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1701 - 1750 of 3014 citations for Recombinant Human ENG Protein since 2019

• Relationship between therapeutic activity and preferential targeting of toxic soluble aggregates by amyloid-beta-directed antibodies

  Johanne M. Kaplan, et al., bioRxiv - Neuroscience 2024

  Quote: ... Human IgG1 isotype control was purchased from BioLegend (San Diego, CA, USA).
• Label-Free Electrochemical Imaging of Single Extracellular Vesicles

  Yuanxin Zhang, et al., bioRxiv - Biophysics 2024

  Quote: ... Alexa Fluor 647 anti-human CD63 antibody (353016) was obtained from BioLegend. Alexa Fluor 488-labeled mouse anti-human CD81 antibody (MCA1847A488 ...
• Coxsackievirus group B3 regulates ASS1-mediated metabolic reprogramming and promotes macrophage inflammatory polarization in viral myocarditis

  Qiong Liu, et al., bioRxiv - Microbiology 2024

  Quote: ... and PE anti-mouse/human CD11b antibody were purchased from BioLegend (USA); anti-CD86 (rabbit) ...
• Follicular helper- and peripheral helper-like T cells drive autoimmune disease in human immune system mice

  Andrea Vecchione, et al., bioRxiv - Immunology 2024

  Quote: ... Then anti-human CD3 Alexa Fluor 488 (UCHT1, Biolegend, 1:50 dilution), anti-human CD20 Alexa 647 (Abcam ...
• Perinatal brain group 3 innate lymphoid cells are involved in the formation of murine dural lymphatics

  Alba del Rio Serrato, et al., bioRxiv - Immunology 2024

  Quote: ... APC-Cy7 anti mouse/human CD45R/B220 (Biolegend, RA3-6B2, 1:100), APC-eFluor 780 anti-mouse CD45R/B220 (eBioscience ...
• Synergically enhanced anti-tumor immunity of in vivo CAR by circRNA vaccine boosting

  Yanyan Wang, et al., bioRxiv - Immunology 2024

  Quote: ... and subsequently stained with PE/Cyanine7 anti-mouse/human CD11b (BioLegend, #101216), PE anti-iNOS Antibody (BioLegend ...
• CD28 Shapes T Cell Receptor Signaling by Regulating ZAP70 Activation and Lck Dynamics

  Kumarkrishna Raychaudhuri, et al., bioRxiv - Immunology 2024

  Quote: ... Human anti-CD43 (Clone: S11) was purchased from BioLegend (Cat. No.143201) and was used to coat glass bottom chambers for imaging studies ...
• Nasal tissue-resident memory CD4⁺ T cells persist after influenza A virus infection and provide heterosubtypic protection

  Nimitha R. Mathew, et al., bioRxiv - Immunology 2024

  Quote: PBMC and NT cells were incubated with Human Trustain FcX (Biolegend, 422302) for 10 min at 4°C ...
• Chlamydia-driven ISG15 expression dampens the immune response of epithelial cells independently of ISGylation

  Yongzheng Wu, et al., bioRxiv - Microbiology 2024

  Quote: ... or 1/80 dilution of PE-conjugated anti-human IL8 (Biolegend, #511408) for 1h ...
• Intracellular Cryptococcus neoformans disrupts the transcriptome profile of M1- and M2-polarized host macrophages

  Aarthi Subramani, et al., bioRxiv - Immunology 2020

  Quote: ... RAW 264.7 cells were seeded into 6-well plates at a density of 7.5 x 105 cells/well and incubated overnight with 200 U/mL of recombinant murine IFNγ (Biolegend, San Diego, CA) to promote M1-polarization and then infected with opsonized Cn for 2 h at 3:1 MOI or mock infected with PBS ...
• Cancer cell genetics shaping of the tumor microenvironment reveals myeloid cell-centric exploitable vulnerabilities in hepatocellular carcinoma

  Christel FA Ramirez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... M- CSF and GM-CSF differentiated BM cells were cultured with complete medium +10ng/ml recombinant mouse M-CSF (Biolegend, cat.no. 576408) or +20ng/ml recombinant GM-CSF (Peprotech ...
• Murine uterine gland branching is necessary for gland function in implantation

