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Citations for BioLegend :
1601 - 1650 of 3242 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-CD16/CD32 (clone 93, eBioscience, 14-0161-86) for Fc receptor blockade and stained with Zombie Yellow (Biolegend) for 10 minutes at room temperature in the dark to distinguish live and dead cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 400 g for 8 minutes and stained with lanthanide-labeled antibodies against immunophenotypic markers in CyTOF buffer containing Fc receptor blocker (BioLegend) for 30 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed three times in 1x PBS then stained for 15 minutes at 4°C in Fc block cocktail (1:20 Nova block, Phitonex, Mouse TruStain FcX, Biolegend #101320 ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2023Quote: All flow cytometry was performed at Fred Hutchison Cancer Center utilizing a 28-color panel focused on T cell phenotyping (Supplementary Table 1) [33] CVC and PBMC were incubated with Fc-blocking reagent (BioLegend) and fixable UV Blue Live/Dead reagent (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were resuspended in 200 uL PBS containing 2% FBS and Fc block and incubated with biotin anti-mouse F4/80 (Clone BM8, BioLegend), CD3 (Clone 500A2 ...
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bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
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bioRxiv - Immunology 2024Quote: ... cells were washed with FACS staining buffer (PBS containing 2% fetal bovine serum [FBS] and 1 mM EDTA) and incubated with an Fc receptor blocker (TrueStain fcX; BioLegend) for 5 minutes at 4℃ ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed and stained for 30min at 4°C in the dark with Fc block diluted 1:200 and subsets of the following antibodies: anti-CD103 (2E7, Biolegend), anti-CD11b (M1/70 ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Cancer Biology 2021Quote: ... The PE anti-human CD274/PD-L1 (Biolegend, catalog# 329706) antibody and the isotype control antibody (PE Mouse IgG2b ...
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bioRxiv - Cancer Biology 2021Quote: ... APC anti-human CD3 antibody (clone HIT3a, catalog #300319, BioLegend). The 10x library was sequenced on an Illumina HiSeq 2500 instrument.
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bioRxiv - Cell Biology 2020Quote: ... APC-Fire 750 anti-human CD20 (302357, clone 2H7, BioLegend), and APC-H7 anti-human CD3 (560275 ...
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bioRxiv - Immunology 2021Quote: ... and APC-conjugated mouse anti-human IgD (Biolegend, CA, USA). To determine the purity of CB-SC ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD309 (VEGFR2) (Biolegend, clone 7D4-6; 1:50); anti-human CD56 (NCAM ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-human CD117 (c-kit) (Biolegend, clone 104D2; 1:100); anti-human CD148 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... and 50 ng/ml recombinant human M-CSF (BioLegend #574806) for 6 days to obtain monocytes derived macrophages ...
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bioRxiv - Immunology 2021Quote: ... 5ng/ml or 50ng/ml recombinant human G-CSF (BioLegend) or mouse G-CSF (BioLegend) ...
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bioRxiv - Bioengineering 2019Quote: Devices stained with Alexa Fluor 647 anti-human CD31 (BioLegend) and Alexa Fluor 594 Phalloidin (ThermoFisher ...
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bioRxiv - Cancer Biology 2019Quote: ... or PerCP and human anti-CD19-PE were from BioLegend, BD Biosciences ...
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bioRxiv - Immunology 2019Quote: ... anti-mouse/human CD11b APC-Cy7 (clone M1/70, Biolegend), anti-mouse CD11c PE-eFluor 610 (clone N418 ...
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bioRxiv - Cancer Biology 2019Quote: ... Anti-human CD45 (clone: 2D1, # 368516) was obtained from BioLegend, San Diego ...
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bioRxiv - Cell Biology 2019Quote: ... PE/Cy7-conjugated anti-human CD24 (1:20; ML5; Biolegend), and APC-conjugated human anti-CD133 (1:11 ...
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bioRxiv - Immunology 2021Quote: ... or PE anti-human IgM (1:80 dilution; BioLegend #314507)) was added per well ...
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bioRxiv - Immunology 2021Quote: ... Then the samples were treated with Human TruStain FcX (Biolegend) to block Fc receptors ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse/human CD11b APC-Cy7 (clone M1/70, Biolegend), anti-mouse CD11c PE-eFluor 610 (clone N418 ...
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bioRxiv - Cancer Biology 2021Quote: human HLA-A,B,C-PE (W6/32, Biolegend 311406), FC ...
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bioRxiv - Bioengineering 2021Quote: ... and PE/Cyanine 7 anti-human CD4 antibody (#317414, Biolegend), APC/Cyanine 7 anti-human CD8 antibody (#344714 ...
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bioRxiv - Bioengineering 2020Quote: ... and LEGENDplex™ Human Inflammation Panel were acquired from BioLegend.
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bioRxiv - Bioengineering 2020Quote: Human IFNγ was quantified via sandwich ELISA assay (430107, Biolegend) and per the manufacturer’s recommended conditions.
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bioRxiv - Immunology 2020Quote: ... Cells were stained with custom TotalSeq-C human antibodies (BioLegend) before loading onto a Chromium Next GEM chip G ...
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bioRxiv - Immunology 2021Quote: ... PE anti-mouse/human GL7 antibody (BioLegend, catalog no. 144608), Brilliant Violet 605 anti-mouse CD95 (Fas ...
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bioRxiv - Microbiology 2021Quote: ... Cytokines were measured using human ELISA Max deluxe kits (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Cancer Biology 2021Quote: ... Brilliant Violet 785™ anti-human CD4 (BioLegend, cat. 317442) and Brilliant Violet 650™ anti-human CD69 (BioLegend ...