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Citations for BioLegend :
1701 - 1750 of 2365 citations for 6H Furo 2 3 b pyrrole 5 carboxylicacid 6 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... GolgiPlug™ and GolgiStop™ were added 3 h before cells were processed for surface and intracellular staining for CD4 (BV785-conjugated anti-human; OKT-4, BioLegend), CD8 (APC-H7-conjugated anti-human ...
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bioRxiv - Immunology 2020Quote: ... mice were anesthetized with Ketamine/Xylazine (Patterson Veterinary) and injected intravenously with 3 μg of fluorophore-labelled anti-CD45 (30-F11, Biolegend) monoclonal antibody through the retro-orbital sinus to label intravascular leukocytes ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... The cells were then washed with ice-cold FACS Buffer (1x PBS + 3% FBS) before staining with mouse anti-HA (diluted 1:200, Biolegend) for 30 minutes on ice ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... The MNC fraction was depleted of red blood cells by lysis steps involving 3 incubations at room temperature for 20 mins/10 mins/10 mins respectively with RBC lysis buffer (BioLegend). Tissue lymphocytes were isolated from Peyer’s patches in intestinal mucosa using laser capture microdissection and subjected to protein lysis as outlined above ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell viability was assessed by flow cytometry after 3 days of drug or vehicle treatment using Helix NP NIR viability dye (BioLegend). Dose-response curves were fitted through interpolation using GraphPad Prism v.7 nonlinear regression fit ([inhibitor] versus normalized response–variable slope).
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bioRxiv - Synthetic Biology 2021Quote: ... incubated at a concentration of 0.1 µg/mL in HF for 3 minutes at room temperature or Zombie NIRTM (BioLegend, 423106) incubated in PBS at a 1:500 dilution for 15 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit) at 1:100 dilution (for immunodepletion) or 3 μg/ml of each 4G8 and 6E10 anti-Aβ antibodies (Biolegend) (for brain-derived Aβ enrichment ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... THP-1 and SKOV-3 cell lines and primary human T cells were labeled with fluorescent antibody against human CD70 or isotype control (Biolegend) to verify antigen expression as described before35.
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bioRxiv - Cell Biology 2023Quote: ... samples were stained for proliferation marker Ki-67 Alexa Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour and nuclei counterstain bisbenzimide (1:1000 dilution in 1X dPBS ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2019Quote: ... The slides were then stained with a rabbit primary anti-cytokeratin 5 (KRT5) antibody (Covance/BioLegend) diluted 1:500 in blocking buffer for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... IL-17A (capture Ab 5 µgml−1 TC11-18H10.1; detection Ab 2,5µgml−1 TC11-8H4, Biolegend); IFNɣ (capture Ab 5 µgml−1 AN18 ...
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bioRxiv - Immunology 2020Quote: ... supernatant was collected at day 1 and 5 for LEGENDplexTM Human T Helper Cytokine Panel (BioLegend).
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bioRxiv - Cell Biology 2020Quote: ... treated cells were harvested and stained with 5 μg/ml propidium iodide and Annexin V (Biolegend) in Annexin V-binding buffer (10 mM Hepes pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... Th17 cells were differentiated in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
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bioRxiv - Systems Biology 2021Quote: ... centrifuged and resuspended in 5 ml of 1X red blood cell (RBC) lysis buffer (420301, BioLegend) for 5 min on ice ...
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bioRxiv - Immunology 2020Quote: ... Enriched CD4SP thymocytes were then stained with CD4-Pacific blue (RM4-5) fluorochrome-conjugated antibodies (BioLegend). FACS Aria IIu and FACS Aria II SORP (BD Biosciences ...
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bioRxiv - Genetics 2020Quote: Antibodies to the following antigens were used for flow cytometry: CD4 BV650 (RM4-5, #100545, Biolegend), CD8a PE-Cy7 (53-6.7 ...
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bioRxiv - Immunology 2022Quote: ... T cells were labeled with 5 µM carboxyfluorescein diacetate succinimidyl (CFSE Cell Division Tracker Kit, Biolegend). For Treg induction and T cell activation ...
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bioRxiv - Cell Biology 2022Quote: ... BV711 Mouse anti mouse CD64 (Monoclonal; clone X54-5/7.1; Biolegend; 1:100; Cat#139311, FACS). BV421 Rat anti mouse CD68 (Monoclonal ...
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bioRxiv - Genomics 2022Quote: ... and extracellular stains with anti-mouse CD4-APC (1:400, clone RM4-5, BioLegend Cat#100516), anti-mouse TCRb chain-FITC (1:400 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained for 15 min in the dark with 5 μL APC Annexin V (Biolegend) and 5 μL 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... the cell suspension was incubated in 5 μgml−1 anti-CD16/32 (#101302, Biolegend, 1:200), stained with Live Dead Near IR (#L10119,ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... cells were first stimulated for 5 hours with either anti-CD3 antibodies (1 ug/mL, Biolegend) or MTB300 peptide megapool (21 ...
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bioRxiv - Immunology 2023Quote: ... 5 × 105 NPCs were labeled with viability dye marker (Zombie Aqua, Biolegend, San Diego, CA, USA) and blocked with PBS supplemented with 1% Fc Block (MACS ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were stained with 5 ul each of PE-anti human CD45 antibody clone HI30 (Biolegend) and APC-anti human CD11b antibody clone ICRF44 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 96-well plates were pre-coated with 5 μg/ml anti-hCD3 (BioLegend, Cat# 317326, RRID:AB_11150592), 1 μg/ml anti-hCD28 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa Fluor 488 anti-CD54 antibody (2 μg/mL, ICAM-1, YN1/1.7.4, BioLegend), and Alexa Fluor 647 anti-CD106 antibody (2 μg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... we used an antibody against the Rpb3 subunit (2 μl per reaction, Biolegend 665004) conjugated to 20 μl protein G magnetic beads (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were plated at 2 million cells/well and incubated with Zombie UV (Biolegend) for dead cell discrimination ...
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bioRxiv - Immunology 2019Quote: ... Culture supernatants were collected and analyzed for IFNg and IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2021Quote: ... 0.1% 2-mercapto-ethanol 1000X solution) supplemented with IL-7 (10 ng/mL, Biolegend). To activate T cells ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then washed with 2 ml of cell staining buffer (Cat. #420201, BioLegend), resuspended in 300 μl PBS and measured using an LSR II flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells (2 x 105) were incubated with PerCP-anti-CD4 (clone A161A1) antibody (Biolegend) for 15 min at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... These microglia were labeled with FITC anti-mouse CD45 (2 µg/mL, Biolegend, USA), APC anti-mouse CD11b (5 µg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Cell Biology 2021Quote: ... EVs were then pulled down by incubating with anti CD81-Biotin (Eat-2, Biolegend) coated streptavidin beads overnight rotating at 4°C (Exobeads ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 μM (in vitro) and neutralizing HMGB1 of 10µg/ml were obtained from Biolegend (isotype control mouse IgG2a kappa ...
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bioRxiv - Immunology 2020Quote: ... supernatants were harvested and TNF and IL-2 levels were measured by ELISA (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: 2 µg of primary conjugated anti-mouse Ly6G-Alexa Fluor 647 (clone 1A8, Biolegend) diluted into 200 µl PBS were injected intravenously via tail vein ten minutes prior to imaging as previously published [15,16].
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with PBS/2% FBS and stained with streptavidin conjugated BV605 (BioLegend) if CD90 biotin-conjugated antibody was used ...