuclein (81A, Biolegend 825701, 1:1000).
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Following overnight incubation yeast were washed once in PBS/0.2% BSA and rotated at 18 rpm in same buffer containing 5 µg/mL mouse anti-His6 IgG (BioLegend, San Diego, CA) that had been conjugated with Alexa647 using the N-hydroxysuccinimidyl ester (Molecular Probes ...
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bioRxiv - Bioengineering 2021Quote: ... A 100 µL sample of EDTA-anticoagulated whole blood was incubated with 5 µL PE-Cy5 CD45 (HI30 clone, Biolegend, cat. # 304010) and 5 µL of PE-CD66B (Becton Dickinson ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Harvested lymphocytes were washed three times with FACS buffer (5% FBS in PBS) and stained for surface markers using: CD19-PE (363003, Biolegend, RRID: AB_2564125), IgD-FITC (348206 ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were centrifuged at 300×g for 5 min and washed with 10 mL cell staining buffer (#420201, Biolegend, San Diego, CA). Human FLRT2 antibody (#AF2877 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... IL-4, IL-2, IL-6, IL-10, IL-17A, IL-17F, IL-5, IL-9, IL-13, IL-22; BioLegend Cat# 741044) and Mouse Custom696 Panel (CXCL1(KC) ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Bioengineering 2024Quote: ... Blocking occurred using 5% BSA in TBST and antibody probing using 2% BSA in TBST at the indicated dilutions: α-pERK1/2 (Biolegend 369502) 5000x ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... About 5×106 bone marrow cells were plated into 100mm non-tissue culture treated plates containing 20ng/ml GMCSF (Biolegend, Cat# 640920). On day 3 5ml of fresh media containing 20ng/ml of GMCSF was added to the cultures ...
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bioRxiv - Microbiology 2022Quote: ... the remaining volume was subjected to immunoprecipitation on protein A/G magnetic beads coated with 5 µg of mouse anti-HA IgG (Biolegend, clone 16B12) as described in the Magna RIP technical manual ...
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bioRxiv - Bioengineering 2022Quote: ... 100 µl of 105/ml aAPC-BLMs containing all three ligands were stained with 5 µl of anti-mouse IgG PE (BioLegend, Cat#406607) and anti-human HLA-DR PE (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD4 (RM4-5), (anti-CD90.2 [30-H12], anti-CD44 [IM7], and anti-CD27 [LG-7F9]) (all from eBioscience/Invitrogen and Biolegend, San Diego, CA). Phenotype of polyclonal CD4+ T cell subsets and Plasmodium B5-specific CD4+ T cell subsets were identified with gating strategy as schematically depicted in S7 Fig ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies and channels were used to differentiate leukocyte populations CD4 BV510 (Biolegend Cat. No 100553, Clone RM4-5 0.038ng/µl), CD45 APC (Biolegend Cat ...
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bioRxiv - Immunology 2024Quote: ... in a 5% CO2 incubator at 37 °C for 7 days with 30 ng/ml IL-15 (BioLegend, San Diego, CA, USA) and 10 ng/ml IL-12 (Peprotech ...
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bioRxiv - Biochemistry 2024Quote: ... and cell populations were analyzed by flow cytometry using the following antibodies: Brilliant Violet 510 anti-CD4 (clone RM4-5, BioLegend Cat# 100559), Brilliant Violet 785 anti-CD8a (clone 53-6.7 ...
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bioRxiv - Biochemistry 2024Quote: ... tumor samples from above were analyzed using the following extracellular antibodies: Brilliant Violet 510 anti-CD4 (clone RM4-5, BioLegend Cat# 100559), Brilliant Violet 785 anti-CD8a (clone 53-6.7 ...
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bioRxiv - Cancer Biology 2024Quote: ... RBC lysis was performed by resuspending the PBMCs in 5 ml of 1X RBC lysis buffer (RBC lysis buffer 10X; Biolegend cat# 420301) for 5 mins at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:200 v/v Alexa Fluor 647 anti-mouse/human CD15 (SSEA-1) (Biolegend, 125608) and 1:500 v/v PE anti-mouse/rat CD61 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... β3-tubulin (1:1000, cat. no. 801202, Biolegend; 1:500, cat. no. 302 306, SySy) anti-GFP (IF 1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... rat anti-LAMP1 (DSHB, 1D4B; 1:250) and mouse anti-Neurofilament (BioLegend, 837801; 1:500); and the following secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... CD8-FITC (1:400) or CD8-APC/Cy7 (clone 53-6.7, Biolegend 100714, 1:400) or CD8-BV650 (clone 53-6.7 ...
