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Citations for BioLegend :
1651 - 1700 of 8621 citations for 6 METHYL 7 1 METHYL 2 OXO PROPOXY 2 3 DIHYDRO 1H CYCLOPENTA C CHROMEN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 2 mice were used whose cells were labelled with Hashtags oligos (HTO) using the Cell Hashing protocol from Biolegend (TotalSeq-A). All the sorted cells were pooled on the same tube and processed in the Chromium Next GEM Chip G using Chromium Next GEM Single Cell 3’ v3.1.
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40592-V27H-B) conjugated with APC-streptavidin (BioLegend, 405207), and 5 μL 7-AAD (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... for 30min at 4 °C and intracellular markers (BV421-labeled anti-mouse IFN-γ, Cat# 505830, BioLegend; FITC-labeled anti-mouse IL-2, Cat# 503806, BioLegend; BV711-labeled anti-mouse TNF-α ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2019Quote: ... PBMCs were then thawed and plated in 24-well plates (2-2.5 millions/1ml/well) pre-coated with anti-CD3 (2.5 ug/ml, HIT3a clone, Biolegend, San Diego, CA) for 24 hours before harvesting ...
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bioRxiv - Immunology 2020Quote: ... CD19-CAR moiety was labeled by AF647-conjugated anti-mouse F(ab’)2 antibody or biotin-conjugated rhCD19-Fc (ACRO Biosystems, USA) with AF647-conjugated streptavidin (Biolegend, USA). ΔLNGFR was labeled with APC-conjugated LNGFR antibody (clone ME20.4-1.H4 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were centrifuged at 1800 rpm for 5 mins and then incubated in 2 μg/mL solution of anti-CD11b antibody (Biolegend 101257) and anti-GR1 antibody (Biolegend 108422 ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to 2 ml tubes for incubation with the following antibodies diluted in media containing FBS: CD45-PE/Cy7 (BioLegend, 103114), ICAM2-A647 (Life tech ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Sixteen micron sections were cut and blocked in .01% Triton X 100 with 2% of FBS and donkey serum including TrueStain Monocyte Blocker (BioLegend 426101) for 1h and then stained overnight with the following directly conjugated mAbs ...
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... Skin immune cells were subsequently added to each fibroblasts-placed well and co-cultured in the presence of 20 ng/ml human IL-2 recombinant and 20 ng/ml human IL-15 recombinant (BioLegend, 570304) in 24 well plates (Ratio of 50:1 immune cell-to-fibroblast) ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Immunology 2023Quote: Levels of CXCL9 and CXCL10 were measured in plasma (diluted 2-fold) using the mouse cytokine release syndrome panel LEGENDplex system (BioLegend, UK), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... Nanovials were reconstituted at a ten-fold dilution in Washing Buffer containing 2 μL of 100 μg/mL anti-HLA-A2 FITC antibody (Biolegend, 343304) and incubated for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Neuroscience 2023Quote: Endothelial cells isolated from brain from SARS-CoV-2 infected mice were stained with fixable viability dye (Zombie Green, Biolegend 423111) and blocked with Fc receptor blockade (anti-mouse CD16/32 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend, #317304) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).
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bioRxiv - Immunology 2024Quote: ... single cell suspension was incubated with the cell hashtag antibodies (unique DNA barcodes listed in Supplementary Table 2) and the cocktail of 119 unique cell surface antibodies (Biolegend, #199901), as per manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... cells were stained with Pacific Blue-Annexin V and 7AAD (7-aminoactinomycin D) (Biolegend) before running in an LSRII cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD15 antibody (phycoerithrin-cyanine 7 conjugated mouse anti-human, clone W6D3, BioLegend) to identify neutrophils ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined using flow cytometry and 7-AAD Viability Staining Solution (Biolegend). Cell counts for all fractions were ascertained using the Countess cell counter (Invitrogen).
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bioRxiv - Neuroscience 2021Quote: ... For dead cell exclusion a 7-AAD viability staining solution was employed (Biolegend, 420403) (5 µL per million cells) ...
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bioRxiv - Cancer Biology 2024Quote: ... lymphoma cells were stained with 7-AAD Viability Staining Solution (cat. no. 420404, Biolegend) and Annexin V (cat ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in FACS buffer containing 0.5 µg/mL 7-AAD (Biolegend, 420403) and incubated on ice for 10 minutes and then acquired directly on the Attune NxT flow cytometer (Thermofisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5 μL of 7-AAD and 2.5 μL of FITC-Annexin V (Biolegend #640922) were added ...
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bioRxiv - Microbiology 2023Quote: ... Dead cells (identified using 7-AAD or Zombie Fixable Viability Kits; both from BioLegend) and cell aggregates (identified on FSC-A versus FSC-H scatter plots ...
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bioRxiv - Immunology 2022Quote: ... FITC Annexin V Apoptosis Detection Kit with 7-AAD (#640922) was purchased from BioLegend.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend). Cell counts were ascertained using the Countess (Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 µL of 7-AAD and 2.5 µL of FITC-Annexin V (Biolegend #640922) were added ...
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bioRxiv - Immunology 2022Quote: ... Tim-3 was either neutralized by anti-human Tim-3 antibodies (10 µg/mL) (Biolegend) for 1 h prior to treatment or through knockdown via duplexed siRNA (Integrated DNA Technologies ...
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bioRxiv - Immunology 2022Quote: ... and Mouse IL-6 ELISA MAXTM Deluxe Set (Biolegend) according to the manufacturer’s protocol ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... and stained with FITC-labelled anti-IL-6 (BioLegend). Acquisition was performed on a FACSVerse flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... or 6 μg/mL anti-ICOS (clone C398.4A, Biolegend) in PBS overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... clone HI9a (6 µg/mL, BioLegend, San Diego, USA); anti-CD63–APC ...
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bioRxiv - Immunology 2020Quote: ... anti-MHC class I (clone 28-8-6, BioLegend), anti-β-catenin (clone D2U8Y ...
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bioRxiv - Immunology 2024Quote: ... and cytokine stains with IL-6 (MP5-20F3; Biolegend), IL-10 (JES5-16E3 ...
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bioRxiv - Bioengineering 2024Quote: ... Magnetic beads displaying recombinant IL-6 protein (BioLegend #570806) were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203) ...
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bioRxiv - Immunology 2020Quote: ... c-kit-BV605 (104D2; BioLegend), CRTH2-PE (MACS Milltenyi Biotec) ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-c-Myc (9E10, Biolegend), anti-V5 (AbD Serotec) ...
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bioRxiv - Immunology 2021Quote: ... TotalSeq-C Hashtag antibodies (Biolegend) were used to multiplex samples from different donors ...