Glutathione Reductase Fluorescent Kit 1 Plate citations for BioLegend

Quote: ... Sections were incubated with different primary antibodies (Rat-anti-MBP, Sigma-Aldrich MAB386, 1:1000, monoclonal; Rabbit-anti-GFAP, CiteAb Z0334, 1:1000, polyclonal; Mouse-anti-6E10 Amyloid-β, BioLegend SIG-39300, 1:1000, monoclonal) diluted in 200 μl 10% NDS and 0.4% Triton-X blocking medium ON at 4°C ...

Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium

Raphael Brandt, et al., bioRxiv - Cell Biology 2019

Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...

Microenvironment-contextual cell signaling is attenuated with age

Henriette C. Ertsås, et al., bioRxiv - Cell Biology 2019

Quote: ... from BD biosciences and APC-Cy7 anti-CD10 (1/100, #312212) PE anti-CD117 (1/100, #313210) from Biolegend.

Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer

Florian Uhlitz, et al., bioRxiv - Cancer Biology 2021

Quote: ... organoids were harvested in PBS and digested to a single cell solution in 1:1 Accutase (Biolegend) and TrypLE (Gibco ...

Protection from viral rebound after therapeutic vaccination with an adjuvanted DNA vaccine is associated with SIV-specific polyfunctional CD8 T cells in the blood and mesenteric lymph nodes

Hillary C. Tunggal, et al., bioRxiv - Microbiology 2020

Quote: ... or SIV Gag or Env peptides (1 μg/mL) for 1 hour with CD107a PECy5 (eBioH4A3, BioLegend) in R10 media before adding 1 mg/mL of Brefeldin A (Sigma-Aldrich®) ...

Human lung organoids develop into adult airway-like structures directed by physico-chemical biomaterial properties

Briana R Dye, et al., bioRxiv - Bioengineering 2019

Quote: ... and Pacific Blue™ anti-Ly-6G/Ly-6C (Gr-1) (1:70, clone RB6-8C5, Biolegend).

Disruption of the Blood-Brain Barrier in 22q11.2 Deletion Syndrome

Alexis M. Crockett, et al., bioRxiv - Neuroscience 2019

Quote: ... and cells were stained with rat anti-mouse ICAM-1 (1:100; Biolegend YN1/1.7.4 in PerCP), rat anti-mouse CD45 (1:100 ...

Improved metabolic syndrome and timing of weight loss is crucial for influenza vaccine-induced immunity in obese mice

Rebekah Honce, et al., bioRxiv - Microbiology 2022

Quote: ... 1×106 cells were plated and then incubated with Fc block (Biolegend; cat#101320; 1:100 dilution) and surface stained for 20 min at 4°C in the dark ...

Spermidine suppresses DC activation via eIF5A hypusination and metabolic adaptation

Gavin R Meehan, et al., bioRxiv - Immunology 2023

Quote: ... CD40-APC (1:100) and CD11c-e450 (1:100) (all antibodies from Biolegend, San Diego, CA, USA). Cells were incubated with 100μl of the antibody suspension for 20 minutes at 4°C before a final wash in Ca2+/Mg2+ free PBS containing 2mM EDTA at x400g for 5 minutes at 4°C ...

Antibody targeting of surface PSGL-1 glycoprotein leads to lymphoma apoptosis and tumorigenesis inhibition

João L. Pereira, et al., bioRxiv - Cancer Biology 2024

Quote: ... approximately 5-10 µg/ml) or purified KPL-1 anti-PSGL-1 mAb (cat. no. 328802, Biolegend). The PL1 hybridoma developed by McEver ...

Interpretable deep learning reveals the sequence rules of Hippo signaling

Khyati Dalal, et al., bioRxiv - Genomics 2024

Quote: ... at a 1:300 dilution with DAPI 2 μl in 1 ml of 10 μg/ml (BioLegend) and kept on light rotation for 2 h at RT ...

A panel of phenotypically and genotypically diverse bioluminescent:fluorescent Trypanosoma cruzi strains as a resource for Chagas disease research

Francisco Olmo, et al., bioRxiv - Microbiology 2024

Quote: ... and neurons were labelled with rabbit anti-tubulin beta 3 (TuJ-1) IgG at 1:500 (Biolegend), in PBS ...

HDAC1-3 inhibition reduces CCR2 expression and immunosuppressive function of myeloid-derived suppressor cells

Zhiqi Xie, et al., bioRxiv - Immunology 2023

Quote: ... T cell proliferation was stimulated with anti-CD3ε (1 μg/mL)/CD28 (1 μg/mL) antibodies (BioLegend). The anti-CD3ε antibody was coated on each well of the plate via overnight incubation at 4°C with PBS before coculture ...

Defining the mechanisms of action and resistance to the anti-PD-1+LAG-3 and anti-PD-1+CTLA-4 combinations in melanoma flank and brain models

Manali S. Phadke, et al., bioRxiv - Immunology 2023

Quote: ... 1 × 106 cells were blocked with purified mouse CD16/32 antibody (1:100 dilution; cat. #101301; BioLegend) for 5 minutes on ice ...

Generation of a human Tropomyosin 1 knockout iPSC line

Madison B Wilken, et al., bioRxiv - Cell Biology 2023

Quote: ... Alexa-Fluor®-488 SSEA1 (CD15) (1:50) and Alexa-Fluor®-647 SSEA5 (1:200) (BioLegend). In all experiments ...

SR-BI regulates the synergistic mast cell response by modulating the plasma membrane-associated cholesterol pool

Sandro Capellmann, et al., bioRxiv - Immunology 2023

Quote: ... ST2 and CD13 was verified using FITC-coupled anti-FcεRI (1:100, clone MAR-1, BioLegend, #134306), PE-coupled anti-KIT (1:100 ...

Sodium chloride in the tumor microenvironment enhances T-cell metabolic fitness and cytotoxicity

Dominik Soll, et al., bioRxiv - Immunology 2023

Quote: ... rhIL-2 (50 Uml-1, Novartis) and anti-mIFN-γ (5 µg ml-1, clone XMG1.2, Biolegend) in the presence or absence of 30 mM NaCl ...

BBB recovery after stroke by self-replenishing E-pericytes transdifferentiated from local endothelial cells

Li Tingbo, et al., bioRxiv - Neuroscience 2024

Quote: ... The following antibodies in 1:200 dilutions were used: CD31-PE-594 (1:200, Biolegend, Cat# 102520), CD45-APC (1:200 ...

Perinatal brain group 3 innate lymphoid cells are involved in the formation of murine dural lymphatics

Alba del Rio Serrato, et al., bioRxiv - Immunology 2024

Quote: ... Cells were stained with BV711 (1:50) or AlexaFluor647 (1:300) anti-mouse T-bet (Biolegend, 4B10), PE eFluor610 anti-mouse Eomes (Biolegend ...

Cytosolic S100A8/A9 promotes Ca²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

Matteo Napoli, et al., bioRxiv - Physiology 2024

Quote: ... and immunofluorescence staining for PECAM-1 (AlexaFluor488 labelled primary monoclonal rat antibody, 5μg mL-1, MEC13.3, BioLegend) and S100A9 (Cy5.5 directly labelled ...

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