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Citations for BioLegend :
1601 - 1650 of 3392 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... CD138 (APC anti-human CD138 Antibody, Biolegend, USA, 1:100), or CD45 (FITC Antibody ...
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bioRxiv - Immunology 2024Quote: ... 0.25 μg 5×106 PBMCs anti-human CXCR3 (BioLegend, 353251)) were added ...
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bioRxiv - Immunology 2024Quote: ... and anti-human CD15 (SSEA-1)(Alexa Fluor 488) (Biolegend). Data were analyzed with FlowJo software ...
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bioRxiv - Immunology 2024Quote: ... and anti-Human CD3-FITC (1:100, Biolegend, clone SK7) for 30 minutes at 4 °C ...
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bioRxiv - Genomics 2024Quote: ... and anti-CD3 (PE/Cyanine7 anti-human CD3 Antibody, Biolegend) antibodies for 20 minutes at 4 ºC protected from light ...
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bioRxiv - Immunology 2024Quote: ... and incubated with 1 % BSA Human TruStain FcX™ (BioLegend) for 20 minutes ...
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bioRxiv - Bioengineering 2024Quote: Cells were stained with anti-human B2M-PE (316306, Biolegend) in PBS containing 1% bovine serum albumin ...
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bioRxiv - Cancer Biology 2024Quote: ... APC anti-human ABCG2 antibody (Biolegend, clone 5D3, cat#332020), PE anti-human ABCB1 Antibody (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... PE anti-human ABCB1 Antibody (Biolegend, clone 4E3.16, cat#919406), FITC Mouse Anti-Human MRP1 antibody (BD Biosciences ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were first treated with Human TruStain FcX (BioLegend 422302) diluted 1:20 in PBS containing 1% BSA for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... or 1 µl APC anti-human CD22 (clone: HIB22, BioLegend) with or without 1 µl Pacific Blue anti-human CD19 (clone ...
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bioRxiv - Biophysics 2020Quote: The antibodies used for these experiments were mouse purified anti-HA.11 Epitope Tag monoclonal (clone 16B12) (Biolegend 901502; 1:500 dilution), rabbit affinity-isolated anti-FLAG polyclonal (Sigma-Aldrich F7425 ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed twice with PBSF and labeled with 50 μL of APC-conjugated monoclonal mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, Cat # 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Pathology 2022Quote: ... or B cell supernatant pre-treated with a recombinant antibody to deplete IL-10 (IgG2b, clone JES5-16E3, Biolegend). As controls ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Immunology 2024Quote: ... which is RPMI-1640-based and supplemented with 5 μg/mL recombinant mouse IL-3 (#575506 or #575508, BioLegend) as described in previous studies (6 ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Immunology 2023Quote: ... NOD female mice were injected intraperitoneally (i.p.) with recombinant mouse IL-33 (0.5-1ug/mice/day, in 100uL of PBS; BioLegend: 580508) or vehicle only (PBS ...
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bioRxiv - Immunology 2023Quote: ... Enriched CellTrace Violet-labeled OT-I CD8+ T cells were resuspended at 20,000 cells/100uL in complete RPMI + 10ng/mL of recombinant mouse IL-2 (Biolegend). As positive control ...
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bioRxiv - Immunology 2023Quote: ... CD73- CD4+ CD25- Foxp3- newly generated CD4SP thymocytes were sorted from Foxp3eGFP-Cre-ERT2 reporter mice and reconstituted at a concentration of 20,000 cells/100uL in complete RPMI + 100ng/mL recombinant mouse IL-7 (Biolegend). CD4SP thymocytes and DC subsets were co-cultured at a 2:1 ratio in round-bottom 96-well plates for 4 days and then analyzed by flow cytometry on day 5 to evaluate the percentage of Tregs generated in vitro.
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bioRxiv - Microbiology 2024Quote: ... supplemented with recombinant IL-2 (R&D Systemts) at 10ng/mL and 4µg/mL of anti-CD28 antibody (Biolegend), plated in 24-well plates coated with 2µg/mL of anti-CD3 antibody (Biolegend) ...
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bioRxiv - Systems Biology 2024Quote: ... medium was replaced by full IMDM medium supplemented with 100ng/mL of either Recombinant Mouse IL-1β (Biolegend, 575102), Recombinant Human TWEAK (Biolegend ...