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bioRxiv - Immunology 2020Quote: ... and PDL-1 PE-Cy7 to visualize the tolerogenic ligand PDL-1 (Biolegend, Catalog #: 124313). After staining for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... anti-F4/80 (clone BM8, 1:100, BioLegend; clone T45-2342, 1:100, BD Biosciences); anti-CD11b (clone M1/70 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice and stained with pSTAT3[Tyr705]-PE (1:10; 13A3-1, BioLegend) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were 1:500 Iba1 (wako 019-19741) and 1:1000 6E10 (BioLegend 803004) diluted in 5% NDS in TBS ...
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bioRxiv - Cancer Biology 2023Quote: ... CD7 (6B7; 1:20) and CD38 (HIT2; 1:20; both from BioLegend, San Diego, CA); CD10 (SJ5-1B4 ...
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bioRxiv - Developmental Biology 2023Quote: ... dilution 1:1000] and Alexa Fluor® 647 CD49f antibody (Biolegend #313610, dilution 1:20) and subsequently segregated into hormone-sensing and alveolar through the staining with Brilliant Violet 605™ Sca-1 antibody (Biolegend #108133 ...
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bioRxiv - Neuroscience 2023Quote: ... containing ssDNA (1 mg/mL final concentration) and FC block (1:100 of BioLegend, 156604), was added ...
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bioRxiv - Cancer Biology 2023Quote: ... CD7 (6B7; 1:20) and CD38 (HIT2; 1:20; both from BioLegend, San Diego, CA); CD10 (SJ5-1B4 ...
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bioRxiv - Immunology 2024Quote: ... cells were first stimulated with cell activation cocktail (1:500 or 1:2500) (Biolegend, 423301), BFA (5 mg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... - αE (clone 2E7, dilution 1:200) (eBioscience), - αLβ2 (clone H155-78, dilution 1:200) (Biolegend), - αM (clone M1/70 ...
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bioRxiv - Immunology 2023Quote: ... and HLA-A3 PE-Vio770 (Miltenyi, 1:100) or HLA-C AF647 (BioLegend, 1:100). Stained cells were washed twice and acquired on a BD LSRII.
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: mouse anti-dynactin 1 (1:250, BioLegend, Cat#867602), mouse anti-dynein (1:1000 ...
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bioRxiv - Immunology 2024Quote: Cultured 1 x 106 THP-1 is immediately fixed by adding Fixation buffer (BioLegend, 420801) pre-warmed to 37 °C ...
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bioRxiv - Immunology 2024Quote: ... CD8-FITC (eBioscience, 11-0083-85, 1:100) or CD4-FITC (Biolegend, 100406, 1:100). CD4+ T cells were recognized as CD45+Ly6-C/G-CD115-TCRβ+CD4+ or CD45+Ly6-C/G-CD115-TCRβ+CD8- ...
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bioRxiv - Immunology 2024Quote: ... 1×106 freshly isolated cells were triple stained with CD11b-Apc (#101211, Biolegend; 1:100), Zombie NIRTM Fixable Viability Kit (#423105 ...
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bioRxiv - Synthetic Biology 2024Quote: ... Alexa Fluor 488 conjugated to anti-β-amyloid 1-16 6E10 (1:200) (Biolegend, 803001) with 488 Conjugation kit (1:200 ...
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bioRxiv - Systems Biology 2023Quote: ... the four-class iPSC dataset (Cell, Mitotic, Dying cell, Debris) was stained with PE anti-human TRA-1-81 (1:300, Biolegend, #330708, clone TRA-1-81), while the five-class iPSC morphology dataset was stained with Alexa Fluor ® 555 anti-GSK3 beta (1:300 ...
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bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant was removed and nanovials were reconstituted at a ten-fold dilution in Washing buffer containing 5 μl of 100 μg/mL anti-IFN-γ BV421 (Biolegend, Catalog: 502532), 25 μg/mL anti-TNF-α APC (Biolegend ...