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Citations for BioLegend :
1651 - 1700 of 2954 citations for RSPO3 Human HEK293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... anti-human HLA-A,B,C (clone W6/32, BioLegend), anti-human CCR7 (clone 3D12 ...
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bioRxiv - Biophysics 2024Quote: ... antibodies Alexa Fluor 700 anti-human CD63 (BioLegend, cat. 353024) and PE anti-human CD9 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa 647 anti-human CD45 (1:400; HI30 Biolegend 304056), rabbit anti-human/mouse cleaved-caspase 3 (1:300 ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2021Quote: ... CAR expression analysis was performed using incubation with His-tagged recombinant CD123 protein (SinoBiological, Beijing, China) and secondary staining with αHis-PE or αHis-APC (BioLegend, San Diego, CA). Dead cells were excluded from analysis using LIVE/DEAD Fixable Viability Stains 780 or 575V (BD Horizon ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
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bioRxiv - Immunology 2021Quote: Mouse B cells were bulk sorted from mixed chimeras into Eppendorf tubes containing cold sterile PBS 10% FCS and incubated for 20 min at 4°C with TotalSeq™ DNA-barcoded anti-mouse ‘Hashing’ antibodies (BioLegend) at a 1/100 final dilution ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellets washed twice with 10% FCS in PBS (100μl/well) and then resuspended in 10% FCS in PBS (100μl/well) containing APC/Cyanine7 Goat anti-mouse IgG (Biolegend; 1:250) and incubated on ice for 30mins ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Immunology 2019Quote: The percentage of CAR and ΔLNGFR positive T cells was assessed by biotin-conjugated rhCD19-Fc (Cat # CD9-H5259, ACRO Biosystems, USA) with AF647-conjugated streptavidin (Cat # 405237, Biolegend, USA), and fluorescein isothiocyanate (FITC)-conjugated LNGFR antibody (Cat# 130-112-605 ...
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bioRxiv - Immunology 2020Quote: Fc block was performed using TruStain FcX™ CD16/32 antibody (anti-mouse) to avoid non-specific binding (101320 Biolegend, UK) according to the supplier protocols The fluorochrome-conjugated antibodies used in the panel were obtained from Biolegend ...
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bioRxiv - Immunology 2022Quote: The following protocol was used for all tumor experiments: after the Fc receptor of the cells was blocked with anti-mouse CD16/32 (Biolegend, 101320), extracellular staining was performed in FACS buffer for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and preincubated for 15min with anti-CD16/CD32 Fc-block antibody (10μg/ml; BioLegend, San Diego, CA, 1:50) in FACS buffer followed by a 30min incubation with fluorescence dye-conjugated ...
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bioRxiv - Immunology 2023Quote: ... and stained with a master mix of antibodies, viability dye (Ebioscience, Cat# 65-0865-14) and Fc block (Biolegend, Cat#422302) in FACS buffer for surface staining ...
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bioRxiv - Physiology 2023Quote: ... Cisplatin is washed off and non-specific binding was blocked with a 10-minute staining of cells with Fc Block (Biolegend; 1µg). Cells were stained with 0.5µg of cell surface metal-conjugated antibodies for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: Murine DCs were differentiated from isolated bone marrow via culture in RPMI + 10% FCS 1% Pen/Strep and 1% Amino Acids containing 20 ng/ml GMCSF (Biolegend, #576306) on silicone hydrogels with different stiffnesses ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Cell Biology 2024Quote: Single-cell suspensions were pre-incubated with 1 µg/mL of aCD16/32 Fc-Block (BioLegend, San Diego, CA, USA, 101310) in 300 µL staining buffer for 10 min ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed at a dilution of 1:100 for 25 minutes at 4L°C in the presence of mouse Fc block (TruStain FcX™, anti-mouse CD16/32, BioLegend) in PBS containing 5% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... Cytokines were determined by fluorescent bead-based multiplex sandwich immunoassays (LEGENDplex™ Human Angiogenesis Panel 1, 740697 and LEGENDplex™ Human Adipokine Panel, 740196; BioLegend, Inc., San Diego, CA) read by flow cytometry (Guava easyCyte 8HT ...
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bioRxiv - Immunology 2021Quote: Recombinant human ARG1 was obtained from Biolegend (San Diego, CA, USA), recombinant murine Arg1 was obtained from Cloud-Clone Corp. ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD123 BV510 (1:100, clone 6H6, BioLegend, cat # 306022), Anti-human CD34 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD69 BV650 (1:100, clone FN50, BioLegend, cat # 310934), Anti-human CD8a BV650 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TCRgd BV510 (1:100, clone B1, BioLegend, cat # 331220), Anti-human TCRab BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD56 BV605 (1:50, clone HCD56, BioLegend, cat # 318334), Anti-human CD69 BV650 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD13 BV421 (1:50, clone WM15, BioLegend, cat # 301716), Anti-human TCRgd BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD3 BV510 (1:100, clone OKT3, BioLegend, cat # 317332), Anti-human CD14 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD3 FITC (1:100, clone UCHT1, BioLegend, cat # 300440), Anti-human CD8a PerCP (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD107a BV750 (1:50, clone H4A3, BioLegend, cat # 328638), Anti-human CD103 BV711 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TCRgd FITC (1:100, clone B1, BioLegend, cat # 331208), Anti-human CD3 FITC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD326 BV650 (1:100, clone 9C4, BioLegend, cat # 324226), Anti-human CD107a BV750 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CXCR3 BV421 (1:50, clone G025H7, BioLegend, cat # 353716), Anti-human CD13 BV421 (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD20 BV510 (1:100, clone 2H7, BioLegend, cat # 302340), Anti-human CD3 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CCR6 APC (1:25, clone G034E3, BioLegend, cat # 353416), Anti-human CD206 APC (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD34 BV510 (1:100, clone 581, BioLegend, cat #343528), Anti-human CD20 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD45 BV711 (1:100, clone HI30, BioLegend, cat # 304050), Anti-human CD3 BV785 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CCR8 PE (1:100, clone L263G8, BioLegend, cat # 360604), Anti-human CD127 PE (1:50 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD14 BV510 (1:100, clone M5E2, BioLegend, cat # 301842), Anti-human CD19 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD19 BV510 (1:100, clone HIB19, BioLegend, cat # 302242), Anti-human CD4 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD5 BV510 (1:100, clone L17F12, BioLegend, cat # 364018), Anti-human FcER1 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD303 BV510 (1:100, clone 201A, BioLegend, cat # 354232), Anti-human CD123 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... biotin anti-human CD19 (BioLegend, San Diego, CA, USA, Cat. 302203) was used as a primary stain ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD10 BV711 (1:100, clone HI10a, BioLegend, cat # 312226), Anti-human CD45 BV711 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human TCRab BV510 (1:100, clone IP26, BioLegend, cat # 306734), Anti-human CD5 BV510 (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Anti-human CD3 BV785 (1:100, clone OKT3, BioLegend, cat # 317330), Anti-human HLA-DR BV785 (1:50 ...