  Katrina Granger, et al., bioRxiv - Developmental Biology 2023

  Quote: ... XER mice were injected intraperitoneally at 1000h and 1800h on GD3 with either PBS or 10µg recombinant Lif (554006, BioLegend, San Diego, CA) followed by blue dye injection and dissection at GD4 1200h ...
• Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

  Katrin Fischer, et al., bioRxiv - Immunology 2023

  Quote: Biotinylated S1 protein was obtained commercially (BioLegend). A 4X molar excess was incubated with streptavidin-Alexa Fluor 647 (0.5 mg/mL ...
• Human alveolar Type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells during alveolar repair

  Jaymin J. Kathiriya, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-human CD31 APC-Cy7 (BioLegend; Cat#303120; used at 1:200), mouse anti-human CD326 PE (BioLegend ...
• Nanoengineered DNA origami with repurposed TOP1 inhibitors targeting myeloid cells for the mitigation of neuroinflammation

  Keying Zhu, et al., bioRxiv - Immunology 2021

  Quote: ... PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend, 101228, clone M1/70, 1:100), APC anti-mouse F4/80 (BioLegend ...
• The vaccinia-based Sementis Copenhagen Vector COVID-19 vaccine induces broad and durable cellular and humoral immune responses

  Preethi Eldi, et al., bioRxiv - Immunology 2021

  Quote: ... and anti-human/mouse granzyme B PE-Cy7 (clone Q1A6A02, 1:200, BioLegend). All antibodies were diluted in Brilliant stain buffer (BD Biosciences ...
• Exploring the rules of chimeric antigen receptor phenotypic output using combinatorial signaling motif libraries and machine learning

  K.G. Daniels, et al., bioRxiv - Synthetic Biology 2022

  Quote: ... Antibodies are as follows: APC Mouse anti-human KLRG1 clone SA231A2 (BioLegend #367716), BV421 Mouse anti-human IL7Rα clone HIL-7R-M21 (BD Biosciences #562436) ...
• Effect of stimulation time on the expression of human macrophage polarization markers

  Duygu Unuvar Purcu, et al., bioRxiv - Immunology 2022

  Quote: ... Fcγ receptors were blocked with Human TruStain FcX antibody (BioLegend, San Diego, CA) for 10 min and surface antigens on cells were stained for 40 min at 4 °C ...
• CD155 and EndoA1 mediate growth and tissue invasion downstream of MAP4K4 in medulloblastoma cells

  Charles Capdeville, et al., bioRxiv - Cell Biology 2022

  Quote: ... cells were stained with PE-labelled anti-human CD155 (BioLegend, 337610, 1:300), corresponding isotype control antibody (BioLegend ...
• ART duration and immunometabolic state determine efficacy of DC-based treatment restoring functional HIV- specific CD8+ T cells in PLWH

  Marta Calvet-Mirabent, et al., bioRxiv - Immunology 2022

  Quote: ... memory cells were purified after treatment with mouse anti-human CD45RA mAbs (Biolegend) and Goat anti-Mouse IgG covered Dynabeads (Invitrogen).
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD29 PE Cy7 (1:100, clone TS2/16, BioLegend, cat # 303025), Anti-human CD45RO PE Cy7 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CRTh2 PE Dazzle 594 (1:50, clone BM16, BioLegend, cat # 350126), Anti-human CD31 PE Dazzle 594 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD31 PE Dazzle 594 (1:100, clone WM59, BioLegend, cat # 303130), Anti-human CD16 PE Dazzle 594 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD16 PE Dazzle 594 (1:100, clone 3G8, BioLegend, cat # 302054), Anti-human CD45 PE-CF594 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD49d PE Dazzle 594 (1:100, clone 9F10, BioLegend, cat # 304325), Anti-human CRTh2 PE Dazzle 594 (1:50 ...
• Beyond Spike: Identification of nine highly prevalent SARS-CoV-2-specific CD8 T-cell epitopes in a large Norwegian cohort

