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Citations for BioLegend :
1701 - 1750 of 7911 citations for 6 4 METHOXY PHENYL 2 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... KLRG1 APC-Cy7 (clone 2FI/KLRG1, BioLegend, cat# 138426, 2:100 dilution); CD279 (PD-1 ...
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bioRxiv - Neuroscience 2022Quote: ... Fisher) along with 2 µl of live/dead cell stain (DRAQ7, BioLegend) per ml and then FACS sorted.
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bioRxiv - Immunology 2022Quote: ... Rat anti-CD138 antibody (rat anti-CD138, clone 281-2, Biolegend, 142502) was applied to each section and specific bounds were visualized using a Alexa Fluor488-conjugated anti-rat antibody (JacksonImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend, 148603) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were collected and analyzed for IL-2 by ELISA (BioLegend).
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bioRxiv - Immunology 2023Quote: ... Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS; BioLegend) and incubated with PGP9.5 for 20 min at RT ...
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bioRxiv - Immunology 2024Quote: ... and with 100 U/ml IL-2 (BioLegend, San Diego, CA, USA) (referred to as “Bead activated T cells” or “nonspecific activation” ...
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bioRxiv - Immunology 2024Quote: ... the gels were incubated with 2 μg/ml Calcein-AM (BioLegend, 425201) and 1 μg/ml Propidium Iodide (MP Biomedicals ...
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bioRxiv - Immunology 2024Quote: ... Media was additionally supplemented with 10 ng/mL murine IL-2 (BioLegend) prior to resuspension and subsequent passaging ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Immunology 2021Quote: ... cells were incubated overnight at 4 °C with the primary antibodies against ASC (HASC-71, BioLegend). Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20 ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation (“in vitro-derived”) were washed and labeled using CFSE (BioLegend). Murine bone marrow neutrophils were isolated by negative selection (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... After 4 hours BMDMs were harvested and stained with an antibody against F4/80 (BM8, Biolegend) and propidium iodide (BD Biosciences) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse B220 (Biolegend, APC), GL7 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were incubated for 15 minutes at 4°C in streptavidin-PE/Cy7 (BioLegend) to detect NCAM-biotin ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were first incubated for 1h at 4°C with 2μl of anti- HA antibody (BioLegend) and then for another hr after adding 30μl of washed magnetic ProteinG beads (Cytiva) ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Cells were then incubated for 10 minutes at 4°C with True stain Fc block (BioLegend) and washed ...
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bioRxiv - Immunology 2019Quote: ... macrophage activation was induced by adding either IL-4 (20 ng/ml, Biolegend, San Diego, USA), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight staining at 4°C with rat anti-mouse CD45 primary antibody (103102, Biolegend) diluted 1:500 in 1x D-PBS ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend) were added to the neutralized FA fraction ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was removed by aspiration and 500 microliters of fixation buffer (4% paraformaldehyde in PBS, Biolegend) added to each well and the slide kept in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and stained (30 min at 4°C) with PE anti-TLR4 (Biolegend; clone Sa15-21; 145404) in blocking buffer (PBS with 2% rat serum) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cd49b-AF488 (Biolegend, clone HMα2, 1 µg ml−1, 1:500) and Sca1-AF647 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... flotillin-1 (W16108A; 1:1000; Biolegend), peIF2a (D9G8 ...
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bioRxiv - Cell Biology 2021Quote: ... Sca-1 (BioLegend, # 108126; 1:200), Ter119 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1 (Biolegend, 101226, 1:400), B220 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... CD48 (HM48-1; Biolegend; 1:100), CXCR4 (2B11 ...
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bioRxiv - Cell Biology 2020Quote: ... -CD48 (HM48-1, Biolegend, 1:100), -CD150 (TC15-12F12.2 ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng·ml−1 mCSF-1 (BioLegend), and 5 μg·ml−1 plasmocin (Invivogen ...
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bioRxiv - Developmental Biology 2023Quote: β1-Alexa647 (1:500, BioLegend, 102214)
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bioRxiv - Genetics 2021Quote: ... production from LPS- or OL-treated BMDM were performed using Mouse TNF-α ELISA MAX Deluxe kit and Mouse IL-6 ELISA MAX Deluxe kit (BioLegend, San Diego, CA, USA). Follow-up cytokine (IL1β ...
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bioRxiv - Systems Biology 2021Quote: ... for 5 min to block Fc receptors and then stained with antibodies against CD11b (BioLegend), Ly6C (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with oligonucleotide-tagged anti-VCAM1 and anti-CD24 antibodies (BioLegend, Supplementary Table 5) on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... tissue culture plates were coated with 5 μg/mL of anti-human CD162 (KPL, Biolegend). On day three of activation ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained for 30 minutes with anti-mouse CD45-PerCP-Cy5-5 (103132; BioLegend), PDPN-APC/Cy7 (127418 ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell pellets were resuspended in 5 mL of Red Blood Cell (RBC) lysis buffer (BioLegend) and incubated at room temperature for 10 minutes ...
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bioRxiv - Genetics 2019Quote: ... and then stained with anti-FOXP3-BV421 antibody (5 µl/106 cells, clone 206D, BioLegend) using the eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... NK cells were pre-incubated with CD56 Alexa Fluor 488 (HCD56, Biolegend, 5 μg/ml). Following incubation ...
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bioRxiv - Developmental Biology 2021Quote: ... and rat anti-lineage cocktail (labelled with BV421) (BioLegend – 133311; 5 µl per million cells) in staining buffer and washed twice ...
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bioRxiv - Bioengineering 2022Quote: ... and 5 μL of 100 μg/mL anti-CD8 Alexa Fluor 488 (Biolegend, Catalog: 344716) per 6 μL nanovial volume for 30 minutes in a 37°C incubator ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for >5 minutes at 4C with Fc Block (clone 93; BioLegend), then stained in the presence of Fc block for 30 minutes at 4C using the following antibodies ...
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bioRxiv - Cancer Biology 2019Quote: ... YAP1 was co-stained with cytokeratin 5 (CK5) or 14 (CK14) (BioLegend, San Diego, CA) and imaged with a Nikon fluorescence microscope (Melville ...
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bioRxiv - Immunology 2020Quote: ... tissue-culture plates were coated with 5 μg/mL purified anti-CD3 (clone HIT3a, Biolegend) at 4°C overnight and subsequently washed twice with 1X PBS ...
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bioRxiv - Immunology 2020Quote: ... Fc receptors were first blocked for 5 minutes using CD16/CD32 (Biolegend, San Diego, CA). Eosinophils were identified as CD11c-Siglec-F+ and neutrophils were identified as SiglecF-GR-1+ ...
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bioRxiv - Immunology 2021Quote: ... Sorted transduced macrophages were stimulated with 5 ng/mL recombinant murine IFN-γ (575304, Biolegend) 12-14 hours before stimulation with 10 ng/mL recombinant TNFα as described above for 4 hours (FBS used in these experiments was from Omega ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were stained with 2.5ug of anti-CD4-APC (RM4-5, Biolegend 100516) antibody in 1mL of sort buffer and incubated at 4°C for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 5×105 SABR expressing NFAT-GFP-Jurkat cells were labeled with cell trace violet (BioLegend) according to the manufacturer’s protocol before incubation with 5×105 TCR expressing Jurkat cells in a round bottom 96-well plate for 16-20 hr ...
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bioRxiv - Physiology 2022Quote: ... and then stained for subsets of markers at 5 μg/ml: Ly6G-BV421(Biolegend, 127628), Ly6C-BV605 (Biolegend ...