Labshake search
Citations for BioLegend :
1701 - 1750 of 2034 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Slides were incubated with antibody cocktails in blocking buffer overnight at 4°C in a moisture chamber: Amyloid-β (6E10) (1:100; Biolegend, 803001), anti-CHMP2B (1:100 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were incubated with antibody cocktail for surface markers at 4 °C for 30 min and then fixed using True nuclear transcription factor buffer set (Biolegend, Cat # 424401) for 20 min at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... PBMCs were stimulated as a single-cell suspension at a concentration of 4-5 x 106 cells/mL with the Cell Activation Cocktail (Biolegend, Cat # 423301) and incubated at 37°C with 5% CO2 ...
-
bioRxiv - Biochemistry 2024Quote: ... in RPMI complete medium in a 6-well plate that was pre-coated overnight at 4°C with anti-CD3 antibody (BioLegend Cat# 100340) at 100 pg/mL ...
-
bioRxiv - Biochemistry 2024Quote: ... 96-well high-binding plates were coated overnight at 4°C with 21h10 protein (500 ng per well) diluted in 1X coating buffer (from ELISA set, Biolegend Cat#430804), and blocked with PBS + 10% IFS for 24 hours at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... for 40 minutes at 4 °C followed by a secondary staining with anti-mouse IgG (1:200, Poly4053-PE- Cy7, Biolegend, Cat # 405315) or anti-mouse IgM (1:200 ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were fixed with 4% PFA in PBS and exposed to 1:1000 anti-HA mouse monoclonal antibody (BioLegend Cat#901514, RRID:AB_2565336) or 1:1000 anti-FLAG mouse monoclonal antibody (M2 ...
-
bioRxiv - Cell Biology 2024Quote: ... the samples were incubated in 3% BSA for 1 hr before being treated overnight at 4°C with the respective primary antibodies : PAX6 for ectoderm (1:300; BioLegend, PRB-278P), Brachyury for mesoderm (1:300 ...
-
bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...
-
bioRxiv - Cell Biology 2020Quote: ... Supernatant was discarded and the pellet was resuspended in 100µl FACS Stain Buffer (2% FBS in PBS) with 1µl anti-CD45-APC/Cy7 (clone 30-F11, BioLegend) and 1µl anti-EPCAM-AlexaFluor488 (clone G8.8 ...
-
bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Digested meniscus cells were re-suspended in flow buffer (2% FBS/PBS) and stained with Sca1 (BioLegend, 108131), Cd90 (BioLegend ...
-
bioRxiv - Neuroscience 2021Quote: ... Brilliant Violet 421 or Alexa Fluor 594 conjugated anti-mouse CD8a (2 μg/mL; clone 53-6.7; BioLegend) was used ...
-
bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
-
bioRxiv - Molecular Biology 2021Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: ... Cell hashing antibodies targeting human Beta-2-microglobulin (clone 2M2) and CD298 (clone REA217) were purchased from Biolegend and Miltenyi Biosciences ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-Vimentin (Santa-Cruz; 2 μg/mL) and mouse anti-placental alkaline phosphatase (Biolegend; 1 μg/mL). Immunostaining for hCMV was performed as previously described [32] ...
-
bioRxiv - Immunology 2022Quote: ... with PE-conjugated anti-mouse H-2 or I-A/I-E Ab and corresponding isotype controls (BioLegend) (for the MHC study) ...
-
bioRxiv - Immunology 2022Quote: Whole blood inoculated with SARS-CoV-2 was blocked with 5μl of TruStain (Biolegend, San Diego, California, # 422302) for 10 minutes at room temperature and then stained for granulocyte and monocyte markers ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Neuroscience 2022Quote: ... Dead cells were excluded by labelling with the cell viability dye Zombie Aqua (2 ng/ml, BioLegend, 423101) that was added to the single cell suspension before sorting ...
-
bioRxiv - Molecular Biology 2020Quote: ... TCRA ΔPAR or TCRB ΔPAR cells were measured by ELISA MAX™ Deluxe Set Human IL-2 (BioLegend) according to the manufacturer’s instructions.