  Saskia Meyer, et al., bioRxiv - Immunology 2021

  Quote: ... BV785 anti-human CD56 (clone 5.1H11; dilution 1:100; Biolegend; Cat no 362550); BV785 anti-human CD14 (clone M5E2 ...
• Beyond Spike: Identification of nine highly prevalent SARS-CoV-2-specific CD8 T-cell epitopes in a large Norwegian cohort

  Saskia Meyer, et al., bioRxiv - Immunology 2021

  Quote: ... BV785 anti-human CD38 (clone HIT2; dilution 1:100; Biolegend; Cat no Biolegend); BV785 anti-human CD19 (clone HIB19 ...
• Beyond Spike: Identification of nine highly prevalent SARS-CoV-2-specific CD8 T-cell epitopes in a large Norwegian cohort

  Saskia Meyer, et al., bioRxiv - Immunology 2021

  Quote: ... PE anti-human CD134 (clone OX40; dilution 1:100; Biolegend; Cat no 350004), BV421 anti-human CD69 (clone FN50 ...
• Beyond Spike: Identification of nine highly prevalent SARS-CoV-2-specific CD8 T-cell epitopes in a large Norwegian cohort

  Saskia Meyer, et al., bioRxiv - Immunology 2021

  Quote: ... BV785 anti-human CD19 (clone HIB19; dilution 1:100; Biolegend; Cat no 302240); BV785 anti-human CD56 (clone 5.1H11 ...
• Beyond Spike: Identification of nine highly prevalent SARS-CoV-2-specific CD8 T-cell epitopes in a large Norwegian cohort

  Saskia Meyer, et al., bioRxiv - Immunology 2021

  Quote: ... BV785 anti-human CD14 (clone M5E2; dilution 1:100; Biolegend; Cat no 301840); BV785 anti-human CD4 (clone RPA-T4 ...
• Beyond Spike: Identification of nine highly prevalent SARS-CoV-2-specific CD8 T-cell epitopes in a large Norwegian cohort

  Saskia Meyer, et al., bioRxiv - Immunology 2021

  Quote: ... BV785 anti-human CD38 (clone HIT2; dilution 1:100; Biolegend; Cat no Biolegend); BV785 anti-human CD19 (clone HIB19 ...
• Engineered niches support the development of human dendritic cells in humanized mice

  Giorgio Anselmi, et al., bioRxiv - Immunology 2019

  Quote: ... plugs sections were labeled overnight with purified mouse anti-human CD45 (HI30, Biolegend) followed by 1hr incubation at room temperature with goat anti-mouse Cy3 ...
• A Multi-Niche Microvascularized Human Bone-Marrow-on-a-Chip

  Michael R. Nelson, et al., bioRxiv - Bioengineering 2019

  Quote: ... HSPCs were then stained overnight using anti-human CD45 Alexa Fluor 488 (BioLegend). Samples were imaged using a spinning disk confocal microscope (PerkinElmer ...
• An atlas of infiltrated B-lymphocytes in breast cancer revealed by paired single-cell RNA-sequencing and antigen receptor profiling

  Qingtao Hu, et al., bioRxiv - Immunology 2019

  Quote: Antibodies used in this study included PE anti-human CD45 (BioLegend, Cat#304008), PerCP-CyTM5.5 Mouse Anti-Human CD56 (BD ...
• TREM-1 activation is a key regulator in driving severe pathogenesis of enterovirus 71 infection

  Siti Naqiah Amrun, et al., bioRxiv - Immunology 2019

  Quote: ... Cell surface staining was performed with mouse anti-human CD45 (clone HI30; BioLegend), CD3 (clone SP34-2 ...
• Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics

  Christopher H. Hulton, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Approximately 1 million cells were resuspended in PBS containing human TruStain FcX (Biolegend) and incubated at 4C for 10 minutes ...
• Direct genome editing of patient-derived xenografts using CRISPR-Cas9 enables rapid in vivo functional genomics

  Christopher H. Hulton, et al., bioRxiv - Cancer Biology 2019

  Quote: ... Cells were stained with APC/Cy7 anti-human CD4 (clone RPA-T4, Biolegend) and AlexaFluor647 anti-mouse H-2Kd (clone SF1-1.1 ...
• Metformin abrogates pathological TNF-α-producing B cells through mTOR-dependent metabolic reprogramming in polycystic ovary syndrome

