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Citations for BioLegend :
1701 - 1750 of 6274 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cells were stained with anti-CD4 BV605 (#100548, clone RM4-5, 1:700, Biolegend), anti-CD3 BV711 (#100241 ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend, 344716) per 6 μL nanovial volume ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor tissue was chopped finely with a razor in 5 ml Accutase (Biolegend, #4232201) and incubated at 37°C water bath for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and DRAQ5 (1ng/mL, incubation 5 minutes at RT; BioLegend, San Diego, CA, USA). Samples were measured using a FACS Canto II (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... XCR1 (clone ZET) and CD64 (FcγRI) (clone X54-5/7.1) were obtained from BioLegend.
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cells were stained with PE-coupled anti-Acetylated Lysine Antibody or PE-coupled Mouse IgG2b κ Isotype control Antibody (isotype control) (both Biolegend) for 30 min at 4°C ...
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bioRxiv - Genomics 2022Quote: ... we stained cells with CITE-seq and hashing antibodies as previously described 22 (Supplementary Table 4) along with a fluorescent HLA antibody (BioLegend 311415). The 5% lowest expressing HLA cells were selected via FACS and 40,000 cells were loaded onto one 10x 3’ V3 Chromium channel ...
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bioRxiv - Genetics 2020Quote: Immunoprecipitation and immunoblotting were carried out according to standard protocols with the following antibodies: rat anti-Flag L5 antibody (BioLegend; 637301), rabbit anti-GFP antibody (Novus ...
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bioRxiv - Immunology 2022Quote: ... FcBlock was added for 10 min prior to fluorescent antibody staining for surface proteins for 30 min at 4°C using the following antibodies: BV510 anti-mouse CD45 (Biolegend 103137), FITC anti-mouse CD11b (eBioscience 11-0112-86) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4 °C with primary antibody (Biotin anti-β-Amyloid, 1-16 Antibody, clone 6E10 from BioLegend) dissolved in a carrier solution made with 1% HS ...
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bioRxiv - Genetics 2022Quote: ... Differentiation efficiency was measured using Fluorescence Activated Cell Sorting (FACS) following staining with an APC conjugated antibody to HLAG (APC anti-human HLA-G Antibody Clone: 87G, BioLegend UK), in unmodified hTSC this was consistently between 40-70% ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with fluorescent antibodies for intracellular proteins for 30 min at 4°C using the following antibodies: PE anti-mouse TNF (Biolegend 506306) and APC anti-mouse IL-6 (Biolegend 503810) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The blocking buffer was subsequently removed and cells were incubated for an additional hour with 1:100 diluted primary antibody (αHA antibody, Biolegend, #901502) in antibody dilution buffer (1x PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Western blotting was performed using an anti-HA mouse monoclonal antibody (Alexa Fluor® 488 anti-HA.11 Epitope Tag Antibody, BioLegend) as primary antibody diluted 1:1000 in TBS-T (20 mM Tris ...
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bioRxiv - Genetics 2024Quote: ... Another 400 μL of supernatant was mixed with 600 μL of FSPP buffer and 20 μL of pre-blocked Magnetic IgG beads and TAP antibody (Thermo CAB1001) or Rpb3 antibody (Biolegend 920204) for 10–12 hrs at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Neuroscience 2023Quote: ... non-fat dried skimmed milk powder and then probed with ICSM35 anti-PrP antibody (D-Gen Ltd; 1:5000 for RML) or with 6D11 anti-PrP antibody (BioLegend (808004); 1:5000 for ME7 ...
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bioRxiv - Bioengineering 2023Quote: Streptavidin-coated nanovials were reconstituted at a five time dilution in Washing Buffer containing 140 nM (20 μg/mL) of each biotinylated antibody or cocktail of antibodies: anti-CD45 (Biolegend, 368534) and anti-IFN-γ (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for detection of MDSCs contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106), a rat anti-mouse Ly-6G/Ly-6C (Gr1 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions) were incubated with 10 nM of a second CD98hc antibody for detection (RL388, rat anti-mouse antibody; BioLegend, 128202). Non-treated cells were also incubated with the TfR-1 (R17 ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... brains were incubated with 1:1000 chicken anti-GFP antibody (Aves GFP-1020) and 1:1000 mouse anti-tyrosine hydroxylase antibody (BioLegend 818001), with boosters over 5 days for a final concentration of 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... separate tubes were designated for FITC anti-human CD80 Antibody and APC/Cyanine7 anti-human CD206 (MMR) Antibody (BioLegend Europe B.V.) staining with an incubation time of 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were treated with CD16/CD32 blocking antibody (Ultra-LEAF Purified Anti-mouse CD16/32 Antibody, Biolegend, San Diego, CA, USA), to prevent phagocytosis ...
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Neuroscience 2021Quote: ... The following antibodies were used to immunotag nuclei: anti-H2A.X-Phosphorylated (Ser139) antibody conjugated to APC (cat no. 613416, BioLegend, San Diego CA), anti-ATM-phospho (Ser1981) ...
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bioRxiv - Cell Biology 2019Quote: ... Mouse anti-β-III-tubulin antibody TUJ1 (801202) and mouse anti-neurofilament monoclonal antibody SMI312 (837904) were from Biolegend (San Diego, CA). Mouse anti-α-tubulin antibody (DM1A ...
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: The following antibodies were used for flow cytometry: Brilliant Violet 510™ anti-mouse CD45 Antibody (BioLegend, catalog #103137, clone 30-F11), PE/Dazzle™ 594 anti-mouse CD3ε Antibody (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... cell hashing was performed using TotalSeq-A antibodies (BioLegend) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-TNF neutralizing antibody (#506325) was obtained from Biolegend.
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bioRxiv - Cell Biology 2020Quote: ... surface-stained using antibodies raised against cKIT (2B8; Biolegend) and SCA1 (D7 ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (BV421-conjugated donkey anti-rabbit (406410, Biolegend) were added in brilliant stain buffer (BD ...
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bioRxiv - Cell Biology 2020Quote: Mouse anti-beta-actin antibody (clone 2F1-1, Biolegend)
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bioRxiv - Immunology 2021Quote: ... the following antibodies were used (all purchased from BioLegend): anti- CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... The antibody cocktail included FITC-CD45 (BioLegend Cat# 103108), BV421-CD44 (BioLegend Cat# 103040) ...
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bioRxiv - Immunology 2021Quote: ... with either 10 μg anti-DR3 antibody (4C12, Biolegend) or 10 μg Armenian Hamster IgG control antibody (HTK888 ...
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bioRxiv - Immunology 2021Quote: ... and incubated with an anti-rabbit secondary antibody (Biolegend) if needed ...
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bioRxiv - Molecular Biology 2021Quote: ... and PE-conjugated anti-mouse IL1a antibody (clone:ALF161, BioLegend) containing 500 μl FACS buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated with Ki67-FITC antibody (Biolegend, 652410) for 30 min at room temperature followed by DAPI staining ...
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bioRxiv - Immunology 2022Quote: ... All the flow cytometry antibodies were obtained from BioLegend, and Flow cytometry was performed using BD FACSAria III sorter (BD biosciences ...
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bioRxiv - Immunology 2022Quote: ... Engraftment was measured using the following antibodies from Biolegend: anti-CD45.1 (APC- Cy7 ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were purchased from BioLegend (San Diego, CA, USA). Cell fluorescence was measured using an LSR Fortessa (BD Biosciences ...