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1601 - 1650 of 2921 citations for Recombinant Human FLT1 His tagged since 2019

• S100A8/A9 predicts triple-negative breast cancer response to PIM kinase and PD-1/PD-L1 inhibition

  Lauren R. Begg, et al., bioRxiv - Cancer Biology 2023

  Quote: ... mouse anti-human CD45 conjugated to FITC (clone HI30, Biolegend, 304005), mouse anti-human FOXP3 conjugated to BB700 (clone 236A/E7 ...
• Peculiar transcriptional reprogramming with functional impairment of dendritic cells upon exposure to transformed HTLV-1-infected cells

  Auriane Carcone, et al., bioRxiv - Microbiology 2024

  Quote: ... PE-Cy7-coupled mouse anti-Human ICOSL (BioLegend, 309410; 1/100), Mouse anti-Gagp19 clone TP7 (Zeptometrix ...
• GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior

  Aaqib M. Bhat, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and live cells stained with APC anti-human GD2 (#357306; Biolegend) (isotype control ...
• MAIT cells have a negative impact on GBM

  Seketoulie Keretsu, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were subsequently incubated with a cocktail containing Human TruStainFcX (Biolegend) and PBS-57/CD1d-tetramers (NIH Tetramer Facility ...
• Engineered 3D Hydrogel Matrices to Modulate Trophoblast Stem Cell-Derived Placental Organoid Phenotype

  Emily M. Slaby, et al., bioRxiv - Bioengineering 2024

  Quote: ... and APC anti-human EGFR antibody (BioLegend catalog #352906, clone AY13) at 1:100 dilutions in DPBS + 1% BSA for 15 minutes at room temperature in the dark ...
• Cell of origin alters myeloid-mediated immunosuppression in lung adenocarcinoma

  Minxiao Yang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... anti-mouse/human CD11b-PE/Dazzle 594 (1:120, 101256, Biolegend), and anti-mouse MHCII APC/Cyanine7 (1:120 ...
• Massively parallel interrogation of human functional variants modulating cancer immunosurveillance

  Ying Liu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... followed by staining with anti-human CD3 (UCHT1, BV650, BioLegend, #00467) and DAPI (BioLegend ...
• CD4 downregulation precedes Env expression and protects HIV-1-infected cells from ADCC mediated by non-neutralizing antibodies

  Jonathan Richard, et al., bioRxiv - Microbiology 2024

  Quote: ... FITC or PE-conjugated Mouse anti-human CD4 (clone OKT4; Biolegend) were used for cell-surface staining of HIV-1-infected primary CD4+ T cells ...
• Genome-wide screen of Mycobacterium tuberculosis-infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth

  Nelson V. Simwela, et al., bioRxiv - Immunology 2024

  Quote: ... and the PE anti-mouse/human CD11b antibody (Biolegend; 1:500) for 30 minutes at 40C ...
• Genome-wide screen of Mycobacterium tuberculosis-infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth

  Nelson V. Simwela, et al., bioRxiv - Immunology 2024

  Quote: ... and the PE anti-mouse/human CD11b antibody (Biolegend; 1:500) for 30 minutes at 40C ...
• Transcription of HIV-1 at sites of intact latent provirus integration

  Ana Rafaela Teixeira, et al., bioRxiv - Immunology 2024

  Quote: ... PerCP/Cyanine5.5 conjugated anti-human CD4 (clone OKT4, BioLegend, cat. 317428); BrillianViolet conjugated anti-human CD25 (clone BC56 ...
• Vimentin regulates mitochondrial ROS production and inflammatory responses of neutrophils

  Thao Ngoc Huynh, et al., bioRxiv - Immunology 2024

  Quote: ... and PE conjugated anti-mouse/human CD11b (1:200, Biolegend, 101207) for 30 minutes on ice ...
• Akt2 deficiency impairs Th17 differentiation, augments Th2 differentiation, and alters the peripheral response to immunization

  Lauren B. Banks, et al., bioRxiv - Immunology 2024

  Quote: ... AF700 rat anti-human/mouse CD44 (clone IM7, BioLegend, cat# 103026) (54) ...
• Synergically enhanced anti-tumor immunity of in vivo CAR by circRNA vaccine boosting

  Yanyan Wang, et al., bioRxiv - Immunology 2024

  Quote: ... or Alexa Fluor 647 anti-human CD206 (MMR) Antibody (BioLegend, #321116) in dark ...
• Neisseria gonorrhoeae induces local secretion of IL-10 at the human cervix to promote asymptomatic colonization