  Na Xiao, et al., bioRxiv - Cell Biology 2021

  Quote: Cell surface staining was performed with human CD19-PE (clone HIB 19; BioLegend) antibodies ...
• Surface Engineering of FLT4-Targeted Nanocarriers Enhances Cell-Softening Glaucoma Therapy

  Michael P. Vincent, et al., bioRxiv - Bioengineering 2021

  Quote: ... cells were incubated with an APC-conjugated anti-human VEGFR3 (FLT4) antibody (BioLegend) for 20 minutes at 4 °C and were then subjected to two iterative rounds of washing (and centrifugation ...
• A human disease model of SARS-CoV-2-induced lung injury and immune responses with a microengineered organ chip

  Min Zhang, et al., bioRxiv - Bioengineering 2020

  Quote: ... and TNF-α were measured using the corresponding human ELISA kits (Biolegend, USA) according to the manufacturer’s instructions.
• An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants

  Kui K. Chan, et al., bioRxiv - Biochemistry 2020

  Quote: ... and stained with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
• Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants

  Yi-Nan Zhang, et al., bioRxiv - Immunology 2021

  Quote: ... Brilliant Violet 510 anti-mouse/human CD45R/B220 antibody (BioLegend, catalog no. 103247), FITC anti-mouse CD3 antibody (BioLegend ...
• Postnatal lymph node expansion of stromal progenitors towards reticular and CD34⁺ stromal cell subsets is determined by distinct transcriptional programs

  Joern Pezoldt, et al., bioRxiv - Immunology 2021

  Quote: Fluorochrom-conjugated anti-human anti-CD24 (clone M1/69, APC, BioLegend Cat. #101814), anti-CD31 (clone 390 ...
• FACT subunit SUPT16H associates with BRD4 and contributes to silencing of antiviral interferon signaling

  Dawei Zhou, et al., bioRxiv - Molecular Biology 2021

  Quote: ... PE anti-human IFNγ and anti-IL-6 antibodies were purchased from BioLegend. Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich ...
• A Novel CRISPR-Engineered, Stem Cell-Derived Cellular Vaccine

  Krishnendu Chakraborty, et al., bioRxiv - Immunology 2021

  Quote: ... and were then stained with PE anti-human MICA/MICB Antibody (6D4, Biolegend), Alexa Fluor 647 anti-human HLA-A,B,C (W6/32 ...
• SF3B1 mutant-induced missplicing of MAP3K7 causes anemia in myelodysplastic syndromes

  Yen K. Lieu, et al., bioRxiv - Cancer Biology 2020

  Quote: ... FACS antibodies used were the following: anti-human CD235a (GYPA) PE (Biolegend, 349106), anti-AnnexinV APC (Biolegend ...
• A versatile, chemically-controlled DNA binding switch enables temporal modulation of Cas9-based effectors

  Cindy T. Wei, et al., bioRxiv - Molecular Biology 2022

  Quote: ... cells were harvested and incubated with APC anti-human CD184 (CXCR4) [12G5] (BioLegend) for 1 hr and then fluorescence was analyzed on the LSRII flow cytometer (BD Biosciences) ...
• Omicron BA.2 specifically evades broad sarbecovirus neutralizing antibodies

  Yunlong Cao, et al., bioRxiv - Immunology 2022

  Quote: ... B cells were then stained with FITC anti-human CD19 antibody (BioLegend, 392508), FITC anti-human CD20 antibody (BioLegend ...
• Siglec-5 is an inhibitory immune checkpoint molecule for human T cells

  Aleksandra Vuchkovska, et al., bioRxiv - Immunology 2022

  Quote: ... pre-incubated with anti-human IgG Fc fluorescent antibody (Biolegend, San Diego, CA). As a negative control ...
• Siglec-5 is an inhibitory immune checkpoint molecule for human T cells

  Aleksandra Vuchkovska, et al., bioRxiv - Immunology 2022

  Quote: ... and IL-22 using the LEGENDplex Human Th Cytokine (Biolegend, San Diego, CA). Cells from 3-day long re-stimulated cultures were used for evaluating expression of ...