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Citations for BioLegend :
1801 - 1850 of 1985 citations for P450 Demethylation Activity Kit 2 plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... This pooled sample of ∼300 million cells was then evenly distributed across each of the 372 wells of a LEGENDScreen kit (BioLegend) containing reconstituted PE antibodies (Supplementary Table 2) ...
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bioRxiv - Immunology 2019Quote: ... The presence of human GM-CSF in the supernatant of eMSCs co-cultures with CD34+ HSPCs was assessed by enzyme-linked immunosorbent assay (ELISA) using the human GM-CSF ELISA MAX kit (Biolegend) as per manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Paired maternal and infant plasma were thawed and tested in the 13-plex LegendPlex Human Anti-Virus Response Panel kit (Biolegend), to quantify levels of IL-1β ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... CD3+ T lymphocytes were purified (> 98%) by negative selection with MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, USA). Cells were seeded in CD3-coated plates (clone OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua(tm) Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... FOXP3 APC (Clone FJK-16s eBioscience) used after cell permeabilization with the kit Perm Buffer (10X) and Fix/Perm Buffer (4X) (BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... All other antibodies used for immune fluorescence and flow cytometric analysis and human IL-1beta ELISA kit were acquired from Biolegend. Human was obtained from In vitro experiments were conducted using complete culture media consisting of RPMI 1640 medium supplemented with 20 mM HEPES ...
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bioRxiv - Biochemistry 2022Quote: To monitor the cytotoxicity of MbxA the release of cytosolic LDH was measured as an indicator of membrane damage using the LDH Cytox Assay Kit (Biolegend).
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed for apoptosis/necrosis using the APC Annexin V Apoptosis Detection Kit with Propidium Iodide (BioLegend, San Diego, CA). Cells were analyzed using a BD Symphony Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... The cells were stained extracellularly to detect ILC surface markers and intracellularly for IFNγ expression using the intranuclear staining kit (Biolegend). Sorted ILC1-like and NK cells were stimulated with human (h ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: Levels of IFN-γ in supernatants from T cell clone recognition assays were measured by ELISA using the ELISA MAX Deluxe Set Human IFN-g kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were analyzed by using either the Legendplex Multi-Analyte Flow Assay Kit for human CD8/NK cells or the human Th cytokine panel (Biolegend).
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were either stained with DAPI for viability or fixed with 1% PFA and stained with zombie aqua fixable viability kit (Biolegend). Viable cells were sorted on a FACSAria flow cytometer (Becton Dickinson) ...
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bioRxiv - Microbiology 2020Quote: To assess cell viability human neutrophils at indicated times were washed with PBS and incubated with Zombie Aqua Fixable Viability Kit (BioLegend) for 15 min at RT ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed with PBS and stained with Zombie Aqua fixable live-dead kit as per supplier’s instructions (Biolegend, 423101) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The percentage of apoptotic and necrotic cells were then determined by flow cytometry using the FITC Annexin V apoptosis detection kit with PI (BioLegend) following manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... and either directly fixed for ChIP or stained for dead cells using the Zombi NIR Fixable Viability Kit (Biolegend: #423106) as per the manufacturer’s instructions for subsequent FACS sorting (MHV-68-H2BYFP) ...
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bioRxiv - Cancer Biology 2020Quote: ... washed two times with ice cold PBS and processed for staining using FITC Annexin V Apoptosis Detection Kit with 7AAD (Cat # 640922, BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD4 or CD8 T cell magnetic bead negative selection using the MojoSort Human CD4 or CD8 Selection Kit (BioLegend). Isolated CD4 or CD8 T cells were labeled with CellTrace Violet (CTV ...
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bioRxiv - Microbiology 2021Quote: ... Resting CD4+ T cells were isolated from total PBMCs by negative selection using the MojoSort Human CD4+ T Cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Immunology 2020Quote: ... For human cytokine IL-4 in the supernatant of human ILC2 cell cultures were measured by ELISA kit from BioLegend. All final reactions were developed with TMB substrate (Thermo scientific ...
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bioRxiv - Immunology 2021Quote: ... lymphocytes were stained in 100 μl PBS with a viability dye (Zombie Aqua™ Fixable Viability Kit, Biolegend, the Netherlands) for 15 min at RT in the dark ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Immunology 2022Quote: The presence of IL-17A in T-cell culture supernatants and of IL-6 and IL-8 in fibroblast culture supernatants was quantified by ELISA according to manufacturer instructions (deluxe or standard kits, respectively, all BioLegend). Plates were read at 450 nm using a Spark 10M (Tecan) ...
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bioRxiv - Immunology 2022Quote: The untouched resting B cells were isolated from the human tonsils by depletion of other cells using MojoSort Human B Cell (CD43-) Isolation Kit (BioLegend), whereas the naïve B cells were isolated using the EasySep Human Naïve B cell isolation kit (Stem Cell Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... Intracellular staining for epitopes was achieved using the fixation buffer and intracellular permeabilization buffer kit (BioLegend, San Diego, CA, USA). Antibodies are listed in Table S4 ...
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bioRxiv - Immunology 2022Quote: ... positive selection of the neutrophils was performed by staining the flow through cells with anti-Ly-6G-APC for 20 minutes at 40C followed by isolating the stained cells with anti-APC nanobeads using a mojosort APC nanobead kit (Biolegend). Purity and viability of the cells were checked by flow cytometry using an anti-mouse Gr1 antibody and fixable viability dye ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
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bioRxiv - Cancer Biology 2023Quote: ... Comprehensive surface immunophenotyping was performed using LEGENDScreen Human PE Kit containing 371 pre-titrated antibodies and 354 unique specificities (BioLegend). The experimental run was multiplexed using GFP+ NCI-H358 cells resistant to sotorasib combined with non-fluorescent ...
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bioRxiv - Immunology 2022Quote: ... plasma and tumor lysates were performed by flow cytometry using a multiplex bead-based system (LEGENDPlex Mouse Th Cytokine Panel and Mouse Inflammation Panel kits, Biolegend) that allowed the simultaneous determination of the concentration of several cytokines ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
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bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were collected from effector-target cell co-cultures and analyzed for IFNy using either ELISA MAX Deluxe Human IFNγ kit (BioLegend) or Human IFNγ DuoSet ELISA (R&D Systems ...