  Yiwei Dai, et al., bioRxiv - Cell Biology 2024

  Quote: ... or anti-human IL-10Rα antibody (5 μg/ml, 308802, BioLegend) for 24 h ...
• In-depth immunometabolic profiling by measuring cellular protein translation inhibition via bioorthogonal noncanonical amino acid tagging (CENCAT)

  Frank Vrieling, et al., bioRxiv - Immunology 2024

  Quote: ... CD14+ monocytes were magnetically labeled using MojoSort Human CD14 Nanobeads (Biolegend) and subsequently separated on LS columns using a QuadroMACS™ Separator (Miltenyi Biotec) ...
• Phenotype and function of IL-10 producing NK cells in individuals with malaria experience

  Sarah A. McNitt, et al., bioRxiv - Immunology 2024

  Quote: ... cells were analyzed using Biolegend’s LEGENDScreenTM Human PE Kit (Biolegend, 700007). Seven subjects were incubated with IL-15 and IL-21 (as above ...
• Toll-like receptor 2 orchestrates a potent anti-tumor response in non-small cell lung cancer

  Fraser R. Millar, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mice were anaesthetised as described above and intranasally inoculated with 100ng of carrier free recombinant protein (rIL-1α – Biolegend #575002, rIL-1β – Biolegend #575102) suspended in sterile phosphate buffered saline (PBS) ...
• Resources for genome editing in livestock: Cas9-expressing chickens and pigs

  Denise Bartsch, et al., bioRxiv - Molecular Biology 2020

  Quote: ... Organoids were cultivated in growth medium as previously described (61) with minor changes (50% mouse Wnt3a-conditioned medium, 50ng/mL mouse Wnt3a recombinant protein (Biolegend, USA), 15% mouse R-spondin1-conditioned medium ...
• Generation of dual specific bivalent BiTEs (dbBIspecific T-cell Engaging antibodies) for cellular immunotherapy

  Maciej Kujawski, et al., bioRxiv - Cancer Biology 2019

  Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
• Cancer cell CCR2 orchestrates suppression of the adaptive immune response

  Miriam R. Fein, et al., bioRxiv - Cancer Biology 2019

  Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
• Phagocytic “teeth” and myosin-II “jaw” power target constriction during phagocytosis

  Daan Vorselen, et al., bioRxiv - Cell Biology 2021

  Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
• A multi-enhancer hub at the Ets1 locus controls T cell differentiation and allergic inflammation through 3D genome topology

  Aditi Chandra, et al., bioRxiv - Genomics 2022

  Quote: ... 0.2×106 naïve CD4+ T cells were cultured in presence of 10 ng/mL of recombinant IL-12 (BioLegend, Cat#577002), 1 ng/mL of recombinant IL-2 (BioLegend ...
• Heparan sulfate promotes TRAIL-induced tumor cell apoptosis

  Yin Luo, et al., bioRxiv - Cell Biology 2023

  Quote: ... breast cancer cells and myeloma cells were treated with recombinant hTRAIL (B294007, Biolegend, 1 to 300 ng/ml for different cells) for 3 h in the presence or absence of either HL-III (5 milliunits/ml) ...
• ZEB1-dependent modulation of fibroblast polarization governs inflammation and immune checkpoint blockade sensitivity in colorectal cancer

  Harald Schuhwerk, et al., bioRxiv - Cancer Biology 2023

  Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
• An Alzheimer’s disease risk variant in TTC3 modifies the actin cytoskeleton organization and the PI3K-Akt signaling pathway in iPSC-derived forebrain neurons

  Holly N. Cukier, et al., bioRxiv - Genetics 2023

  Quote: ... Recombinant Anti-AKT1 + AKT2 + AKT3 (phospho S472 + S473 + S474, Purified anti-Tubulin β-3 (TUBB3) Antibody 1:1000 (Biolegend, # PRB-435P), anti-GAPDH antibody 1:1000 (Sigma Aldrich ...
• Intra- and extra-cellular environments contribute to the fate of HIV-1 infection

  Sneha Ratnapriya, et al., bioRxiv - Microbiology 2020

  Quote: ... Cells were stained using the anti-human monoclonal antibodies fluorophore-conjugated (Biolegend) Brilliant Violet 605™ anti-human CD45 (HI30) ...
• Human alveolar Type 2 epithelium transdifferentiates into metaplastic KRT5+ basal cells during alveolar repair

  Jaymin J. Kathiriya, et al., bioRxiv - Cell Biology 2020

  Quote: ... mouse anti-human CD326 PE (BioLegend; Cat#324206; used at 1:200), and mouse IgM anti-human HTII-280 (Terrace Biotech ...
• SARS-CoV-2 spike-specific memory B cells express markers of durable immunity after non-severe COVID-19 but not after severe disease