-
bioRxiv - Physiology 2023Quote: ... IL-6 and FFA content were measured by ELISA (IL-1β, Biolegend, 432604; IL-6, Invitrogen, BMS603-2) or a FFA fluorometric kit (Cayman Chemical ...
-
bioRxiv - Bioengineering 2023Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...
-
bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
-
bioRxiv - Immunology 2024Quote: ... Mouse and human T cells were washed three times with RPMI 10% FBS and 2.5 x 105 cells were added to each well with 2 µg/ml soluble anti-CD28 (37.51 or CD28.2, Biolegend). For co-culture experiments ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 2% PFA for 20min and permeabilized with Perm/Wash buffer (Biolegend, Cat No.421002) for 30mins ...
-
bioRxiv - Cancer Biology 2023Quote: ... 2 X10^5 cells were then resuspended to be stained with 5ul of Annexin V-FITC (#640945, Biolegend) in 100ul of Annexin V binding buffer (10mM HEPES ...
-
bioRxiv - Immunology 2022Quote: ... Cells were then incubated in 2% FCS/PBS solution containing anti-mouse CD16/32 antibody (BioLegend clone 93) for 15 minutes and subsequently stained with a combination of fluorophore-conjugated primary antibodies against mouse CD45 (BioLegend clone 30-F11) ...
-
bioRxiv - Bioengineering 2022Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...
-
bioRxiv - Cancer Biology 2022Quote: ... 2% FCS and 0.09% NaN3) and stained with fluorophore-conjugated antibodies (CD146-APC, SHM-57 or P1H12, BioLegend), (EPCAM-FITC ...
-
bioRxiv - Immunology 2023Quote: ... Supernatants were collected after 24h and extracellular IL-2 was measured using the Biolegend ELISAMax Deluxe Kit (Biolegend), according to manufacturer instructions.
-
bioRxiv - Immunology 2024Quote: ... 0.25 x 106 cells were stimulated with either plate-bound 11-CD3 (BioLegend; RRID: AB_11149115; 2 µg/mL) and plate-bound 11-CD28 (BioLegend ...
-
bioRxiv - Genomics 2024Quote: ... and resuspended in 50ul PBS plus 2% FBS containing 1:100 diluted anti-CD69-APC antibody (BioLegend 310909). Cells were stained in the dark on ice for 30 min ...
-
bioRxiv - Immunology 2024Quote: ... The culture medium was supplemented with 10 ng/mL IL-2 and 25 ng/mL IL-15 (Biolegend). On the next day ...
-
bioRxiv - Immunology 2024Quote: ... The pooled sample was then stained with diluted TotalSeq-C cell surface marker antibodies (BioLegend, Supplementary Table 2) in PBS containing 2% FBS for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... Red blood cells were then removed with a 2-minute incubation in RBC Lysis Buffer (10X) (BioLegend 420301). The solution was centrifuged again at 300 x g ...
-
bioRxiv - Immunology 2021Quote: ... Nonspecific binding was blocked by incubation for 10 min at 4 °C with anti-CD16/CD32 antibody (Biolegend, clone 93, 5 ng/ μL) antibody and stained with the appropriate antibodies for 15 min at 4 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... blotted membranes were blocked with 5% milk in TBST for 30 minutes and probed overnight (4°C) with primary antibody (anti-β-actin [Sigma-Aldrich, A5441], anti-HA [Biolegend, 901501]). After 3 washes with TBST ...
-
bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
-
bioRxiv - Neuroscience 2020Quote: ... 0.05% Sodium Azide in PBS) and incubate overnight at 4°C with the monoclonal anti-amyloid beta antibody 6E10 (Biolegend, 803001, lot. B228658) at a concentration of 3μg/ml (Antibody buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
-
bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 30 min at 4°C with pre-conjugated fluorescent labeled antibodies with the following combinations: CD45 (BioLegend, 368532 (PE/Cyanine7)) ...
-
bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
-
bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
-
bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
-
bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
-
bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
-
bioRxiv - Immunology 2024Quote: ... Surface proteins were then stained at 4°C for 30 minutes using the following markers: NIR Zombie dye APC-Cy7 (Biolegend, Cat No. 423105), CD20 PE (Biolegend ...