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Citations for BioLegend :
1651 - 1700 of 2141 citations for 6 FLUORO 2 METHYL 3 4 PIPERIDINE 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were fixed with 4% PFA for 10 min at RT followed by permeabilization with the True-Phos Perm buffer (Biolegend) for 20 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody staining was carried out at 4°C overnight with the following antibody dilutions in blocking buffer: CD52-APC (clone HI186, BioLegend) 1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixation and permeabilization was then performed for 1 hour at 4°C using the CytoFast Fix/Perm Set (426803, Biolegend). After fixation/permeabilization ...
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bioRxiv - Immunology 2024Quote: DCs were differentiated from bone marrow precursors obtained from naive BALB/c mice in the presence of murine GM-CSF (10 ng/ml) and IL-4 (10 ng/ml) both from Biolegend for 7 days ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 30 min at 4°C with 1 µL of PE anti-human FasL antibody (clone NOK-1) from BioLegend. Nonspecific background signals were measured by incubating cells with the appropriate isotype-matched antibody ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h and incubated O/N at 4°C with primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), hnRNP A2/B1 (sc-374053 ...
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bioRxiv - Immunology 2024Quote: ... single cells from the omentum tumor were incubated for 4 hours with Cell Activation Cocktail (with Brefeldin A) (catalog no. 423303, Biolegend) prior to staining.
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
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bioRxiv - Immunology 2024Quote: Mitochondrial mass was determined in DCs by fixing the cells with PFA 4% and labeling them with the probe MitoSpy Green FM (Biolegend). Green fluorescence was analyzed by flow cytometry (FACScan ...
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bioRxiv - Immunology 2024Quote: ... cells were labeled stained in PBS for 20 mins at 4°C with a live dead marker (Zombie UVTM, Biolegend). Surface staining was performed in brilliant stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Immunology 2021Quote: ... Mouse IL-2 was measured in the supernatants by enzyme-linked immunosorbent assay (BioLegend, BD Biosciences).
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bioRxiv - Cancer Biology 2019Quote: ... and 2×106 cells were incubated with mouse anti-HLA class I antibody (1:200, BioLegend) for 30min followed by secondary antibody incubation for 30min ...
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bioRxiv - Immunology 2021Quote: ... 18 μL of PHA (1 μg/μL stock) and 2 μL of Cell activation cocktail (Biolegend) are added to the tube ...
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bioRxiv - Immunology 2021Quote: ... the cells were lysed and fixed with 2 ml of 1x RBC Lysis/Fixation Solution (Biolegend) for 15 minutes at RT and washed with cold FACS-buffer (PBS with 1 % FCS) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed twice with 1x PBS + 2% FBS and fixed with Fluorofix Buffer (BioLegend) at RT for 30 minutes in the dark with shaking ...
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bioRxiv - Microbiology 2021Quote: ... and mouse anti-human BST-2 (clone RS38E, PE-Cy7-conjugated; Biolegend, San Diego, CA, USA) were also used as primary antibodies for cell-surface staining ...
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bioRxiv - Immunology 2022Quote: ... Cells were then stained with intracellular monoclonal antibody-fluorochrome conjugates: IL-2-PE (BioLegend, cat.# 500307), TNF-α-FITC (BD ...
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bioRxiv - Cancer Biology 2022Quote: 1–2 × 106 NB4 treated cells were harvested and labelled with FITC-conjugated Annexin-V (Biolegend) for 15 min according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2022Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. For analyzing intracellular cytokine IFN-γ and IL-17A ...
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bioRxiv - Cancer Biology 2022Quote: ... TGFß (1 ng/ml; cat#763102) and IL-2 (5 ng/ml, cat# 575402; All Biolegend) were added along with T cell media (RPMI ...
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bioRxiv - Immunology 2020Quote: ... for 30min followed by staining with αLFA1-Alexa Fluor647 (Clone: CBR LFA 1/2, BioLegend, 366311) for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2021Quote: ... the following probes were generated: SARS-CoV-2 RBD-APC/Cy7 (streptavidin-APC/Cy7 from BioLegend), SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences) ...