  Raphael A. Reyes, et al., bioRxiv - Immunology 2021

  Quote: ... 100 μl anti-human IgG or IgM - horseradish peroxidase (HRP) conjugates (BioLegend), diluted 1:2500 and 1:5000 in dilution buffer ...
• Broadly reactive human monoclonal antibodies targeting the pneumococcal histidine triad protein protect against fatal pneumococcal infection

  Jiachen Huang, et al., bioRxiv - Immunology 2021

  Quote: ... Antibody binding was detected using an APC Anti-Human IgG Fc (Biolegend) at a 1:100 dilution incubated for 1 hr with the bacteria ...
• Notch Signaling Commits Mesoderm to the Cardiac Lineage

  Evan S. Bardot, et al., bioRxiv - Developmental Biology 2020

  Quote: ... anti-human CD309 (KDR/VEG-FR2) (Biolegend, clone 7D4-6; 1:200); anti-human CD184 (CXCR4 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human FcER1 BV510 (1:100, clone AER-37, BioLegend, cat # 334626), Anti-human CD303 BV510 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD163 PE (1:50, clone GHI/61, BioLegend, cat # 333606), Anti-human CD49d PE Dazzle 594 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD56 APC Cy7 (1:50, clone HCD56, BioLegend, cat # 318332), Anti-human CD45 APC Cy7 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human PD-1 BV785 (1:50, clone EH12.2H7, BioLegend, cat # 329930), Anti-human CD45 BUV395 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD4 BV510 (1:100, clone RPA-T4, BioLegend, cat # 300546), Anti-human CD56 BV605 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human MerTK PE Cy7 (1:50, clone 590H11G1E3, BioLegend, cat # 367610), Anti-human TIGIT PE Cy 7 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD103 BV711 (1:100, clone Ber-ACT8, BioLegend, cat # 350222), Anti-human CD10 BV711 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD206 APC (1:50, clone 15-2, BioLegend, cat # 321110), Anti-human CD4 Alexa700 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human Granzyme K PE (1:25, clone GM26E7, BioLegend, cat # 370512), Anti-human FOXP3 PE CF594 (1:25 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CXCR6 PerCP Cy5.5 (1:50, clone K041E5, BioLegend, cat # 356010), Anti-human CCR8 PE (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CXCR4 APC Cy7 (1:50, clone 12G5, BioLegend, cat # 306528), Anti-human CTLA4 APC Cy7 (1:25 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD8a BV650 (1:50, clone RPA-T8, BioLegend, cat # 301042), Anti-human CD326 BV650 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CTLA4 APC Cy7 (1:25, clone BNI3, BioLegend, cat # 369634), Anti-human CD56 APC Cy7 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human HLA-DR BV785 (1:50, clone L243, BioLegend, cat # 307642), Anti-human PD-1 BV785 (1:50 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD45 APC Cy7 (1:100, clone HI30, BioLegend, cat # 304014), Anti-human CD14 APC eF780 (1:100 ...
• Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity

  Caitriona M. McEvoy, et al., bioRxiv - Immunology 2021

  Quote: ... Anti-human CD8a PerCP (1:50, clone RPA-T8, BioLegend, cat # 301030), Anti-human CXCR6 PerCP Cy5.5 (1:50 ...
• Human CD4+CD103+ cutaneous resident memory T cells are found in the circulation of healthy subjects

  Maria Magdalena Klicznik, et al., bioRxiv - Immunology 2019

  Quote: ... The cells were washed and resuspended with Human TruStain FcX (Biolegend; 422302) for five minutes before adding the primary surface staining cocktail for 20m ...
• CIS checkpoint deletion enhances the fitness of cord blood derived natural killer cells transduced with a chimeric antigen receptor

  May Daher, et al., bioRxiv - Immunology 2020

  Quote: ... Brilliant Violet 605™ anti-human CD56 (NCAM) Antibody (Clone HCD56, Biolegend), anti-CAR antibody Goat F(ab’)2 anti-Human IgG (H+L)-Alexa Fluor 647 (Jackson) ...
• PD-L1:CD80 Heterodimer Triggers CD28 While Repressing Both PD-1 and CTLA4 Pathways

  Yunlong Zhao, et al., bioRxiv - Immunology 2019

  Quote: ... cells were pre-incubated with Human TruStain FcX™ (BioLegend, catalog 422301) to block Fc receptors ...