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bioRxiv - Biochemistry 2024Quote: ... were pre-treated with the BLUE inhibitor panel (5 µM) or DMSO vehicle control for 1 hour followed by addition of recombinant IFNα2 (50 ng/mL, 592702, BioLegend). Following 1 hour incubation with IFNα2 ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2024Quote: HpARI proteins tetramers were prepared by mixing HpARI biotinylated proteins with PE-conjugated streptavidin (Biolegend) at a 4:1 molar ratio for 30 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
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bioRxiv - Immunology 2023Quote: For GBP2 co-adoptively transfer experiment MutuDC1-dCAS9KRAB sgNT versus MutuDC1-dCAS9KRAB sgGBP2 cells were stained with Tag-it Violet™ Proliferation and Cell Tracking Dye (Biolegend Cat.425101) or CellTrace™ Far Red Cell Proliferation Kit (Invitrogen Cat.C34564) ...
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bioRxiv - Immunology 2021Quote: ... in culture medium supplemented with 0.5 µg/mL soluble anti-mouse CD28 (eBioscience) and 10 ng/mL (∼50 U/mL) recombinant murine Interleukin-2 (IL-2, BioLegend), and activated for up to 72h ...
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bioRxiv - Microbiology 2021Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend)) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Neuroscience 2022Quote: ... neuronal activity was recorded for 15– 30 min daily before and after treatment with recombinant CXCL11 (200 nM, BioLegend #573606) using the Maestro Pro MEA System (Axion BioSystems) ...
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bioRxiv - Immunology 2024Quote: ... were cultured for 3 days in 96-well U bottom plates coated with 2 μg/mL anti-CD3 (eBioscience) and 1 μg/mL anti-CD28 (eBioscience) supplemented with 10ng/mL of recombinant mIL-2 (Biolegend) to allow Treg survival ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were activated with αCD3/αCD28 Dynabeads as specified by manufacturer’s instructions in the presence of 100 IU/mL recombinant mouse (rm) IL-2 and 10 ng/mL rmIL-7 (BioLegend) and cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated at 37°C and 5% CO2 for one hour to settle and then stimulated with 20 ng/mL recombinant IFNg (BioLegend), 100 ng/mL LPS-EB Ultrapure (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were incubated for 48h with OVAp (10 ng/ml) and mouse recombinant IL2 (50 U/ml) (Cat# 575402, Biolegend), was added for a period of five additional days ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin-streptomycin for 16 h before exposure to recombinant mouse IL-6 (rIL-6) at 50 ng/ml (575702, BioLegend) or vehicle (PBS ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 5×106 cells/mL in RPMI media with 10% FBS and 30ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF; BioLegend). After 48 hours and again after 120 hours from initial plating cell culture media was replaced with fresh media containing M-CSF ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... 6–8-week-old C57BL/6J mice were administered PBS or recombinant mouse CCL4 (400 ng/mouse, Cat. No. 554602, BioLegend) intraperitoneally at -3 ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 300 g for 5 min at 4°C and cultured in flasks at 106 cells/mL in complete RMPI with 20 ng/mL recombinant mouse IL-2 (rmIL-2; Biolegend). To generate talin-deficient T cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then seeded at a density of 0.3×106/ml with complete RPMI medium supplemented with 20ng/ml recombinant M-CSF (BioLegend, 576404), at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 250,000 cells were plated in 1 ml IMDM media with 20% FBS and 1% Penicillin Streptomycin containing 50 ng/ml recombinant mouse M-CSF (cat # 576406, Biolegend) in a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... Bone marrow cells were differentiated into BMDMs on petri dishes using 40 ng/mL recombinant M-CSF (BioLegend, Cat# 576406) and 1µg/ml 4-Hydroxytamoxifen (Millipore Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... Cytokines were determined by fluorescent bead-based multiplex sandwich immunoassays (LEGENDplex™ Human Angiogenesis Panel 1, 740697 and LEGENDplex™ Human Adipokine Panel, 740196; BioLegend, Inc., San Diego, CA) read by flow cytometry (Guava easyCyte 8HT ...
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bioRxiv - Cell Biology 2020Quote: ... Fc receptors were blocked with human TruStain FcX (Biolegend, Table S6) for 30 minutes on ice ...
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bioRxiv - Immunology 2021Quote: ... APC-conjugated anti-human IgG Fc (clone HP6017) was from Biolegend. For analysis of CD47 expression on human PBMCs ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor® 647 anti-human CD326 (EpCAM) Antibody (Biolegend 324212), PE anti-human/mouse CD49f Antibody (Biolegend 313612) ...