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bioRxiv - Immunology 2022Quote: ... the fixed samples were stained with the following antibodies: PE-Cy7-labeled CD63 (NVG-2, BioLegend), Percp-eFluor 710-labeled iNOS (CXNFT ...
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bioRxiv - Molecular Biology 2022Quote: ... Splenocytes were stimulated for 42 hours at 37°C before adding 2 mM monensin (Biolegend #420701) for an additional 6 hours ...
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bioRxiv - Immunology 2020Quote: ... antibody was purchased from BD Biosciences (San Jose, Calif.) and the anti-CD206 antibody (clone 15-2) was purchased from Biolegend. Briefly ...
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bioRxiv - Immunology 2020Quote: ... and IL-2 concentrations were analyzed using the respective LEGEND MAX Human ELISA Kits (BioLegend, USA).
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bioRxiv - Cancer Biology 2021Quote: ... then fixed in 2% PFA and permeabilized using 1X Intracellular Staining Perm Wash Buffer (BioLegend; 421002) prior to intracellular staining with Vimentin (BD Pharmingen ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. Foxp3 expression was performed using the Foxp3 staining kit from eBioscience ...
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bioRxiv - Immunology 2021Quote: ... anti-CD11c (clone N418, 25-0144-82, eBioscience) and anti-CD18 (clone M18/2, 101407, BioLegend), -CD29 (clone HMβ1-1 ...
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bioRxiv - Immunology 2022Quote: ... were coated with 1 µg/ml anti-CD3 and 2 µg/ml anti-CD28 antibodies (BioLegend) for 2 hours and seeded with 50.000 T cells/well ...
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bioRxiv - Immunology 2022Quote: ... coated flat bottomed 96 well plates with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend), and with or without human recombinant soluble BAFF at 500 ng/mL ...
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bioRxiv - Synthetic Biology 2022Quote: ... mixed to a final concentration of 2 µg/mL or 60 kDa FITC-labeled streptavidin (Biolegend) to a final concentration of 50 µg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... red blood cells (RBCs) were removed by lysis in 2 mL of RBC lysis buffer (BioLegend) for 3 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Immunology 2023Quote: ... BA.1 or BA.2 biotinylated RBD protein conjugated with TotalSeq™-C0971 Streptavidin (BioLegend, 405271) and TotalSeq™-C0972 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2023Quote: Antigen-specific B cell subsets were detected using biotinylated SARS-CoV-2 RBD protein (793906; Biolegend) in combination with streptavidin (SAv)-fluorophore conjugates as described [7] ...
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bioRxiv - Cell Biology 2022Quote: ... PI (1:100) and cell hashing antibodies (1:1000, #1 for heart, #2 for blood, Biolegend) and incubated for 30min protected from light ...
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were resuspended in PBS containing 2 % BSA and a viability dye (Zombie Red, Biolegend) for discrimination between live and dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with 2 ug/ml PE/Cyanine7-conjugated anti-mouse TLR4 antibodies (BioLegend, 145407) or 2 ug/ml PE-conjugated anti-mouse TLR2 antibodies (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... A total of 2 × 105 donor-matched PBMCs or ToMCs were labeled with CFSE (BioLegend #423801), pre-treated with different inhibitors for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... immunoprecipitation was conducted for the supernantant as described above using 2 µg of M3.2 antibody (Biolegend) and 50 µl Protein G sepharose.
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bioRxiv - Immunology 2023Quote: IL-2 concentrations in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) from BioLegend. For the CRISPR screen ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was used to determine the IL-2 secretion using the IL2 Elisa kit (#BioLegend), and the cells were used to determine the protein concentration using BCA assay (#Thermo ...
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bioRxiv - Immunology 2024Quote: ... Supplementary Table 1) and 1ul/staining volume of hashtag TotalSeqC antibody mastermix (Biolegend, Supplementary Table 2) was added to each sample and cells were incubated for 30 minutes at 4°C ...